LOCUS       40924_39438        3668 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA321, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3668)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        297..399
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             400..1056
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     oriT            1208..1316
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1380..2525)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(2918..3449)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(3576..3662)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg agacgttgat
      301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      361 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      421 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      481 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      541 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      601 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      661 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      721 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      781 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      841 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
      901 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
      961 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     1021 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg tccttttccg
     1081 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1141 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1201 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1261 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1321 ccggccgcga tgcaggtggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
     1381 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
     1441 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
     1501 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
     1561 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
     1621 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
     1681 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
     1741 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
     1801 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
     1861 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
     1921 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
     1981 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
     2041 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
     2101 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
     2161 aacgcggcct gcttggcttc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
     2221 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
     2281 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
     2341 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggactat cgagccgacg
     2401 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
     2461 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
     2521 ttcattcacc ctccttgcgg gattgccccg gaattaattc cccggatcga tccgtcgatc
     2581 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg
     2641 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata
     2701 aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg
     2761 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc
     2821 gtttctgcgg actggctttc tacgtggctg ccatttttgg ggtgaggccg ttcgcggccg
     2881 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
     2941 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
     3001 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
     3061 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
     3121 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
     3181 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
     3241 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
     3301 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
     3361 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
     3421 tccgcgaggt atccacaacg ccggcggccc tacatggctc tgctgtagtg agtgggttgc
     3481 gctccggcag cggtcctgat cccccgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3541 ttttctacgg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc
     3601 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     3661 tgatgcct
//
