LOCUS       40924_39378        4019 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA243, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 4019)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4019)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             830..1642
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1812..1920
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2014..2602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2659..3489)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3503..3854
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3927..4013)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttaaattt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      841 tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg cggatctgta
      901 tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt atcgtctgta
      961 tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg tggcgaacga
     1021 tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc tgccgaccat
     1081 taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga ttccgggcaa
     1141 aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg tggatgcgct
     1201 ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt ttaacagcga
     1261 tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg tggatgcgag
     1321 cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag aaatgcataa
     1381 actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc tggataacct
     1441 gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg gcattgcgga
     1501 tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc cgagcctgca
     1561 aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac tgcaatttca
     1621 tctgatgctg gatgaatttt tctaataatt aattggaccg cggtccgcgc gttgtccttt
     1681 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     1741 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     1801 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     1861 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     1921 taggccggcc gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggc atccggtaag
     2461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2581 aggggggcgg agcctatgga aaaacgccag caacgcggcc gtgaaaggca ggccggtccg
     2641 tggtggccac ggcctctagg ccagatccag cggcatctgg gttagtcgag cgcgggccgc
     2701 ttcccatgtc tcaccagggc gagcctgttt cgcgatctca gcatctgaaa tcttcccggc
     2761 cttgcgcttc gctggggcct tacccaccgc cttggcgggc ttcttcggtc caaaactgaa
     2821 caacagatgt gtgaccttgc gcccggtctt tcgctgcgcc cactccacct gtagcgggct
     2881 gtgctcgttg atctgcgtca cggctggatc aagcactcgc aacttgaagt ccttgatcga
     2941 gggataccgg ccttccagtt gaaaccactt tcgcagctgg tcaatttcta tttcgcgctg
     3001 gccgatgctg tcccattgca tgagcagctc gtaaagcctg atcgcgtggg tgctgtccat
     3061 cttggccacg tcagccaagg cgtatttggt gaactgtttg gtgagttccg tcaggtacgg
     3121 cagcatgtct ttggtgaacc tgagttctac acggccctca ccctcccggt agatgattgt
     3181 ttgcacccag ccggtaatca tcacactcgg tcttttcccc ttgccattgg gctcttgggt
     3241 taaccggact tcccgccgtt tcaggcgcag ggccgcttct ttgagctggt tgtaggaaga
     3301 ttcgataggg acacccgcca tcgtcgctat gtcctccgcc gtcactgaat acatcacttc
     3361 atcggtgaca ggctcgctcc tcttcacctg gctaatacag gccagaacga tccgctgttc
     3421 ctgaacactg aggcgatacg cggcctcgac cagggcattg cttttgtaaa ccattggggg
     3481 tgaggccacg ttcgacattc cttgtgtata aggggacact gtatctgcgt cccacaatac
     3541 aacaaatccg tccctttaca acaacaaatc cgtcccttct taacaacaaa tccgtccctt
     3601 aatggcaaca aatccgtccc tttttaaact ctacaggcca cggattacgt ggcctgtaga
     3661 cgtcctaaaa ggtttaaaag ggaaaaggaa gaaaagggtg gaaacgcaaa aaacgcacca
     3721 ctacgtggcc ccgttggggc cgcatttgtg cccctgaagg ggcgggggag gcgtctgggc
     3781 aatccccgtt ttaccagtcc cctatcgccg cctgagaggg cgcaggaagc gagtaatcag
     3841 ggtatcgagg cggattcacc cttggcgtcc aaccagcggc accagcggcg cctgagaggg
     3901 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3961 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
