LOCUS       V016088                 7123 bp    DNA     circular SYN 07-DEC-2019
DEFINITION  Exported.
ACCESSION   V016088
VERSION     V016088
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Alvarez B, Mencia M, de Lorenzo V, Fernandez LA.
  TITLE     In vivo mutagenesis of target DNA in E. coli chromosome with
            processive fusions of base deaminases and T7 RNA polymerase
            terminated by RNA-guided dCas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Alvarez B, Fernandez LA.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2019) Microbial Biotechnology, CNB-CSIC (Centro
            Nacional de Biotecnologia-CSIC), Darwin 3, Cantoblanco, MADRID,
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 7123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2019) Microbial Biotechnology, CNB-CSIC (Centro Nacional de
            Biotecnologia-CSIC), Darwin 3, Cantoblanco, MADRID, Madrid 28049,
            Spain"
FEATURES             Location/Qualifiers
     source          1..7123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(32..118)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(164..787)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        803..858
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             913..3561
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     terminator      3575..3669
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             3801..4613
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            4783..4891
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(4955..6100)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(6493..7024)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 ggcgcgccga gctcgaattc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
       61 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
      121 gcctctagca cgcctgccag gaattgggga tcggagatct ttaagaccca ctttcacatt
      181 taagttgttt ttctaatccg catataatca attcaaggcc gaataagaag gctggctctg
      241 caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
      301 taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
      361 aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
      421 ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
      481 ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaga cttttagcgt
      541 tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
      601 tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
      661 acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
      721 ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctgg
      781 acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
      841 cctatcagtg atagagaaaa gtgaatctag agtcgacctg caggtttgca agcttaagaa
      901 ggagatatac atatgaacac gattaacatc gctaagaacg acttctctga catcgaactg
      961 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag
     1021 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag
     1081 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc
     1141 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc
     1201 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac
     1261 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct
     1321 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac
     1381 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac
     1441 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc
     1501 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc
     1561 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta
     1621 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc
     1681 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag
     1741 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg
     1801 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg
     1861 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg
     1921 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag
     1981 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg
     2041 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc
     2101 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc
     2161 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt
     2221 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt
     2281 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct
     2341 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca
     2401 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt
     2461 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt
     2521 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt
     2581 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag
     2641 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc
     2701 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg
     2761 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg
     2821 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag
     2881 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac
     2941 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg
     3001 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga
     3061 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg
     3121 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc
     3181 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct
     3241 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg
     3301 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc
     3361 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat
     3421 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct
     3481 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta
     3541 gagtcggact tcgcgttcgc gtaaactagt cttggactcc tgttgataga tccagtaatg
     3601 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     3661 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
     3721 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     3781 acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc
     3841 gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg
     3901 gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc
     3961 tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact
     4021 ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg
     4081 cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc
     4141 cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca
     4201 ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga
     4261 gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc
     4321 cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg
     4381 tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct
     4441 gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga
     4501 actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg
     4561 ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat
     4621 taattggacc gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat
     4681 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
     4741 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
     4801 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
     4861 caacgggaat cctgctctgc gaggctggcc gtaggccggc cgcgatgcag gtggctgctg
     4921 aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat
     4981 tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct
     5041 gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca
     5101 gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg
     5161 gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca
     5221 cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga
     5281 cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc
     5341 ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat
     5401 tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga
     5461 taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc
     5521 gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt
     5581 tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg
     5641 tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct
     5701 gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcttcgctga
     5761 cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg
     5821 tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct
     5881 ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga
     5941 tcttggccgt agcttgctgg actatcgagc cgacggactg gaaggtttcg cggggcgcac
     6001 gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca
     6061 gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg
     6121 ccccggaatt aattccccgg atcgatccgt cgatcttgat cccctgcgcc atcagatcct
     6181 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     6241 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     6301 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     6361 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
     6421 ggctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct ggggggatgg
     6481 gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc ttcggcgtgc
     6541 gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa
     6601 aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc
     6661 tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag
     6721 cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca
     6781 agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg
     6841 cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa
     6901 atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa
     6961 cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc
     7021 ggccctacat ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc ctgatccccc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tac
//
