LOCUS       40924_39223        3224 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA131, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3224)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        322..413
                     /label=AmpR promoter
     CDS             414..1271
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1278..1317
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1464..1572
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1586..2245)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2246..3017)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3132..3218)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca
      421 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg
      481 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg
      541 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac
      601 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg
      661 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat
      721 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg
      781 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc
      841 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt
      901 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag
      961 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc
     1021 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc
     1081 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca
     1141 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg
     1201 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga
     1261 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg ccctaccggc gcggcagcgt tacccgtgtc ggcggctcca
     1621 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc
     1681 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc
     1741 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca
     1801 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat
     1861 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg
     1921 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg
     1981 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc
     2041 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg
     2101 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg
     2161 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc
     2221 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc cgcacggttg
     2281 cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt
     2341 aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt gcggggggtg
     2401 ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag tctttcgcat
     2461 ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg gttcccccac
     2521 gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg ttgatcgact
     2581 gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg cactcgccgc
     2641 cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca ctcgcatagg
     2701 cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga
     2761 cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag gccggaggct
     2821 tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg cagttggagc
     2881 cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt caagctcgtg
     2941 ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc gagcgaagcg
     3001 agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc aagggagcgc
     3061 agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggggggcgcg cccagctgtc
     3121 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     3181 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcct
//
