LOCUS       40924_39203        8498 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pSEVA-QSGEF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Zhang C, Parrello D, Brown PJ, Wall JD, Hu Z.
  TITLE     A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor 
            the expression of quorum sensing genes
  JOURNAL   Appl. Microbiol. Biotechnol. (2018) In press
  PUBMED    29730766
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Zhang C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8498)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8498)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2018) Department of Civil "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             437..1165
                     /codon_start=1
                     /label=TurboYFP
                     /note="enhanced derivative of PhiYFP"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYDFDAGSGDTS"
     CDS             complement(1449..2147)
                     /codon_start=1
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             3496..4203
                     /codon_start=1
                     /label=mNeonGreen
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     CDS             complement(4453..5127)
                     /codon_start=1
                     /label=DsRed-Express2
                     /note="noncytotoxic tetrameric variant of DsRed fluorescent
                     protein (Strack et al., 2008)"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKTIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     terminator      5530..5624
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             5756..6568
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            6738..6846
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(6860..7519)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(7520..8291)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(8406..8492)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaagt taaccaaaaa ggggggattt tatctcccct ttaatttttc ctatcgatag
       61 gcgactgacg aaacctcgct ccggcggggt tttttgttat ctgcatctag aaagtttcct
      121 ggctttcccg tcgggcggtg cgggtggcct ttgcccggaa ggccatgttt tggggctgtg
      181 ttctctcgtg tgaagccatt gctctgatct tttcggacgt ttcttcgagc ctagcaaggg
      241 tccgggttca ccgaaatcta tctcatttgc tagttataaa attatgaaat ttgcataaat
      301 tcttcagctt cctatttgga ggaagtgaag atgatcgtac aaattggtcg gcgcgaagag
      361 ttcgataaaa aactgctggg cgagatgcac aagttgcgtg ctcaagtgtt caaggagcgc
      421 ctaggaggaa aaacatatgt catccggcgc attgttattc catggcaaga tcccgtatgt
      481 ggtggaaatg gaaggtaacg ttgacggtca cacctttagc attcgcggga aaggttacgg
      541 agatgcctct gtcggtaaag tcgatgcgca gtttatctgc actacaggtg atgtcccagt
      601 accgtggtct acacttgtga ctaccttaac gtatggtgcc cagtgttttg cgaaatatgg
      661 ccctgaactg aaagacttct acaaatcgtg tatgcccgat ggctatgtgc aagaacggac
      721 cattaccttt gaaggcgatg gcaatttcaa gacccgtgcg gaagtgacct ttgagaatgg
      781 gagtgtgtat aaccgcgtta aactgaacgg acagggcttt aagaaagatg gccatgttct
      841 gggcaagaat ctggagttca actttacgcc gcattgcctg tacatttggg gcgatcaagc
      901 gaatcatggg ttgaaaagcg cctttaaaat ctgccatgaa attacaggta gcaaagggga
      961 tttcatcgta gctgaccata cccagatgaa cacgccgatt ggaggtggtc cagtccatgt
     1021 accggagtac caccacatgt cctaccacgt gaaactcagc aaagacgtga ctgaccaccg
     1081 tgacaacatg tcgctgaaag aaacggttcg tgctgttgac tgtcgcaaaa cctatgattt
     1141 cgatgcaggc tcaggtgata cgagttaatg aatattctcg gtaccaaatt ccagaaaaga
     1201 ggcctcccga aaggggggcc ttttttcgtt ttggtccaag cttccagaaa agacacccta
     1261 acgggtgttt tttcgttttt ggtctcccgg gaccgaaaca aaaaaagacg cttttcagcg
     1321 tcttcaataa ttggtttggt accgagtata cttgagaaga gaaaagaaaa ccgccgatcc
     1381 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     1441 attcattagt tcagcttgtg gcccagcttg ctcggcaggt cgcagtagcg ggccacggcc
     1501 acctcgtgct gctccacgta ggtttcgttg ttggcctcct tgatgcgctc caggcggtag
     1561 tccacgtagt acacgcccgg catcttcagg ttcttggccg gcttcttgct gcggtaggtg
     1621 gtcttggcgt tggcgatcag gtggctgccg cccaccagct tcagggccat gtcgttgcgg
     1681 ccctccaggc cgccgtcggc cgggtacagg gtttcggtga aggcctccca gcccagggtc
     1741 ttcttctgca tcaccgggcc gttgctggtg aagttcacgc cgcggatctt cacgttgtag
