LOCUS       40924_39188        4074 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pSEVA-Plac-mCherry, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Zhang C, Parrello D, Brown PJ, Wall JD, Hu Z.
  TITLE     A novel whole-cell biosensor of Pseudomonas aeruginosa to monitor 
            the expression of quorum sensing genes
  JOURNAL   Appl. Microbiol. Biotechnol. (2018) In press
  PUBMED    29730766
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Zhang C.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4074)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4074)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2018) Department of Civil "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..4074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    282..298
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    315..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             385..1092
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      1106..1200
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1332..2144
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2314..2422
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2436..3095)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(3096..3867)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(3982..4068)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt accatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      241 tcgtcttcac ctcgagaaaa tttatcaaaa agagtgttga cttgtgagcg gataacaatg
      301 atacttagat tcaattgtga gcggataaca atttcacaca tctagagtcg acctgcaggc
      361 atgcaagctt aggaggaaaa acatatggtg agcaagggcg aggaggataa catggccatc
      421 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      481 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      541 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      601 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      661 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      721 acccaggact cctccctgca agacggcgag ttcatctaca aggtgaagct gcgcggcacc
      781 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      841 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      901 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      961 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1021 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1081 gagctgtaca agtaaactag tcttggactc ctgttgatag atccagtaat gacctcagaa
     1141 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat
     1201 ccaggggtcc ccaataatta cgatttaaat ttgtgtctca aaatctctga tgttacattg
     1261 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     1321 caaggggtgt tatgagccat attcagcgtg aaacgagctg tagccgtccg cgtctgaaca
     1381 gcaacatgga tgcggatctg tatggctata aatgggcgcg tgataacgtg ggtcagagcg
     1441 gcgcgaccat ttatcgtctg tatggcaaac cggatgcgcc ggaactgttt ctgaaacatg
     1501 gcaaaggcag cgtggcgaac gatgtgaccg atgaaatggt gcgtctgaac tggctgaccg
     1561 aatttatgcc gctgccgacc attaaacatt ttattcgcac cccggatgat gcgtggctgc
     1621 tgaccaccgc gattccgggc aaaaccgcgt ttcaggtgct ggaagaatat ccggatagcg
     1681 gcgaaaacat tgtggatgcg ctggccgtgt ttctgcgtcg tctgcatagc attccggtgt
     1741 gcaactgccc gtttaacagc gatcgtgtgt ttcgtctggc ccaggcgcag agccgtatga
     1801 acaacggcct ggtggatgcg agcgattttg atgatgaacg taacggctgg ccggtggaac
     1861 aggtgtggaa agaaatgcat aaactgctgc cgtttagccc ggatagcgtg gtgacccacg
     1921 gcgattttag cctggataac ctgattttcg atgaaggcaa actgattggc tgcattgatg
     1981 tgggccgtgt gggcattgcg gatcgttatc aggatctggc cattctgtgg aactgcctgg
     2041 gcgaatttag cccgagcctg caaaaacgtc tgtttcagaa atatggcatt gataatccgg
     2101 atatgaacaa actgcaattt catctgatgc tggatgaatt tttctaataa ttaattggac
     2161 cgcggtccgc gcgttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2221 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2281 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2341 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2401 tcctgctctg cgaggctggc cgtaggccgg ccctaccggc gcggcagcgt tacccgtgtc
     2461 ggcggctcca acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc
     2521 tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg
     2581 cccggaatgc cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg
     2641 cccggataca gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg
     2701 tcgtcgtgat caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc
     2761 tggccccacg ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc
     2821 ttcacgacgg agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc
     2881 aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg
     2941 cccatctgcg ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc
     3001 ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg
     3061 gcttgaatgc cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc
     3121 cgcacggttg cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta
     3181 tgcgttgcgt aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt
     3241 gcggggggtg ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag
     3301 tctttcgcat ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg
     3361 gttcccccac gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg
     3421 ttgatcgact gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg
     3481 cactcgccgc cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca
     3541 ctcgcatagg cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg
     3601 cgagccttga cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag
     3661 gccggaggct tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg
     3721 cagttggagc cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt
     3781 caagctcgtg ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc
     3841 gagcgaagcg agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc
     3901 aagggagcgc agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggggggcgcg
     3961 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4021 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcct
//
