LOCUS       40924_39383        4121 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA243X, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create 
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
  PUBMED    29074996
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden, 
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4121)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher 
            Weg 20b, Dresden 01627, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(411..427)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      706..800
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             932..1744
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1914..2022
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2116..2704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2761..3591)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3605..3956
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4029..4115)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggt ctcaacccac ctgcgatgac ccgtctctac
      301 ccgaagactt acccgatatc gcgattgtct tcgcgacgag acgcgacatc gcaggtgcga
      361 cgagaccaag gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc
      421 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      481 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      541 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
      601 tgcggtattt cacaccgcat atgatgcact ctcagtacaa tctgctctga tgccgcatag
      661 ttaagccagc cccgacaccc gccaacaccc gctgaactag tcttggactc ctgttgatag
      721 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
      781 cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat ttgtgtctca
      841 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      901 tgcttacata aacagtaata caaggggtgt tatgagccat attcagcgtg aaacgagctg
      961 tagccgtccg cgtctgaaca gcaacatgga tgcggatctg tatggctata aatgggcgcg
     1021 tgataacgtg ggtcagagcg gcgcgaccat ttatcgtctg tatggcaaac cggatgcgcc
     1081 ggaactgttt ctgaaacatg gcaaaggcag cgtggcgaac gatgtgaccg atgaaatggt
     1141 gcgtctgaac tggctgaccg aatttatgcc gctgccgacc attaaacatt ttattcgcac
     1201 cccggatgat gcgtggctgc tgaccaccgc gattccgggc aaaaccgcgt ttcaggtgct
     1261 ggaagaatat ccggatagcg gcgaaaacat tgtggatgcg ctggccgtgt ttctgcgtcg
     1321 tctgcatagc attccggtgt gcaactgccc gtttaacagc gatcgtgtgt ttcgtctggc
     1381 ccaggcgcag agccgtatga acaacggcct ggtggatgcg agcgattttg atgatgaacg
     1441 taacggctgg ccggtggaac aggtgtggaa agaaatgcat aaactgctgc cgtttagccc
     1501 ggatagcgtg gtgacccacg gcgattttag cctggataac ctgattttcg atgaaggcaa
     1561 actgattggc tgcattgatg tgggccgtgt gggcattgcg gatcgttatc aggatctggc
     1621 cattctgtgg aactgcctgg gcgaatttag cccgagcctg caaaaacgtc tgtttcagaa
     1681 atatggcatt gataatccgg atatgaacaa actgcaattt catctgatgc tggatgaatt
     1741 tttctaataa ttaattggac cgcggtccgc gcgttgtcct tttccgctgc ataaccctgc
     1801 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     1861 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     1921 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     1981 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccgataatct
     2041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2521 cacgcttccc gaagggagaa aggcggacag gcatccggta agcggcaggg tcggaacagg
     2581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2701 gaaaaacgcc agcaacgcgg ccgtgaaagg caggccggtc cgtggtggcc acggcctcta
     2761 ggccagatcc agcggcatct gggttagtcg agcgcgggcc gcttcccatg tctcaccagg
     2821 gcgagcctgt ttcgcgatct cagcatctga aatcttcccg gccttgcgct tcgctggggc
     2881 cttacccacc gccttggcgg gcttcttcgg tccaaaactg aacaacagat gtgtgacctt
     2941 gcgcccggtc tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt tgatctgcgt
     3001 cacggctgga tcaagcactc gcaacttgaa gtccttgatc gagggatacc ggccttccag
     3061 ttgaaaccac tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc tgtcccattg
     3121 catgagcagc tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca cgtcagccaa
     3181 ggcgtatttg gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt ctttggtgaa
     3241 cctgagttct acacggccct caccctcccg gtagatgatt gtttgcaccc agccggtaat
     3301 catcacactc ggtcttttcc ccttgccatt gggctcttgg gttaaccgga cttcccgccg
     3361 tttcaggcgc agggccgctt ctttgagctg gttgtaggaa gattcgatag ggacacccgc
     3421 catcgtcgct atgtcctccg ccgtcactga atacatcact tcatcggtga caggctcgct
     3481 cctcttcacc tggctaatac aggccagaac gatccgctgt tcctgaacac tgaggcgata
     3541 cgcggcctcg accagggcat tgcttttgta aaccattggg ggtgaggcca cgttcgacat
     3601 tccttgtgta taaggggaca ctgtatctgc gtcccacaat acaacaaatc cgtcccttta
     3661 caacaacaaa tccgtccctt cttaacaaca aatccgtccc ttaatggcaa caaatccgtc
     3721 cctttttaaa ctctacaggc cacggattac gtggcctgta gacgtcctaa aaggtttaaa
     3781 agggaaaagg aagaaaaggg tggaaacgca aaaaacgcac cactacgtgg ccccgttggg
     3841 gccgcatttg tgcccctgaa ggggcggggg aggcgtctgg gcaatccccg ttttaccagt
     3901 cccctatcgc cgcctgagag ggcgcaggaa gcgagtaatc agggtatcga ggcggattca
     3961 cccttggcgt ccaaccagcg gcaccagcgg cgcctgagag gggcgcgccc agctgtctag
     4021 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     4081 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
