LOCUS       40924_39463        3415 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA341, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3415)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3415)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        297..399
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             400..1056
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1208..1316
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1410..1998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2055..2885)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      2899..3250
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3323..3409)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg agacgttgat
      301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      361 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      421 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      481 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      541 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      601 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      661 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      721 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      781 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      841 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      901 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      961 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1021 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg tccttttccg
     1081 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1141 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1201 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1261 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1321 ccggccgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggcatcc ggtaagcggc
     1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1981 gggcggagcc tatggaaaaa cgccagcaac gcggccgtga aaggcaggcc ggtccgtggt
     2041 ggccacggcc tctaggccag atccagcggc atctgggtta gtcgagcgcg ggccgcttcc
     2101 catgtctcac cagggcgagc ctgtttcgcg atctcagcat ctgaaatctt cccggccttg
     2161 cgcttcgctg gggccttacc caccgccttg gcgggcttct tcggtccaaa actgaacaac
     2221 agatgtgtga ccttgcgccc ggtctttcgc tgcgcccact ccacctgtag cgggctgtgc
     2281 tcgttgatct gcgtcacggc tggatcaagc actcgcaact tgaagtcctt gatcgaggga
     2341 taccggcctt ccagttgaaa ccactttcgc agctggtcaa tttctatttc gcgctggccg
     2401 atgctgtccc attgcatgag cagctcgtaa agcctgatcg cgtgggtgct gtccatcttg
     2461 gccacgtcag ccaaggcgta tttggtgaac tgtttggtga gttccgtcag gtacggcagc
     2521 atgtctttgg tgaacctgag ttctacacgg ccctcaccct cccggtagat gattgtttgc
     2581 acccagccgg taatcatcac actcggtctt ttccccttgc cattgggctc ttgggttaac
     2641 cggacttccc gccgtttcag gcgcagggcc gcttctttga gctggttgta ggaagattcg
     2701 atagggacac ccgccatcgt cgctatgtcc tccgccgtca ctgaatacat cacttcatcg
     2761 gtgacaggct cgctcctctt cacctggcta atacaggcca gaacgatccg ctgttcctga
     2821 acactgaggc gatacgcggc ctcgaccagg gcattgcttt tgtaaaccat tgggggtgag
     2881 gccacgttcg acattccttg tgtataaggg gacactgtat ctgcgtccca caatacaaca
     2941 aatccgtccc tttacaacaa caaatccgtc ccttcttaac aacaaatccg tcccttaatg
     3001 gcaacaaatc cgtccctttt taaactctac aggccacgga ttacgtggcc tgtagacgtc
     3061 ctaaaaggtt taaaagggaa aaggaagaaa agggtggaaa cgcaaaaaac gcaccactac
     3121 gtggccccgt tggggccgca tttgtgcccc tgaaggggcg ggggaggcgt ctgggcaatc
     3181 cccgttttac cagtccccta tcgccgcctg agagggcgca ggaagcgagt aatcagggta
     3241 tcgaggcgga ttcacccttg gcgtccaacc agcggcacca gcggcgcctg agaggggcgc
     3301 gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3361 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcct
//
