LOCUS       40924_39408        4230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA247D, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..801
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     terminator      815..909
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1041..1853
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2023..2131
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2225..2813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2870..3700)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3714..4065
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4138..4224)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatgg cctcctccga gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg
      181 gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      241 gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      301 gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac
      361 atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      421 ttcgaggacg gcggcgtggc gaccgtgacc caggactcct ccctgcaaga cggctgcttc
      481 atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt gatgcagaag
      541 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
      601 ggcgagaccc acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
      661 tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag
      721 ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag
      781 ggccgccacc acctgttcct gtagactagt cttggactcc tgttgataga tccagtaatg
      841 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      901 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
      961 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1021 acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc
     1081 gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg
     1141 gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc
     1201 tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact
     1261 ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg
     1321 cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc
     1381 cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca
     1441 ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga
     1501 gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc
     1561 cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg
     1621 tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct
     1681 gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga
     1741 actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg
     1801 ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat
     1861 taattggacc gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat
     1921 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
     1981 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
     2041 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
     2101 caacgggaat cctgctctgc gaggctggcc gtaggccggc cgataatctc atgaccaaaa
     2161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2641 aagggagaaa ggcggacagg catccggtaa gcggcagggt cggaacagga gagcgcacga
     2701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2821 gcaacgcggc cgtgaaaggc aggccggtcc gtggtggcca cggcctctag gccagatcca
     2881 gcggcatctg ggttagtcga gcgcgggccg cttcccatgt ctcaccaggg cgagcctgtt
     2941 tcgcgatctc agcatctgaa atcttcccgg ccttgcgctt cgctggggcc ttacccaccg
     3001 ccttggcggg cttcttcggt ccaaaactga acaacagatg tgtgaccttg cgcccggtct
     3061 ttcgctgcgc ccactccacc tgtagcgggc tgtgctcgtt gatctgcgtc acggctggat
     3121 caagcactcg caacttgaag tccttgatcg agggataccg gccttccagt tgaaaccact
     3181 ttcgcagctg gtcaatttct atttcgcgct ggccgatgct gtcccattgc atgagcagct
     3241 cgtaaagcct gatcgcgtgg gtgctgtcca tcttggccac gtcagccaag gcgtatttgg
     3301 tgaactgttt ggtgagttcc gtcaggtacg gcagcatgtc tttggtgaac ctgagttcta
     3361 cacggccctc accctcccgg tagatgattg tttgcaccca gccggtaatc atcacactcg
     3421 gtcttttccc cttgccattg ggctcttggg ttaaccggac ttcccgccgt ttcaggcgca
     3481 gggccgcttc tttgagctgg ttgtaggaag attcgatagg gacacccgcc atcgtcgcta
     3541 tgtcctccgc cgtcactgaa tacatcactt catcggtgac aggctcgctc ctcttcacct
     3601 ggctaataca ggccagaacg atccgctgtt cctgaacact gaggcgatac gcggcctcga
     3661 ccagggcatt gcttttgtaa accattgggg gtgaggccac gttcgacatt ccttgtgtat
     3721 aaggggacac tgtatctgcg tcccacaata caacaaatcc gtccctttac aacaacaaat
     3781 ccgtcccttc ttaacaacaa atccgtccct taatggcaac aaatccgtcc ctttttaaac
     3841 tctacaggcc acggattacg tggcctgtag acgtcctaaa aggtttaaaa gggaaaagga
     3901 agaaaagggt ggaaacgcaa aaaacgcacc actacgtggc cccgttgggg ccgcatttgt
     3961 gcccctgaag gggcggggga ggcgtctggg caatccccgt tttaccagtc ccctatcgcc
     4021 gcctgagagg gcgcaggaag cgagtaatca gggtatcgag gcggattcac ccttggcgtc
     4081 caaccagcgg caccagcggc gcctgagagg ggcgcgccca gctgtctagg gcggcggatt
     4141 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     4201 cgactgagcc tttcgtttta tttgatgcct
//
