LOCUS       40924_39283        9603 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA226, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 9603)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             127..1566
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1581..2501
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2553..3632
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3650..4630
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4812..5921
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      5935..6029
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             6161..6973
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7143..7251
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(7315..8460)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(8853..9384)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(9511..9597)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttaggaggaa
      121 aaacatatga ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa
      181 agtgatgatt tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg
      241 aatgactctc atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat
      301 aacattgtca attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga
      361 cgcaggacat acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag
      421 ctagaggcca attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta
      481 gaaaatgaac ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat
      541 gttcgggctt ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct
      601 gggatcatgt ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg
      661 tcaaccgatc cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat
      721 catccgataa cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc
      781 gcaaaagaaa ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt
      841 aattgggcgg tagagcacgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag
      901 agtctttgca ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat
      961 gatgtttgtt tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga
     1021 aatcattatg aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata
     1081 ttaccgaatg ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa
     1141 agcctgtttg ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag
     1201 tcaaatgcag gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc
     1261 gataatattg agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt
     1321 tttccttggg agtcatcatt caaatatcga gatgcgttag cattaaaagg tgcggaaagg
     1381 attgtagaag caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga
     1441 ccgttgcaac gattagtgac atatatttct catgaaaggc catctaacta tacggctaag
     1501 gatgttgcgg ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt
     1561 gtcccataat aggtaaaagt atggaaaatg aatcaaaata taaaaccatc gaccacgtta
     1621 tttgtgttga aggaaataaa aaaattcatg tttgggaaac gctgccagaa gaaaacagcc
     1681 caaagagaaa gaatgccatt attattgcgt ctggttttgc ccgcaggatg gatcattttg
     1741 ctggtctggc ggaatattta tcgcggaatg gatttcatgt gatccgctat gattcgcttc
     1801 accacgttgg attgagttca gggacaattg atgaatttac aatgtctata ggaaagcaga
     1861 gcttgttagc agtggttgat tggttaacta cacgaaaaat aaataacttc ggtatgttgg
     1921 cttccagctt atctgcgcgg atagcttatg caagcctatc tgaaatcaat gcttcgtttt
     1981 taatcaccgc agtcggtgtt gttaacttaa gatattctct tgaaagagct ttagggtttg
     2041 attatctcag tctacccatt aatgaattgc cggataatct ggattttgaa ggccataaat
     2101 tgggtgctga agtctttgcg agagattgtc ttgattttgg ttgggaagat ttagcttcta
     2161 caattaataa catgatgtat cttgatatac cgtttattgc ttttactgca aataacgata
     2221 attgggtcaa gcaagatgaa gttatcacat tgttatcaaa tattcgtagt aatcgatgca
     2281 agatatattc tttgttagga agttcgcatg acttgagtga aaatttagtg gtcctgcgca
     2341 atttttatca atcggttacg aaagccgcta tcgcgatgga taatgatcat ctggatattg
     2401 atgttgatat tactgaaccg tcatttgaac atttaactat tgcgacagtc aatgaacgcc
     2461 gaatgagaat tgagattgaa aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt
     2521 ctatcactca aatagcaata taaggactct ctatgaaatt tggaaacttt ttgcttacat
     2581 accaacctcc ccaattttct caaacagagg taatgaaacg tttggttaaa ttaggtcgca
     2641 tctctgagga gtgtggtttt gataccgtat ggttactgga gcatcatttc acggagtttg
     2701 gtttgcttgg taacccttat gtcgctgctg catatttact tggcgcgact aaaaaattga
     2761 atgtaggaac tgccgctatt gttcttccca cagcccatcc agtacgccaa cttgaagatg
     2821 tgaatttatt ggatcaaatg tcaaaaggac gatttcggtt tggtatttgc cgagggcttt
     2881 acaacaagga ctttcgcgta ttcggcacag atatgaataa cagtcgcgcc ttagcggaat
     2941 gctggtacgg gctgataaag aatggcatga cagagggata tatggaagct gataatgaac
     3001 atatcaagtt ccataaggta aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg
     3061 tttatgtggt ggctgaatca gcttcgacga ctgagtgggc tgctcaattt ggcctaccga
     3121 tgatattaag ttggattata aatactaacg aaaagaaagc acaacttgag ctttataatg
     3181 aagtggctca agaatatggg cacgatattc ataatatcga ccattgctta tcatatataa
     3241 catctgtaga tcatgactca attaaagcga aagagatttg ccggaaattt ctggggcatt
     3301 ggtatgattc ttatgtgaat gctacgacta tttttgatga ttcagaccaa acaagaggtt
     3361 atgatttcaa taaagggcag tggcgtgact ttgtattaaa aggacataaa gatactaatc
     3421 gccgtattga ttacagttac gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca
     3481 taattcaaaa agacattgat gctacaggaa tatcaaatat ttgttgtgga tttgaagcta
     3541 atggaacagt agacgaaatt attgcttcca tgaagctctt ccagtctgat gtcatgccat
     3601 ttcttaaaga aaaacaacgt tcgctattat attagctaag gagaaagaaa tgaaatttgg
     3661 attgttcttc cttaacttca tcaattcaac aactgttcaa gaacaaagta tagttcgtat
     3721 gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag tgtatgaaaa
     3781 tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg
     3841 tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc atcatcctgt
     3901 cgccatagcg gaggaagcgt gcttattgga tcagttaagt gaagggagat ttattttagg
     3961 gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg ttgaatatca
     4021 acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg
     4081 taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc atgcttatac
     4141 gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg agtgggcggc
     4201 caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta gatatgaata
     4261 tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag agatagacca
