LOCUS       40924_39368        3570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA241, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             381..1193
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1363..1471
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1565..2153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2210..3040)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3054..3405
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3478..3564)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
      301 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      361 acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc
      421 gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg
      481 gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc
      541 tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact
      601 ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg
      661 cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc
      721 cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca
      781 ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga
      841 gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc
      901 cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg
      961 tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct
     1021 gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga
     1081 actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg
     1141 ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat
     1201 taattggacc gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat
     1261 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
     1321 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
     1381 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
     1441 caacgggaat cctgctctgc gaggctggcc gtaggccggc cgataatctc atgaccaaaa
     1501 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1561 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1621 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1681 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1741 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1801 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1861 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1921 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1981 aagggagaaa ggcggacagg catccggtaa gcggcagggt cggaacagga gagcgcacga
     2041 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2101 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2161 gcaacgcggc cgtgaaaggc aggccggtcc gtggtggcca cggcctctag gccagatcca
     2221 gcggcatctg ggttagtcga gcgcgggccg cttcccatgt ctcaccaggg cgagcctgtt
     2281 tcgcgatctc agcatctgaa atcttcccgg ccttgcgctt cgctggggcc ttacccaccg
     2341 ccttggcggg cttcttcggt ccaaaactga acaacagatg tgtgaccttg cgcccggtct
     2401 ttcgctgcgc ccactccacc tgtagcgggc tgtgctcgtt gatctgcgtc acggctggat
     2461 caagcactcg caacttgaag tccttgatcg agggataccg gccttccagt tgaaaccact
     2521 ttcgcagctg gtcaatttct atttcgcgct ggccgatgct gtcccattgc atgagcagct
     2581 cgtaaagcct gatcgcgtgg gtgctgtcca tcttggccac gtcagccaag gcgtatttgg
     2641 tgaactgttt ggtgagttcc gtcaggtacg gcagcatgtc tttggtgaac ctgagttcta
     2701 cacggccctc accctcccgg tagatgattg tttgcaccca gccggtaatc atcacactcg
     2761 gtcttttccc cttgccattg ggctcttggg ttaaccggac ttcccgccgt ttcaggcgca
     2821 gggccgcttc tttgagctgg ttgtaggaag attcgatagg gacacccgcc atcgtcgcta
     2881 tgtcctccgc cgtcactgaa tacatcactt catcggtgac aggctcgctc ctcttcacct
     2941 ggctaataca ggccagaacg atccgctgtt cctgaacact gaggcgatac gcggcctcga
     3001 ccagggcatt gcttttgtaa accattgggg gtgaggccac gttcgacatt ccttgtgtat
     3061 aaggggacac tgtatctgcg tcccacaata caacaaatcc gtccctttac aacaacaaat
     3121 ccgtcccttc ttaacaacaa atccgtccct taatggcaac aaatccgtcc ctttttaaac
     3181 tctacaggcc acggattacg tggcctgtag acgtcctaaa aggtttaaaa gggaaaagga
     3241 agaaaagggt ggaaacgcaa aaaacgcacc actacgtggc cccgttgggg ccgcatttgt
     3301 gcccctgaag gggcggggga ggcgtctggg caatccccgt tttaccagtc ccctatcgcc
     3361 gcctgagagg gcgcaggaag cgagtaatca gggtatcgag gcggattcac ccttggcgtc
     3421 caaccagcgg caccagcggc gcctgagagg ggcgcgccca gctgtctagg gcggcggatt
     3481 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     3541 cgactgagcc tttcgtttta tttgatgcct
//
