LOCUS       40924_39233        3673 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA133, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3673)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3673)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..146
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        161..191
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    199..215
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    249..305
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..352)
                     /label=F24
                     /note="F24"
     terminator      604..698
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        771..862
                     /label=AmpR promoter
     CDS             863..1720
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1727..1766
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1913..2021
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2035..2694)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2695..3466)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3581..3667)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
      121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      241 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
      301 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      481 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      541 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgaactagtc
      601 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      661 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      721 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
      781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      841 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
      901 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
      961 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
     1021 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
     1081 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
     1141 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
     1201 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
     1261 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
     1321 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
     1381 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1441 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     1501 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     1561 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     1621 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     1681 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     1741 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     1801 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     1861 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     1921 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     1981 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cctaccggcg
     2041 cggcagcgtt acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa aacacggctc
     2101 atcgggcatc ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc
     2161 tggcaggtct ggttccatgc ccggaatgcc gggctggctg ggcggctcct cgccggggcc
     2221 ggtcggtagt tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt cgccatgccc
     2281 caacagcgat tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga acaccgacag
     2341 gcgcaactgg tcgcggggct ggccccacgc cacgcggtca ttgaccacgt aggccgacac
     2401 ggtgccgggg ccgttgagct tcacgacgga gatccagcgc tcggccacca agtccttgac
     2461 tgcgtattgg accgtccgca aagaacgtcc gatgagcttg gaaagtgtct tctggctgac
     2521 caccacggcg ttctggtggc ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt
     2581 gggtttcctc gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca
     2641 gtgaccgggc ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat
     2701 ctccatgcgg taggggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc
     2761 ggcagcgcga caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta
     2821 acctacgcaa tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt
     2881 taagttgttg atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa
     2941 agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa
     3001 gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc
     3061 tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
     3121 cgcccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag
     3181 ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag
     3241 cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg
     3301 ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc
     3361 ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca
     3421 gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc
     3481 cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca
     3541 agcccgtagg gggggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
     3601 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     3661 ttatttgatg cct
//