     1801 atcaggcagc cgtcctgcag gctggtgtcc tgggtggcgg tcagcacgcc gccgtcctcg
     1861 taggtggtca cgcgctccca ggtgaagccc tccgggaagc tctgcttgaa gaagtccggg
     1921 atgccctggg tgtggttgat gaaggtcttg ctgccgtaca ggaagctggt ggccaggatg
     1981 tcgaaggcga acggcagcgg gccgccctcc accaccttga tgcgcatggt ctgggtgccc
     2041 tcgtacggct tgccctcgcc ctcgctggtg cacttgaagt ggtggttgtc cacggtgccc
     2101 tccatgtaca gcttcatgtg catgttctcc ttgatcagct cgctcatatg tttttcctcc
     2161 tagtactcga tgaagacctg atgccggtag cgtcccagct cggcgatcat ggcggcggaa
     2221 agcccttcca gcgattcaga gagcaattcg atcatgacca agtccccgtg tcgtgccggc
     2281 cgagaaaaaa aaggcggcat ccctaccctg atactcctgg gccctctcag tcggaggaca
     2341 taccagcaca cacatgaggg ggaagactaa aggaggatga acggcaggca acctgccaga
     2401 tctggtaggg cgcgccgacc ggcgcgccct gcgcttcaga tgagacccag cgccgcggcg
     2461 taggcggcag ccagcgtctt gttcggcgcg tcgaacttct tctggatgtt cttgtggtgg
     2521 aagtttaaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt tttattgcgc
     2581 cggcgatcta actaaaaagg ccgctctgcg gccttttttc ttttcactgg cgccaccaat
     2641 gtaaaaaagc gccctaaagg cgcttttttg ctatctgcgg ccgccaaaag taaaaacccg
     2701 ccgaagcggg tttttacgta aaacaggtga aactgacgtc caaagaatcg cgaccagaaa
     2761 ggcatcgtcc ctggctacca gggtcagtgt cccgaccggc gattccattt tcctgtagct
     2821 atggggcatg taggtgtcct cttcggcagg ctcgcccagt gtactacgca atgggatttc
     2881 aacagggaag cctgcaaatg gcaggcgagg cggggcggag cgctatcggc ccgatggatg
     2941 gccgcctgct tccaggcatg ccgtcgcccc cttggagccc aggccgagcg cctcgaactg
     3001 tgagatttgg gaggcgattt gccgagcaaa gtgggttgtc attggtttgc catctcatgg
     3061 gttcggacga ggcctcgagc aagggttgta acggtttttg tctggccaat gggctcttgc
     3121 gtaaaaaggc tgccgccctt cttgcttggt tgccgttctc ggatcccgcg cagcccggtg
     3181 ggtgtgccaa atttctcgcg gtttggatcg cgccgattgc cgcggcctac gaagcccgtg
     3241 gttcttctcc ccgaaacttt ttcgttcgga ctccgaatat cgcgcttcgc ccagcgccgc
     3301 tagtttcccg ttcctgacaa agcaagcgct ctggctcagg tatctcctga tccggatgca
     3361 tatcgctgaa gagggaacgt tctgtcatgt ccacattggc caacctgacc gaggttctgt
     3421 tccgcctcga tttcgatccc gataccgccg tttatcacta tcggggccag actctcttcg
     3481 aaggaggaaa aacatatggt gagcaagggc gaggaggata acatggcctc tctcccagcg
     3541 acacatgagt tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag
     3601 ggcaccggca atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac
     3661 ctccagttct ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg
     3721 ccctaccctg acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa
     3781 gtccatcgca caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc
     3841 tacgagggaa gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac
     3901 ggtcctgtga tgaccaactc gctgaccgct gcggactggt gcaggtcgaa gaagacttac
     3961 cccaacgaca aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggcaag
     4021 cgctaccgga gcactgcgcg gaccacctac acctttgcca agccaatggc ggctaactat
     4081 ctgaagaacc agccgatgta cgtgttccgt aagacggagc tcaagcactc caagaccgag
     4141 ctcaacttca aggagtggca aaaggccttt accgatgtga tgggcatgga cgagctgtac
     4201 aagtaatgac ctgaggaaac acagaaaaaa gcccgcacct gacagtgcgg gctttttttt
     4261 tcgaccaaag gctgagctaa ggttgaaaaa taaaaacggc gctaaaaagc gccgtttttt
     4321 ttgacggtgg tacgcgtagt agaaatcatc cttagcgaaa gctaaggatt ttttttatct
     4381 gcgatcgcgg gagaccagaa acaaaaaaag gccccccgtt agggaggcct tcaataattg
     4441 gaccggttac tactggaaca ggtggtggcg ggcctcggcg cgctcgtact gctccaccac
     4501 ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggag tccacgtagt agtagccggg
     4561 cagcttcacg ggcttcttgg ccatgtagat tgtcttgaac tccaccaggt agtggccgcc
     4621 gcccttcagc ttcagcgcct tgtggatctc gcccttcagc acgccgtcgc gggggtacag
     4681 gcgctcggtg gagggctccc agcccagagt cttcttctgc attacggggc cgtcggaggg
     4741 gaagttcacg ccgatgaact tcacgtggta gatgaaggtg ccgtcctgca gggaggagtc
     4801 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
     4861 ctcggggaag gacagcttct tgtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
     4921 ggagccgtag gtgaactggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt
     4981 ggtcacctgc agcttggcgg tctgggtgcc ctcgtagggc ttgccctcgc cctcgccctc
     5041 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaaggg
     5101 cttgatgacg ttctcagtgc tatccatatg tttttcctcc tgctagctgc gcaacctccg