     4321 tcagttaatg atattagtta actataacga agatagtaat aaagctaaac aagagacgcg
     4381 tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg aaaataaact
     4441 tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa ctgcggctaa
     4501 gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac caatgaatga
     4561 tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaga agtaccacat
     4621 ggaatatacc taatagattt cgagttgcag cgaggcggca agtgaacgaa tccccaggag
     4681 catagataac tatgtgactg gggtgagtga aagcagccaa caaagcagca gcttgaaaga
     4741 tgaagggtat aaaagagtat gacagcagtg ctgccatact ttctaatatt atcttgagga
     4801 gtaaaacagg tatgacttca tatgttgata aacaagaaat tacagcaagc tcagaaattg
     4861 atgatttgat tttttcgagc gatccattag tgtggtctta cgacgagcag gaaaaaatca
     4921 gaaagaaact tgtgcttgat gcatttcgta atcattataa acattgtcga gaatatcgtc
     4981 actactgtca ggcacacaaa gtagatgaca atattacgga aattgatgac atacctgtat
     5041 tcccaacatc ggtttttaag tttactcgct tattaacttc tcaggaaaac gagattgaaa
     5101 gttggtttac cagtagcggc acgaatggtt taaaaagtca ggtggcgcgt gacagattaa
     5161 gtattgagag actcttaggc tctgtgagtt atggcatgaa atatgttggt agttggtttg
     5221 atcatcaaat agaattagtc aatttgggac cagatagatt taatgctcat aatatttggt
     5281 ttaaatatgt tatgagtttg gtggaattgt tatatcctac gacatttacc gtaacagaag
     5341 aacgaataga ttttgttaaa acattgaata gtcttgaacg aataaaaaat caagggaaag
     5401 atctttgtct tattggttcg ccatacttta tttatttact ctgccattat atgaaagata
     5461 aaaaaatctc attttctgga gataaaagcc tttatatcat aaccggaggc ggctggaaaa
     5521 gttacgaaaa agaatctctg aaacgtgatg atttcaatca tcttttattt gatactttca
     5581 atctcagtga tattagtcag atccgagata tatttaatca agttgaactc aacacttgtt
     5641 tctttgagga tgaaatgcag cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg
     5701 atcctgaaac gttgaaacct gtacctgatg gaacgccggg gttgatgagt tatatggatg
     5761 cgtcagcaac cagttatcca gcatttattg ttaccgatga tgtcgggata attagcagag
     5821 aatatggtaa gtatcccggc gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc
     5881 agaaagggtg tgctttgagc ttaaccgaag cgtttgatag ttgaactagt cttggactcc
     5941 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     6001 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     6061 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     6121 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcagcgtga
     6181 aacgagctgt agccgtccgc gtctgaacag caacatggat gcggatctgt atggctataa
     6241 atgggcgcgt gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc
     6301 ggatgcgccg gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga
     6361 tgaaatggtg cgtctgaact ggctgaccga atttatgccg ctgccgacca ttaaacattt
     6421 tattcgcacc ccggatgatg cgtggctgct gaccaccgcg attccgggca aaaccgcgtt
     6481 tcaggtgctg gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt
     6541 tctgcgtcgt ctgcatagca ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt
     6601 tcgtctggcc caggcgcaga gccgtatgaa caacggcctg gtggatgcga gcgattttga
     6661 tgatgaacgt aacggctggc cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc
     6721 gtttagcccg gatagcgtgg tgacccacgg cgattttagc ctggataacc tgattttcga
     6781 tgaaggcaaa ctgattggct gcattgatgt gggccgtgtg ggcattgcgg atcgttatca
     6841 ggatctggcc attctgtgga actgcctggg cgaatttagc ccgagcctgc aaaaacgtct
     6901 gtttcagaaa tatggcattg ataatccgga tatgaacaaa ctgcaatttc atctgatgct
     6961 ggatgaattt ttctaataat taattggacc gcggtccgcg cgttgtcctt ttccgctgca
     7021 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     7081 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     7141 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     7201 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc
     7261 cgcgatgcag gtggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
     7321 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
     7381 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
     7441 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
     7501 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
     7561 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
     7621 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
     7681 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
     7741 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
     7801 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
     7861 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
     7921 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
     7981 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
     8041 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
     8101 ggcctgcttg gcttcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
     8161 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
     8221 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
     8281 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg actatcgagc cgacggactg
     8341 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
     8401 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
     8461 tcaccctcct tgcgggattg ccccggaatt aattccccgg atcgatccgt cgatcttgat
     8521 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     8581 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     8641 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     8701 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     8761 tgcggactgg ctttctacgt ggctgccatt tttggggtga ggccgttcgc ggccgagggg
     8821 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg
     8881 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
     8941 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
     9001 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
     9061 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
     9121 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
     9181 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
     9241 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
     9301 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
     9361 gaggtatcca caacgccggc ggccctacat ggctctgctg tagtgagtgg gttgcgctcc
     9421 ggcagcggtc ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9481 tacggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     9541 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     9601 cct
//
