LOCUS       40924_39403        4272 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA247C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4272)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..843
                     /codon_start=1
                     /label=ECFP
                     /note="enhanced CFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      857..951
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1083..1895
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2065..2173
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2912..3742)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3756..4107
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4180..4266)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      181 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
      361 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
      601 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      661 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      721 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      781 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      841 aagtaaacta gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      901 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc
      961 cccaataatt acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata
     1021 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1081 ttatgagcca tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg
     1141 atgcggatct gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca
     1201 tttatcgtct gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca
     1261 gcgtggcgaa cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc
     1321 cgctgccgac cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg
     1381 cgattccggg caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca
     1441 ttgtggatgc gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc
     1501 cgtttaacag cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc
     1561 tggtggatgc gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga
     1621 aagaaatgca taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta
     1681 gcctggataa cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg
     1741 tgggcattgc ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta
     1801 gcccgagcct gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca
     1861 aactgcaatt tcatctgatg ctggatgaat ttttctaata attaattgga ccgcggtccg
     1921 cgcgttgtcc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt
     1981 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc
     2041 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
     2101 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
     2161 gcgaggctgg ccgtaggccg gccgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggcatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gccgtgaaag
     2881 gcaggccggt ccgtggtggc cacggcctct aggccagatc cagcggcatc tgggttagtc
     2941 gagcgcgggc cgcttcccat gtctcaccag ggcgagcctg tttcgcgatc tcagcatctg
     3001 aaatcttccc ggccttgcgc ttcgctgggg ccttacccac cgccttggcg ggcttcttcg
     3061 gtccaaaact gaacaacaga tgtgtgacct tgcgcccggt ctttcgctgc gcccactcca
     3121 cctgtagcgg gctgtgctcg ttgatctgcg tcacggctgg atcaagcact cgcaacttga
     3181 agtccttgat cgagggatac cggccttcca gttgaaacca ctttcgcagc tggtcaattt
     3241 ctatttcgcg ctggccgatg ctgtcccatt gcatgagcag ctcgtaaagc ctgatcgcgt
     3301 gggtgctgtc catcttggcc acgtcagcca aggcgtattt ggtgaactgt ttggtgagtt
     3361 ccgtcaggta cggcagcatg tctttggtga acctgagttc tacacggccc tcaccctccc
     3421 ggtagatgat tgtttgcacc cagccggtaa tcatcacact cggtcttttc cccttgccat
     3481 tgggctcttg ggttaaccgg acttcccgcc gtttcaggcg cagggccgct tctttgagct
     3541 ggttgtagga agattcgata gggacacccg ccatcgtcgc tatgtcctcc gccgtcactg
     3601 aatacatcac ttcatcggtg acaggctcgc tcctcttcac ctggctaata caggccagaa
     3661 cgatccgctg ttcctgaaca ctgaggcgat acgcggcctc gaccagggca ttgcttttgt
     3721 aaaccattgg gggtgaggcc acgttcgaca ttccttgtgt ataaggggac actgtatctg
     3781 cgtcccacaa tacaacaaat ccgtcccttt acaacaacaa atccgtccct tcttaacaac
     3841 aaatccgtcc cttaatggca acaaatccgt ccctttttaa actctacagg ccacggatta
     3901 cgtggcctgt agacgtccta aaaggtttaa aagggaaaag gaagaaaagg gtggaaacgc
     3961 aaaaaacgca ccactacgtg gccccgttgg ggccgcattt gtgcccctga aggggcgggg
     4021 gaggcgtctg ggcaatcccc gttttaccag tcccctatcg ccgcctgaga gggcgcagga
     4081 agcgagtaat cagggtatcg aggcggattc acccttggcg tccaaccagc ggcaccagcg
     4141 gcgcctgaga ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     4201 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     4261 tatttgatgc ct
//