     5161 ggcgcactgc gcgtcccgcg gtcagcgcct tgcccgacga aaagctgcgt aacggtaagc
     5221 catgtcgctc ctgcatcgct ctcccccttc cgaggctggg caccgagcga aaaactcttc
     5281 acgaaatggc gacgagttac gagctaatac aattgatagg gtggtgacag gtcaggccga
     5341 atggctttcc gcgaggatcg ggtacagcga cgcgaccaac agcagcgcca tccctacgtt
     5401 gaacaccctc agccagcgcg gtcgactgca gacgtaaaaa aagcggcgtg gttagccgct
     5461 tttttaattg ccggatatca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     5521 ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     5581 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata
     5641 attacgattt aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     5701 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     5761 ccatattcag cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca tggatgcgga
     5821 tctgtatggc tataaatggg cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg
     5881 tctgtatggc aaaccggatg cgccggaact gtttctgaaa catggcaaag gcagcgtggc
     5941 gaacgatgtg accgatgaaa tggtgcgtct gaactggctg accgaattta tgccgctgcc
     6001 gaccattaaa cattttattc gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc
     6061 gggcaaaacc gcgtttcagg tgctggaaga atatccggat agcggcgaaa acattgtgga
     6121 tgcgctggcc gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa
     6181 cagcgatcgt gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg gcctggtgga
     6241 tgcgagcgat tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat
     6301 gcataaactg ctgccgttta gcccggatag cgtggtgacc cacggcgatt ttagcctgga
     6361 taacctgatt ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat
     6421 tgcggatcgt tatcaggatc tggccattct gtggaactgc ctgggcgaat ttagcccgag
     6481 cctgcaaaaa cgtctgtttc agaaatatgg cattgataat ccggatatga acaaactgca
     6541 atttcatctg atgctggatg aatttttcta ataattaatt ggaccgcggt ccgcgcgttg
     6601 tccttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     6661 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     6721 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     6781 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     6841 tggccgtagg ccggccctac cggcgcggca gcgttacccg tgtcggcggc tccaacggct
     6901 cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag gcgctgctgc ccgcgccgtt
     6961 cccattcctc cgtttcggtc aaggctggca ggtctggttc catgcccgga atgccgggct
     7021 ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg ctcgcccgga tacagggtcg
     7081 ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc ctggtcgtcg tgatcaacca
     7141 ccacggcggc actgaacacc gacaggcgca actggtcgcg gggctggccc cacgccacgc
     7201 ggtcattgac cacgtaggcc gacacggtgc cggggccgtt gagcttcacg acggagatcc
     7261 agcgctcggc caccaagtcc ttgactgcgt attggaccgt ccgcaaagaa cgtccgatga
     7321 gcttggaaag tgtcttctgg ctgaccacca cggcgttctg gtggcccatc tgcgccacga
     7381 ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat aagcccggcc cacgcctcat
     7441 gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt cttggcttga atgccgattt
     7501 ctctggactg cgtggccatg cttatctcca tgcggtaggg gtgccgcacg gttgcggcac
     7561 catgcgcaat cagctgcaac ttttcggcag cgcgacaaca attatgcgtt gcgtaaaagt
     7621 ggcagtcaat tacagatttt ctttaaccta cgcaatgagc tattgcgggg ggtgccgcaa
     7681 tgagctgttg cgtacccccc ttttttaagt tgttgatttt taagtctttc gcatttcgcc
     7741 ctatatctag ttctttggtg cccaaagaag ggcacccctg cggggttccc ccacgccttc
     7801 ggcgcggctc cccctccggc aaaaagtggc ccctccgggg cttgttgatc gactgcgcgg
     7861 ccttcggcct tgcccaaggt ggcgctgccc ccttggaacc cccgcactcg ccgccgtgag
     7921 gctcgggggg caggcgggcg ggcttcgccc ttcgactgcc cccactcgca taggcttggg
     7981 tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc ttgacccgcc
     8041 ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga ggcttttccc
     8101 cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg gagccggtgg
     8161 gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct cgtgggcagg
     8221 cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga agcgagccag
     8281 ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga gcgcagcgac
     8341 cgcgcagggc gaagcccgga gagcaagccc gtaggggggg cgcgcccagc tgtctagggc
     8401 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     8461 cagtctttcg actgagcctt tcgttttatt tgatgcct
//
