LOCUS       40924_39213        3924 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSEVA121, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent 
            platform for analysis and deployment of complex prokaryotic 
            phenotypes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Silva-Rocha R, Martinez-Garcia E, Chavarria M, Calles B, 
            Arce-Rodriguez A, de las Heras A, Paez-Espino D, Durante-Rodriguez 
            G, Kim J, Nikel PI, Platero R, de Lorenzo V.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3924)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3924)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2012) Systems Biology Program, Centro Nacional de 
            Biotecnologia-CSIC, C/ Darwin 3, Campus de Cantoblanco, Madrid, 
            Madrid 28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: ApE v. v2.0.40
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..32
                     /label=R24
                     /note="R24"
     misc_feature    56..112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(121..144)
                     /label=F24
                     /note="F24"
     terminator      155..249
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        322..413
                     /label=AmpR promoter
     CDS             414..1271
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1278..1317
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1464..1572
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1636..2781)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(3174..3705)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(3832..3918)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg
      301 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca
      421 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg
      481 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg
      541 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac
      601 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg
      661 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat
      721 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg
      781 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc
      841 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt
      901 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag
      961 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc
     1021 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc
     1081 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca
     1141 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg
     1201 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga
     1261 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga
     1321 cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     1381 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     1441 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     1501 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     1561 cgaggctggc cgtaggccgg ccgcgatgca ggtggctgct gaacccccag ccggaactga
     1621 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
     1681 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
     1741 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
     1801 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
     1861 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
     1921 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
     1981 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
     2041 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
     2101 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
     2161 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
     2221 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
     2281 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
     2341 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
     2401 cttcgtgtgt ttcagcaacg cggcctgctt ggcttcgctg acctgttttg ccaggtcctc
     2461 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
     2521 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
     2581 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
     2641 gactatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
     2701 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
     2761 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccggaat taattccccg
     2821 gatcgatccg tcgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     2881 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     2941 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     3001 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     3061 atccggggtc agcaccgttt ctgcggactg gctttctacg tggctgccat ttttggggtg
     3121 aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc
     3181 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
     3241 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
     3301 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
     3361 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
     3421 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
     3481 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
     3541 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
     3601 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
     3661 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccctaca tggctctgct
     3721 gtagtgagtg ggttgcgctc cggcagcggt cctgatcccc cgcagaaaaa aaggatctca
     3781 agaagatcct ttgatctttt ctacggcgcg cccagctgtc tagggcggcg gatttgtcct
     3841 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
     3901 agcctttcgt tttatttgat gcct
//
