LOCUS       40924_39318        3572 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA232, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3572)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(252..308)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             830..1642
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            1812..1920
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1934..2593)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2594..3365)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3480..3566)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc
      301 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttaaattt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      841 tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc aacatggatg cggatctgta
      901 tggctataaa tgggcgcgtg ataacgtggg tcagagcggc gcgaccattt atcgtctgta
      961 tggcaaaccg gatgcgccgg aactgtttct gaaacatggc aaaggcagcg tggcgaacga
     1021 tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa tttatgccgc tgccgaccat
     1081 taaacatttt attcgcaccc cggatgatgc gtggctgctg accaccgcga ttccgggcaa
     1141 aaccgcgttt caggtgctgg aagaatatcc ggatagcggc gaaaacattg tggatgcgct
     1201 ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc aactgcccgt ttaacagcga
     1261 tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac aacggcctgg tggatgcgag
     1321 cgattttgat gatgaacgta acggctggcc ggtggaacag gtgtggaaag aaatgcataa
     1381 actgctgccg tttagcccgg atagcgtggt gacccacggc gattttagcc tggataacct
     1441 gattttcgat gaaggcaaac tgattggctg cattgatgtg ggccgtgtgg gcattgcgga
     1501 tcgttatcag gatctggcca ttctgtggaa ctgcctgggc gaatttagcc cgagcctgca
     1561 aaaacgtctg tttcagaaat atggcattga taatccggat atgaacaaac tgcaatttca
     1621 tctgatgctg gatgaatttt tctaataatt aattggaccg cggtccgcgc gttgtccttt
     1681 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     1741 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     1801 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     1861 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     1921 taggccggcc ctaccggcgc ggcagcgtta cccgtgtcgg cggctccaac ggctcgccat
     1981 cgtccagaaa acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt
     2041 cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg
     2101 gcggctcctc gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc
     2161 ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg
     2221 cggcactgaa caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat
     2281 tgaccacgta ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct
     2341 cggccaccaa gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg
     2401 aaagtgtctt ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat
     2461 gcagcagcat tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt
     2521 tgcgttccgt ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg
     2581 actgcgtggc catgcttatc tccatgcggt aggggtgccg cacggttgcg gcaccatgcg
     2641 caatcagctg caacttttcg gcagcgcgac aacaattatg cgttgcgtaa aagtggcagt
     2701 caattacaga ttttctttaa cctacgcaat gagctattgc ggggggtgcc gcaatgagct
     2761 gttgcgtacc cccctttttt aagttgttga tttttaagtc tttcgcattt cgccctatat
     2821 ctagttcttt ggtgcccaaa gaagggcacc cctgcggggt tcccccacgc cttcggcgcg
     2881 gctccccctc cggcaaaaag tggcccctcc ggggcttgtt gatcgactgc gcggccttcg
     2941 gccttgccca aggtggcgct gcccccttgg aacccccgca ctcgccgccg tgaggctcgg
     3001 ggggcaggcg ggcgggcttc gcccttcgac tgcccccact cgcataggct tgggtcgttc
     3061 caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac
     3121 ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt tccccagaga
     3181 aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg gtgggtatgt
     3241 ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg caggcgcagc
     3301 ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag ccagccggtg
     3361 gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag cgaccgcgca
     3421 gggcgaagcc cggagagcaa gcccgtaggg ggggcgcgcc cagctgtcta gggcggcgga
     3481 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     3541 ttcgactgag cctttcgttt tatttgatgc ct
//
