LOCUS       40924_39343        3825 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA237C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3825)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..843
                     /codon_start=1
                     /label=ECFP
                     /note="enhanced CFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      857..951
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1083..1895
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2065..2173
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2187..2846)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2847..3618)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3733..3819)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      181 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
      361 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
      601 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      661 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      721 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      781 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      841 aagtaaacta gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg
      901 gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc
      961 cccaataatt acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata
     1021 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1081 ttatgagcca tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg
     1141 atgcggatct gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca
     1201 tttatcgtct gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca
     1261 gcgtggcgaa cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc
     1321 cgctgccgac cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg
     1381 cgattccggg caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca
     1441 ttgtggatgc gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc
     1501 cgtttaacag cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc
     1561 tggtggatgc gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga
     1621 aagaaatgca taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta
     1681 gcctggataa cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg
     1741 tgggcattgc ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta
     1801 gcccgagcct gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca
     1861 aactgcaatt tcatctgatg ctggatgaat ttttctaata attaattgga ccgcggtccg
     1921 cgcgttgtcc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt
     1981 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc
     2041 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg
     2101 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct
     2161 gcgaggctgg ccgtaggccg gccctaccgg cgcggcagcg ttacccgtgt cggcggctcc
     2221 aacggctcgc catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg
     2281 cgccgttccc attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg
     2341 ccgggctggc tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac
     2401 agggtcggga tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga
     2461 tcaaccacca cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac
     2521 gccacgcggt cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg
     2581 gagatccagc gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt
     2641 ccgatgagct tggaaagtgt cttctggctg accaccacgg cgttctggtg gcccatctgc
     2701 gccacgaggt gatgcagcag cattgccgcc gtgggtttcc tcgcaataag cccggcccac
     2761 gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg
     2821 ccgatttctc tggactgcgt ggccatgctt atctccatgc ggtaggggtg ccgcacggtt
     2881 gcggcaccat gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg
     2941 taaaagtggc agtcaattac agattttctt taacctacgc aatgagctat tgcggggggt
     3001 gccgcaatga gctgttgcgt accccccttt tttaagttgt tgatttttaa gtctttcgca
     3061 tttcgcccta tatctagttc tttggtgccc aaagaagggc acccctgcgg ggttccccca
     3121 cgccttcggc gcggctcccc ctccggcaaa aagtggcccc tccggggctt gttgatcgac
     3181 tgcgcggcct tcggccttgc ccaaggtggc gctgccccct tggaaccccc gcactcgccg
     3241 ccgtgaggct cggggggcag gcgggcgggc ttcgcccttc gactgccccc actcgcatag
     3301 gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg
     3361 acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc
     3421 ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag
     3481 ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt
     3541 gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc
     3601 gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg
     3661 cagcgaccgc gcagggcgaa gcccggagag caagcccgta gggggggcgc gcccagctgt
     3721 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3781 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcct
//
