LOCUS       40924_39473        3864 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA343, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3864)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3864)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        746..848
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             849..1505
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1657..1765
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1859..2447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2504..3334)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3348..3699
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3772..3858)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttaaattg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata
      781 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      841 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      901 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      961 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1021 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
     1081 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1141 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1201 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1261 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     1321 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1381 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1441 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1501 gggcgtaatt tgacttttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc
     1561 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     1621 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
     1681 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
     1741 gaatcctgct ctgcgaggct ggccgtaggc cggccgataa tctcatgacc aaaatccctt
     1801 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1861 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1921 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1981 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2041 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2101 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2161 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2221 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2281 gaaaggcgga caggcatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2341 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2401 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2461 cggccgtgaa aggcaggccg gtccgtggtg gccacggcct ctaggccaga tccagcggca
     2521 tctgggttag tcgagcgcgg gccgcttccc atgtctcacc agggcgagcc tgtttcgcga
     2581 tctcagcatc tgaaatcttc ccggccttgc gcttcgctgg ggccttaccc accgccttgg
     2641 cgggcttctt cggtccaaaa ctgaacaaca gatgtgtgac cttgcgcccg gtctttcgct
     2701 gcgcccactc cacctgtagc gggctgtgct cgttgatctg cgtcacggct ggatcaagca
     2761 ctcgcaactt gaagtccttg atcgagggat accggccttc cagttgaaac cactttcgca
     2821 gctggtcaat ttctatttcg cgctggccga tgctgtccca ttgcatgagc agctcgtaaa
     2881 gcctgatcgc gtgggtgctg tccatcttgg ccacgtcagc caaggcgtat ttggtgaact
     2941 gtttggtgag ttccgtcagg tacggcagca tgtctttggt gaacctgagt tctacacggc
     3001 cctcaccctc ccggtagatg attgtttgca cccagccggt aatcatcaca ctcggtcttt
     3061 tccccttgcc attgggctct tgggttaacc ggacttcccg ccgtttcagg cgcagggccg
     3121 cttctttgag ctggttgtag gaagattcga tagggacacc cgccatcgtc gctatgtcct
     3181 ccgccgtcac tgaatacatc acttcatcgg tgacaggctc gctcctcttc acctggctaa
     3241 tacaggccag aacgatccgc tgttcctgaa cactgaggcg atacgcggcc tcgaccaggg
     3301 cattgctttt gtaaaccatt gggggtgagg ccacgttcga cattccttgt gtataagggg
     3361 acactgtatc tgcgtcccac aatacaacaa atccgtccct ttacaacaac aaatccgtcc
     3421 cttcttaaca acaaatccgt cccttaatgg caacaaatcc gtcccttttt aaactctaca
     3481 ggccacggat tacgtggcct gtagacgtcc taaaaggttt aaaagggaaa aggaagaaaa
     3541 gggtggaaac gcaaaaaacg caccactacg tggccccgtt ggggccgcat ttgtgcccct
     3601 gaaggggcgg gggaggcgtc tgggcaatcc ccgttttacc agtcccctat cgccgcctga
     3661 gagggcgcag gaagcgagta atcagggtat cgaggcggat tcacccttgg cgtccaacca
     3721 gcggcaccag cggcgcctga gaggggcgcg cccagctgtc tagggcggcg gatttgtcct
     3781 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     3841 agcctttcgt tttatttgat gcct
//
