LOCUS       40924_39388        4130 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA243Y, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4130)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      715..809
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             941..1753
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1923..2031
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2125..2713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2770..3600)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3614..3965
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4038..4124)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccaat cgtctcagag acacctgcga tggagaagac
      301 ttgagaggtc tcagagaatt ggatatctca gactcattgt cttctcatga gacctcacat
      361 cgcaggtgct caggagacgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
      421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      541 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      601 acgcatctgt gcggtatttc acaccgcata tgatgcactc tcagtacaat ctgctctgat
      661 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgaactagt cttggactcc
      721 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      781 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
      841 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      901 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcagcgtga
      961 aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt atggctataa
     1021 atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc
     1081 ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga
     1141 tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca ttaaacattt
     1201 tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca aaaccgcgtt
     1261 tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt
     1321 tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt
     1381 tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga gcgattttga
     1441 tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc
     1501 gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc tgattttcga
     1561 tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg atcgttatca
     1621 ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc aaaaacgtct
     1681 gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc atctgatgct
     1741 ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt ttccgctgca
     1801 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     1861 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     1921 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     1981 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc
     2041 cgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2101 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2161 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2281 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2341 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2401 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2461 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2521 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg catccggtaa gcggcagggt
     2581 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2641 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2701 gagcctatgg aaaaacgcca gcaacgcggc cgtgaaaggc aggccggtcc gtggtggcca
     2761 cggcctctag gccagatcca gcggcatctg ggttagtcga gcgcgggccg cttcccatgt
     2821 ctcaccaggg cgagcctgtt tcgcgatctc agcatctgaa atcttcccgg ccttgcgctt
     2881 cgctggggcc ttacccaccg ccttggcggg cttcttcggt ccaaaactga acaacagatg
     2941 tgtgaccttg cgcccggtct ttcgctgcgc ccactccacc tgtagcgggc tgtgctcgtt
     3001 gatctgcgtc acggctggat caagcactcg caacttgaag tccttgatcg agggataccg
     3061 gccttccagt tgaaaccact ttcgcagctg gtcaatttct atttcgcgct ggccgatgct
     3121 gtcccattgc atgagcagct cgtaaagcct gatcgcgtgg gtgctgtcca tcttggccac
     3181 gtcagccaag gcgtatttgg tgaactgttt ggtgagttcc gtcaggtacg gcagcatgtc
     3241 tttggtgaac ctgagttcta cacggccctc accctcccgg tagatgattg tttgcaccca
     3301 gccggtaatc atcacactcg gtcttttccc cttgccattg ggctcttggg ttaaccggac
     3361 ttcccgccgt ttcaggcgca gggccgcttc tttgagctgg ttgtaggaag attcgatagg
     3421 gacacccgcc atcgtcgcta tgtcctccgc cgtcactgaa tacatcactt catcggtgac
     3481 aggctcgctc ctcttcacct ggctaataca ggccagaacg atccgctgtt cctgaacact
     3541 gaggcgatac gcggcctcga ccagggcatt gcttttgtaa accattgggg gtgaggccac
     3601 gttcgacatt ccttgtgtat aaggggacac tgtatctgcg tcccacaata caacaaatcc
     3661 gtccctttac aacaacaaat ccgtcccttc ttaacaacaa atccgtccct taatggcaac
     3721 aaatccgtcc ctttttaaac tctacaggcc acggattacg tggcctgtag acgtcctaaa
     3781 aggtttaaaa gggaaaagga agaaaagggt ggaaacgcaa aaaacgcacc actacgtggc
     3841 cccgttgggg ccgcatttgt gcccctgaag gggcggggga ggcgtctggg caatccccgt
     3901 tttaccagtc ccctatcgcc gcctgagagg gcgcaggaag cgagtaatca gggtatcgag
     3961 gcggattcac ccttggcgtc caaccagcgg caccagcggc gcctgagagg ggcgcgccca
     4021 gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     4081 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
//
