LOCUS       40924_39393        6711 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA245, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 6711)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..3198
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     terminator      3296..3390
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3522..4334
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            4504..4612
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4706..5294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5351..6181)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      6195..6546
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(6619..6705)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa
      181 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      241 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      301 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
      361 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
      421 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
      481 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
      541 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
      601 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
      661 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg
      721 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtgtcgtt gctgcataaa
      781 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
      841 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
      901 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
      961 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     1021 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     1081 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     1141 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     1201 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     1261 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     1321 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     1381 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     1441 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     1501 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     1561 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     1621 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     1681 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     1741 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     1801 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     1861 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     1921 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     1981 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     2041 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tgctgcactg gatggtggcg
     2101 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     2161 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     2221 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     2281 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     2341 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     2401 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     2461 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     2521 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     2581 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     2641 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     2701 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     2761 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     2821 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     2881 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     2941 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     3001 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     3061 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     3121 tcctggagcc cgtcagtatc ggcggagttc cagctgagcg ccggtcgcta ccattaccag
     3181 ttggtctggt gtcaaaaata ataataaccg ggcaggccat gtctgcccgt atttcgcgta
     3241 aggaaatcca ttatgtacta tttaaaatca agctccttgc aggccactag tcttggactc
     3301 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     3361 tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat
     3421 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     3481 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcagcgtg
     3541 aaacgagctg tagccgtccg cgtctgaaca gcaacatgga tgcggatctg tatggctata
     3601 aatgggcgcg tgataacgtg ggtcagagcg gcgcgaccat ttatcgtctg tatggcaaac
     3661 cggatgcgcc ggaactgttt ctgaaacatg gcaaaggcag cgtggcgaac gatgtgaccg
     3721 atgaaatggt gcgtctgaac tggctgaccg aatttatgcc gctgccgacc attaaacatt
     3781 ttattcgcac cccggatgat gcgtggctgc tgaccaccgc gattccgggc aaaaccgcgt
     3841 ttcaggtgct ggaagaatat ccggatagcg gcgaaaacat tgtggatgcg ctggccgtgt
     3901 ttctgcgtcg tctgcatagc attccggtgt gcaactgccc gtttaacagc gatcgtgtgt
     3961 ttcgtctggc ccaggcgcag agccgtatga acaacggcct ggtggatgcg agcgattttg
     4021 atgatgaacg taacggctgg ccggtggaac aggtgtggaa agaaatgcat aaactgctgc
     4081 cgtttagccc ggatagcgtg gtgacccacg gcgattttag cctggataac ctgattttcg
     4141 atgaaggcaa actgattggc tgcattgatg tgggccgtgt gggcattgcg gatcgttatc
     4201 aggatctggc cattctgtgg aactgcctgg gcgaatttag cccgagcctg caaaaacgtc
     4261 tgtttcagaa atatggcatt gataatccgg atatgaacaa actgcaattt catctgatgc
     4321 tggatgaatt tttctaataa ttaattggac cgcggtccgc gcgttgtcct tttccgctgc
     4381 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     4441 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     4501 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     4561 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     4621 ccgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     4861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gcatccggta agcggcaggg
     5161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5281 ggagcctatg gaaaaacgcc agcaacgcgg ccgtgaaagg caggccggtc cgtggtggcc
     5341 acggcctcta ggccagatcc agcggcatct gggttagtcg agcgcgggcc gcttcccatg
     5401 tctcaccagg gcgagcctgt ttcgcgatct cagcatctga aatcttcccg gccttgcgct
     5461 tcgctggggc cttacccacc gccttggcgg gcttcttcgg tccaaaactg aacaacagat
     5521 gtgtgacctt gcgcccggtc tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt
     5581 tgatctgcgt cacggctgga tcaagcactc gcaacttgaa gtccttgatc gagggatacc
     5641 ggccttccag ttgaaaccac tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc
     5701 tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca
     5761 cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt
     5821 ctttggtgaa cctgagttct acacggccct caccctcccg gtagatgatt gtttgcaccc
     5881 agccggtaat catcacactc ggtcttttcc ccttgccatt gggctcttgg gttaaccgga
     5941 cttcccgccg tttcaggcgc agggccgctt ctttgagctg gttgtaggaa gattcgatag
     6001 ggacacccgc catcgtcgct atgtcctccg ccgtcactga atacatcact tcatcggtga
     6061 caggctcgct cctcttcacc tggctaatac aggccagaac gatccgctgt tcctgaacac
     6121 tgaggcgata cgcggcctcg accagggcat tgcttttgta aaccattggg ggtgaggcca
     6181 cgttcgacat tccttgtgta taaggggaca ctgtatctgc gtcccacaat acaacaaatc
     6241 cgtcccttta caacaacaaa tccgtccctt cttaacaaca aatccgtccc ttaatggcaa
     6301 caaatccgtc cctttttaaa ctctacaggc cacggattac gtggcctgta gacgtcctaa
     6361 aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca aaaaacgcac cactacgtgg
     6421 ccccgttggg gccgcatttg tgcccctgaa ggggcggggg aggcgtctgg gcaatccccg
     6481 ttttaccagt cccctatcgc cgcctgagag ggcgcaggaa gcgagtaatc agggtatcga
     6541 ggcggattca cccttggcgt ccaaccagcg gcaccagcgg cgcctgagag gggcgcgccc
     6601 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     6661 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
