LOCUS       40924_39458        4926 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA338, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Calles B, Chavarria M, 
            Arce-Rodriguez A, de Las Heras A, Paez-Espino AD, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for the analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Nucleic Acids Res. 41 (DATABASE ISSUE), D666-D675 (2013)
  PUBMED    23180763
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2013) Systems Biology Program, CNB-CSIC, Darwin 3,
            Madrid, Madrid 28029, Spain
REFERENCE   3  (bases 1 to 4926)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4926)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 41 (DATABASE ISSUE), D666-D675 (2013)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-APR-2013) Systems Biology Program, CNB-CSIC, Darwin 3, Madrid, 
            Madrid 28029, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(22..984)
                     /codon_start=1
                     /label=XylS
                     /note="XylS regulator encoded by the Pseudomonas putida TOL
                     plasmid pWWO"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        1884..1964
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     misc_feature    2014..2070
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2079..2102)
                     /label=F24
                     /note="F24"
     terminator      2113..2207
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        2255..2357
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2358..3014
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            3166..3274
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3288..3947)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(3948..4719)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(4834..4920)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaac gttcgtaatc aagccacttc ctttttgcat tgacgcaggg tgtcggaagg
       61 caactcgccg aacgcgctcc tatagttttc agcgaagcgt cccaaatgta agaagccgta
      121 gtctagggct atctcagtta tactacgcac attggcactg ggatcgttca agcaggcgcg
      181 gatgctttcg agcttgcggt tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc
      241 gaacaaattg tagagcgagc gtggactcat catcgccagc tccgctaacc gctcaaggct
      301 gatattccgt ttgagattct cctcaatgaa ttgaacgact cgctcgaaag acgggttacc
      361 tttgctgaaa atttcacggc tgacattgct gcccagcatt tcgagcagct tggaagcgat
      421 gatccccgca tagtgctctt ggacccgagg catcgacttt gtatgttccg cttcgtcaca
      481 aactaacccg agtagattga taaagccatc gagttgctgg agattgtgtc gcgcggcgaa
      541 acggataccc tccctcggct tgtgccaatt gttgtcactg cacgcccgat caaggaccac
      601 tgagggcaat ttaacgataa atttctcgca atcttctgaa taggtcaggt cggcttggtc
      661 atccggattg agcagcaata gttcgcccgg cgcaaaatag tgctcctggc catggccacg
      721 ccacaggcaa tggcctttga gtattatttg cagatgataa caggtctcta atccaggcga
      781 gattaccctc acgctaccgc cgtagctgat tcgacacaga tcgaggcatc cgaagattct
      841 gtggtgcagc ctgcctgccg ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac
      901 atactggtta acataatcgg agactgcata gggctcggcg tggacgaaga tctgactttt
      961 ctcgttcaat aagcaaaaat ccatagttca cggttctctt attttaatgt gggctgcttg
     1021 gtgtgatgta gaaaggcgcc aagtcgatga aaatgcatct cgacgtgatg cgtatacggg
     1081 ttacccccat tgccacgttg cgccatcctt tttgcaatca gtgaccactt ttccaagcaa
     1141 aaataacgcc aagcagaacg aagacgttct ttttaagaag cgagaacacc agaagttcgt
     1201 gctgtcgggg catggggcga cgaattggcg gataaagggg atctgctgga tattacggcc
     1261 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1321 gcccgcctga tgaatgctca tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg
     1381 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1441 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1501 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1561 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     1621 gacaacttct tcgcccccgt tttcaccatg catgggaatt agcttgatct gaccaacgac
     1681 cggtagcgga gctatccaac ggcggtatac caggaaaaca cacagcaggt acatcagaac
     1741 agtaccatga ctgaagaaca aatagttttt tcctgatcca taaagcagaa cggcctgctc
     1801 catgacaaat ctggctcccc aactaatgcc ccatgcagcc agcataacca gcataaagtg
     1861 cagtgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca ggagtgcaaa
     1921 aaatggctat ctctagtaag gcctacccct taggctttat gcaacagaaa caataataat
     1981 ggagtcatga ccatgcctag gccgcggccg cgcgaattcg agctcggtac ccggggatcc
     2041 tctagagtcg acctgcaggc atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg
     2101 cgactagtct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     2161 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca ggggtcccca
     2221 ataattacga tttaaattgg cgaaaatgag acgttgatcg gcacgtaaga ggttccaact
     2281 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     2341 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     2401 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     2461 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2521 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     2581 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     2641 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     2701 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     2761 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     2821 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     2881 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2941 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3001 ggcagggcgg ggcgtaattt gacttttgtc cttttccgct gcataaccct gcttcggggt
     3061 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
     3121 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
     3181 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
     3241 gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccctaccg gcgcggcagc
     3301 gttacccgtg tcggcggctc caacggctcg ccatcgtcca gaaaacacgg ctcatcgggc
     3361 atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa ggctggcagg
     3421 tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg gccggtcggt
     3481 agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg ccccaacagc
     3541 gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga caggcgcaac
     3601 tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga cacggtgccg
     3661 gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt gactgcgtat
     3721 tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct gaccaccacg
     3781 gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc cgtgggtttc
     3841 ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac ccagtgaccg
     3901 ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct tatctccatg
     3961 cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg
     4021 cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg
     4081 caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg
     4141 ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg
     4201 cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc
     4261 ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc
     4321 ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccctt
     4381 cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc
     4441 gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg
     4501 cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg
     4561 ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc
     4621 aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt
     4681 tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat
     4741 ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt
     4801 agggggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac
     4861 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
     4921 atgcct
//
