LOCUS       40924_39528        3629 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA441, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3629)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2014) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   4  (bases 1 to 3629)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3629)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2014) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            On Sep 30, 2014 this sequence version replaced JX560339.1.
FEATURES             Location/Qualifiers
     source          1..3629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             485..1273
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            1422..1530
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1624..2212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2269..3099)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3113..3464
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3537..3623)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttacgta tttaaatgaa ccttgaccga
      301 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
      361 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
      421 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
      481 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
      541 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
      601 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
      661 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
      721 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
      781 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
      841 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
      901 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
      961 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     1021 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     1081 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     1141 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     1201 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     1261 ggtagtcggc aaataagaca attgtccttt tccgctgcat aaccctgctt cggggtcatt
     1321 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     1381 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     1441 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     1501 aacgggaatc ctgctctgcg aggctggccg taggccggcc gataatctca tgaccaaaat
     1561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2041 agggagaaag gcggacaggc atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2221 caacgcggcc gtgaaaggca ggccggtccg tggtggccac ggcctctagg ccagatccag
     2281 cggcatctgg gttagtcgag cgcgggccgc ttcccatgtc tcaccagggc gagcctgttt
     2341 cgcgatctca gcatctgaaa tcttcccggc cttgcgcttc gctggggcct tacccaccgc
     2401 cttggcgggc ttcttcggtc caaaactgaa caacagatgt gtgaccttgc gcccggtctt
     2461 tcgctgcgcc cactccacct gtagcgggct gtgctcgttg atctgcgtca cggctggatc
     2521 aagcactcgc aacttgaagt ccttgatcga gggataccgg ccttccagtt gaaaccactt
     2581 tcgcagctgg tcaatttcta tttcgcgctg gccgatgctg tcccattgca tgagcagctc
     2641 gtaaagcctg atcgcgtggg tgctgtccat cttggccacg tcagccaagg cgtatttggt
     2701 gaactgtttg gtgagttccg tcaggtacgg cagcatgtct ttggtgaacc tgagttctac
     2761 acggccctca ccctcccggt agatgattgt ttgcacccag ccggtaatca tcacactcgg
     2821 tcttttcccc ttgccattgg gctcttgggt taaccggact tcccgccgtt tcaggcgcag
     2881 ggccgcttct ttgagctggt tgtaggaaga ttcgataggg acacccgcca tcgtcgctat
     2941 gtcctccgcc gtcactgaat acatcacttc atcggtgaca ggctcgctcc tcttcacctg
     3001 gctaatacag gccagaacga tccgctgttc ctgaacactg aggcgatacg cggcctcgac
     3061 cagggcattg cttttgtaaa ccattggggg tgaggccacg ttcgacattc cttgtgtata
     3121 aggggacact gtatctgcgt cccacaatac aacaaatccg tccctttaca acaacaaatc
     3181 cgtcccttct taacaacaaa tccgtccctt aatggcaaca aatccgtccc tttttaaact
     3241 ctacaggcca cggattacgt ggcctgtaga cgtcctaaaa ggtttaaaag ggaaaaggaa
     3301 gaaaagggtg gaaacgcaaa aaacgcacca ctacgtggcc ccgttggggc cgcatttgtg
     3361 cccctgaagg ggcgggggag gcgtctgggc aatccccgtt ttaccagtcc cctatcgccg
     3421 cctgagaggg cgcaggaagc gagtaatcag ggtatcgagg cggattcacc cttggcgtcc
     3481 aaccagcggc accagcggcg cctgagaggg gcgcgcccag ctgtctaggg cggcggattt
     3541 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3601 gactgagcct ttcgttttat ttgatgcct
//
