LOCUS       40924_39238        3671 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA141, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3671)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        322..413
                     /label=AmpR promoter
     CDS             414..1271
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1278..1317
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1464..1572
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2311..3141)
                     /codon_start=1
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
                     /translation="VASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3155..3506
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(3579..3665)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca
      421 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg
      481 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg
      541 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac
      601 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg
      661 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat
      721 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg
      781 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc
      841 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt
      901 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag
      961 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc
     1021 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc
     1081 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca
     1141 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg
     1201 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga
     1261 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg ccgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gcatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg ccgtgaaagg
     2281 caggccggtc cgtggtggcc acggcctcta ggccagatcc agcggcatct gggttagtcg
     2341 agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt ttcgcgatct cagcatctga
     2401 aatcttcccg gccttgcgct tcgctggggc cttacccacc gccttggcgg gcttcttcgg
     2461 tccaaaactg aacaacagat gtgtgacctt gcgcccggtc tttcgctgcg cccactccac
     2521 ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga tcaagcactc gcaacttgaa
     2581 gtccttgatc gagggatacc ggccttccag ttgaaaccac tttcgcagct ggtcaatttc
     2641 tatttcgcgc tggccgatgc tgtcccattg catgagcagc tcgtaaagcc tgatcgcgtg
     2701 ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg gtgaactgtt tggtgagttc
     2761 cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct acacggccct caccctcccg
     2821 gtagatgatt gtttgcaccc agccggtaat catcacactc ggtcttttcc ccttgccatt
     2881 gggctcttgg gttaaccgga cttcccgccg tttcaggcgc agggccgctt ctttgagctg
     2941 gttgtaggaa gattcgatag ggacacccgc catcgtcgct atgtcctccg ccgtcactga
     3001 atacatcact tcatcggtga caggctcgct cctcttcacc tggctaatac aggccagaac
     3061 gatccgctgt tcctgaacac tgaggcgata cgcggcctcg accagggcat tgcttttgta
     3121 aaccattggg ggtgaggcca cgttcgacat tccttgtgta taaggggaca ctgtatctgc
     3181 gtcccacaat acaacaaatc cgtcccttta caacaacaaa tccgtccctt cttaacaaca
     3241 aatccgtccc ttaatggcaa caaatccgtc cctttttaaa ctctacaggc cacggattac
     3301 gtggcctgta gacgtcctaa aaggtttaaa agggaaaagg aagaaaaggg tggaaacgca
     3361 aaaaacgcac cactacgtgg ccccgttggg gccgcatttg tgcccctgaa ggggcggggg
     3421 aggcgtctgg gcaatccccg ttttaccagt cccctatcgc cgcctgagag ggcgcaggaa
     3481 gcgagtaatc agggtatcga ggcggattca cccttggcgt ccaaccagcg gcaccagcgg
     3541 cgcctgagag gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     3601 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     3661 atttgatgcc t
//
