LOCUS       40924_39413        4263 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA247R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4263)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..834
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      848..942
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1074..1886
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2056..2164
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2258..2846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2903..3733)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3747..4098
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4171..4257)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      181 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      301 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      361 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      421 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      481 caagacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      541 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      601 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      661 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      721 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      781 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaact
      841 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      901 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      961 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     1021 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     1081 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
     1141 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
     1201 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
     1261 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
     1321 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
     1381 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
     1441 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
     1501 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
     1561 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
     1621 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
     1681 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
     1741 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1801 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1861 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc gcgcgttgtc
     1921 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     1981 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     2041 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     2101 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     2161 gccgtaggcc ggccgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2401 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2641 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggcatccgg
     2701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccgtgaaa ggcaggccgg
     2881 tccgtggtgg ccacggcctc taggccagat ccagcggcat ctgggttagt cgagcgcggg
     2941 ccgcttccca tgtctcacca gggcgagcct gtttcgcgat ctcagcatct gaaatcttcc
     3001 cggccttgcg cttcgctggg gccttaccca ccgccttggc gggcttcttc ggtccaaaac
     3061 tgaacaacag atgtgtgacc ttgcgcccgg tctttcgctg cgcccactcc acctgtagcg
     3121 ggctgtgctc gttgatctgc gtcacggctg gatcaagcac tcgcaacttg aagtccttga
     3181 tcgagggata ccggccttcc agttgaaacc actttcgcag ctggtcaatt tctatttcgc
     3241 gctggccgat gctgtcccat tgcatgagca gctcgtaaag cctgatcgcg tgggtgctgt
     3301 ccatcttggc cacgtcagcc aaggcgtatt tggtgaactg tttggtgagt tccgtcaggt
     3361 acggcagcat gtctttggtg aacctgagtt ctacacggcc ctcaccctcc cggtagatga
     3421 ttgtttgcac ccagccggta atcatcacac tcggtctttt ccccttgcca ttgggctctt
     3481 gggttaaccg gacttcccgc cgtttcaggc gcagggccgc ttctttgagc tggttgtagg
     3541 aagattcgat agggacaccc gccatcgtcg ctatgtcctc cgccgtcact gaatacatca
     3601 cttcatcggt gacaggctcg ctcctcttca cctggctaat acaggccaga acgatccgct
     3661 gttcctgaac actgaggcga tacgcggcct cgaccagggc attgcttttg taaaccattg
     3721 ggggtgaggc cacgttcgac attccttgtg tataagggga cactgtatct gcgtcccaca
     3781 atacaacaaa tccgtccctt tacaacaaca aatccgtccc ttcttaacaa caaatccgtc
     3841 ccttaatggc aacaaatccg tcccttttta aactctacag gccacggatt acgtggcctg
     3901 tagacgtcct aaaaggttta aaagggaaaa ggaagaaaag ggtggaaacg caaaaaacgc
     3961 accactacgt ggccccgttg gggccgcatt tgtgcccctg aaggggcggg ggaggcgtct
     4021 gggcaatccc cgttttacca gtcccctatc gccgcctgag agggcgcagg aagcgagtaa
     4081 tcagggtatc gaggcggatt cacccttggc gtccaaccag cggcaccagc ggcgcctgag
     4141 aggggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     4201 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     4261 cct
//
