LOCUS       40924_39253        5376 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA151, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 5376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        322..413
                     /label=AmpR promoter
     CDS             414..1271
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1278..1317
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1464..1572
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1586..2434)
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2424..3260)
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3774..4742)
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     promoter        complement(4766..4796)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     rep_origin      4852..5246
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(5284..5370)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca
      421 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg
      481 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg
      541 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac
      601 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg
      661 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat
      721 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg
      781 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc
      841 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt
      901 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag
      961 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc
     1021 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc
     1081 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca
     1141 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg
     1201 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga
     1261 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg cctcagcctg ccgccttggg ccgggtgatg tcgtacttgc
     1621 ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag ctccggcaac gcctcgcgca
     1681 cccgctggcg gcgcttgcgc atggtcgaac cactggcctc tgacggccag acatagccgc
     1741 acaaggtatc tatggaagcc ttgccggttt tgccggggtc gatccagcca cacagccgct
     1801 ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac ctcgtccatg ctgatgcgca
     1861 catgctggcc gccacccatg acggcctgcg cgatcaaggg gttcagggcc acgtacaggc
     1921 gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg aaacccctgc cgcttgcggc
     1981 cattctgggc gatgatggat accttccaaa ggcgctcgat gcagtcctgt atgtgcttga
     2041 gcgccccacc actatcgacc tctgccccga tttcctttgc cagcgcccga tagctacctt
     2101 tgaccacatg gcattcagcg gtgacggcct cccacttggg ttccaggaac agccggagct
     2161 gccgtccgcc ttcggtcttg ggttccgggc caagcactag gccattaggc ccagccatgg
     2221 ccaccagccc ttgcaggatg cgcagatcat cagcgcccag cggctccggg ccgctgaact
     2281 cgatccgctt gccgtcgccg tagtcatacg tcacgtccag cttgctgcgc ttgcgctcgc
     2341 cccgcttgag ggcacggaac aggccggggg ccagacagtg cgccgggtcg tgccggacgt
     2401 ggctgaggct gtgcttgttc ttaggcttca ccacggggca cccccttgct cttgcgctgc
     2461 ctctccagca cggcgggctt gagcaccccg ccgtcatgcc gcctgaacca ccgatcagcg
     2521 aacggtgcgc catagttggc cttgctcaca ccgaagcgga cgaagaaccg gcgctggtcg
     2581 tcgtccacac cccattcctc ggcctcggcg ctggtcatgc tcgacaggta ggactgccag
     2641 cggatgttat cgaccagtac cgagctgccc cggctggcct gctgctggtc gcctgcgccc
     2701 atcatggccg cgcccttgct ggcatggtgc aggaacacga tagagcaccc ggtatcggcg
     2761 gcgatggcct ccatgcgacc gatgacctgg gccatggggc cgctggcgtt ttcttcctcg
     2821 atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc ggccctcggc ggcgcgcttg
     2881 aggccgtcga accactccgg ggccatgatg ttgggcaggc tgccgatcag cggctggatc
     2941 agcaggccgt cagccacggc ttgccgttcc tcggcgctga ggtgcgcccc aagggcgtgc
     3001 aggcggtgat gaatggcggt gggcgggtct tcggcgggca ggtagatcac cgggccggtg
     3061 ggcagttcgc ccacctccag cagatccggc ccgcctgcaa tctgtgcggc cagttgcagg
     3121 gccagcatgg atttaccggc accaccgggc gacaccagcg ccccgaccgt accggccacc
     3181 atgttgggca aaacgtagtc cagcggtggc ggcgctgctg cgaacgcctc cagaatattg
     3241 ataggcttat gggtagccat tgattgcctc ctttgcaggc agttggtggt taggcgctgg
     3301 cggggtcact acccccgccc tgcgccgctc tgagttcttc caggcactcg cgcagcgcct
     3361 cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat ccctttggcc ttcatgcgct
     3421 cggcatatcg cgcttggcgt acagcgtcag ggctggccag caggtcgccg gtctgcttgt
     3481 ccttttggtc tttcatatca gtcaccgaga aacttgccgg ggccgaaagg cttgtcttcg
     3541 cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg ccatatcagc gactgaaaag
     3601 cggccagcct cggccttgtt tgacgtataa ccaaagccac cgggcaacca atagcccttg
     3661 tcacttttga tcaggtagac cgaccctgaa gcgctttttt cgtattccat aaaaccccct
     3721 tctgtgcgtg agtactcata gtataacagg cgtgagtacc aacgcaagca ctacatgctg
     3781 aaatctggcc cgcccctgtc catgcctcgc tggcggggtg ccggtgcccg tgccagctcg
     3841 gcccgcgcaa gctggacgct gggcagaccc atgaccttgc tgacggtgcg ctcgatgtaa
     3901 tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc tggcctcggc catggccttg
     3961 ccgatttcct cggcactgcg gccccggctg gccagcttct gcgcggcgat aaagtcgcac
     4021 ttgctgaggt catcaccgaa gcgcttgacc agcccggcca tctcgctgcg gtactcgtcc
     4081 agcgccgtgc gccggtggcg gctaagctgc cgctcgggca gttcgaggct ggccagcctg
     4141 cgggccttct cctgctgccg ctgggcctgc tcgatctgct ggccagcctg ctgcaccagc
     4201 gccgggccag cggtggcggt cttgcccttg gattcacgca gcagcaccca cggctgataa
     4261 ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg gccatagtgg
     4321 cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg ggcaatctgc
     4381 ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc atgcctgata gttcttcggg
     4441 ctggtttcca ctaccagggc aggctcccgg ccctcggctt tcatgtcatc caggtcaaac
     4501 tcgctgaggt cgtccaccag caccagacca tgccgctcct gctcggcggg cctgatatac
     4561 acgtcattgc cctgggcatt catccgcttg agccatggcg tgttctggag cacttcggcg
     4621 gctgaccatt cccggttcat catctggccg gtggtggcgt ccctgacgcc gatatcgaag
     4681 cgctcacagc ccatggcctt gagctgtcgg cctatggcct gcaaagtcct gtcgttcttc
     4741 atcgggccac caagcgattc ccacacatta tacgagccgg aagcataaag tgtaaagcct
     4801 agatccgaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
     4861 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctccagcg
     4921 tatttctgcg gggtttggtg tggggtttag cgggctttgc ccgcctttcc ccctgccgcg
     4981 cagcggtggg gcggtgtgta gcctagcgca gcgaatagac cagctatccg gcctctggcc
     5041 gggcatattg ggcaagggca gcagcgcccc acaagggcgc tgataaccgc gcctagtgga
     5101 ttattcttag ataatcatgg atggattttt ccaacacccc gccagccccc gcccctgctg
     5161 ggtttgcagg tttgggggcg tgacagttat tgcaggggtt cgtgacagtt attgcagggg
     5221 ggcgtgacag ttattgcagg ggttcgtgac agttagggcg cgcccagctg tctagggcgg
     5281 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     5341 gtctttcgac tgagcctttc gttttatttg atgcct
//
