LOCUS       40924_39478        5120 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA351, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 5120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        297..399
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             400..1056
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1208..1316
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1330..2178)
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2168..3004)
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3518..4486)
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     promoter        complement(4510..4540)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     rep_origin      4596..4990
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5028..5114)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg agacgttgat
      301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      361 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      421 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      481 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      541 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      601 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      661 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      721 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      781 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      841 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      901 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      961 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1021 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg tccttttccg
     1081 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1141 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1201 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1261 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1321 ccggcctcag cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt
     1381 taccgtccag cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt
     1441 gcgcatggtc gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga
     1501 agccttgccg gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc
     1561 ggtttcgctg tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc
     1621 catgacggcc tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc
     1681 gctggcgtac tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat
     1741 ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc
     1801 gacctctgcc ccgatttcct ttgccagcgc ccgatagcta cctttgacca catggcattc
     1861 agcggtgacg gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt
     1921 cttgggttcc gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag
     1981 gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc
     2041 gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg
     2101 gaacaggccg ggggccagac agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt
     2161 gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg
     2221 gcttgagcac cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt
     2281 tggccttgct cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt
     2341 cctcggcctc ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca
     2401 gtaccgagct gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct
     2461 tgctggcatg gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc
     2521 gaccgatgac ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca
     2581 gcgtgtccag caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact
     2641 ccggggccat gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca
     2701 cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg
     2761 cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct
     2821 ccagcagatc cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac
     2881 cggcaccacc gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt
     2941 agtccagcgg tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag
     3001 ccattgattg cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc
     3061 gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt
     3121 cagccagaac ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg
     3181 gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat
     3241 atcagtcacc gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg
     3301 tgcagccgtc aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct
     3361 tgtttgacgt ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt
     3421 agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact
     3481 catagtataa caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc
     3541 tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga
     3601 cgctgggcag acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg
     3661 gcttgcgctc tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac
     3721 tgcggccccg gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac
     3781 cgaagcgctt gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt
     3841 ggcggctaag ctgccgctcg ggcagttcga ggctggccag cctgcgggcc ttctcctgct
     3901 gccgctgggc ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg
     3961 cggtcttgcc cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt
     4021 gcttgtcctt gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg
     4081 ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc
     4141 ctgcggcgtc ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca
     4201 gggcaggctc ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca
     4261 ccagcaccag accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg
     4321 cattcatccg cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt
     4381 tcatcatctg gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg
     4441 ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg
     4501 attcccacac attatacgag ccggaagcat aaagtgtaaa gcctagatcc gaaggatgag
     4561 ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc
     4621 gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt
     4681 ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg
     4741 tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag
     4801 ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc
     4861 atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg
     4921 ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg
     4981 caggggttcg tgacagttag ggcgcgccca gctgtctagg gcggcggatt tgtcctactc
     5041 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     5101 tttcgtttta tttgatgcct
//
