LOCUS       62056_19470        8824 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(115..236)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        481..838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             889..1821
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1873..2661
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2728..2776
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        2926..3030
                     /label=AmpR promoter
     CDS             3031..3888
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(4049..4637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4753..5036
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     CDS             7119..8768
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
       61 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
      121 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      181 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      241 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
      301 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
      361 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
      421 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
      481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      841 gcttgattct tctgacacaa cagtctcgaa ccaaaggctg gagccaccat ggcttccaag
      901 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
      961 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     1021 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     1081 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     1141 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     1201 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     1261 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     1321 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     1381 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     1441 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     1501 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     1561 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     1621 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     1681 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     1741 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     1801 cgcgtgctga agaacgagca gaccggtggt gggagcggag gtggcggatc aggtggcgga
     1861 ggctccggag ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2701 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2761 gttggttttt tgtgtgaatc gatagcgata atctttgcgc ttgcgttttc ccttgtccag
     2821 atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac
     2881 gtaatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2941 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3001 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3061 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3121 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3181 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttc
     3241 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3301 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3361 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3421 actgcggcca acttacttct gacaactatc ggaggaccga aggagctaac cgcttttttg
     3481 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3541 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3601 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3661 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3721 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3781 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3841 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta attcgaaatg
     3901 accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca
     3961 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4021 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4081 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4141 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4201 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4261 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4321 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4381 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4441 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4501 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4561 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4621 gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4681 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4741 tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct gggatggtga
     4801 aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct gtaccctctg
     4861 gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag tttttcgggt
     4921 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt tttagcggtg
     4981 cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc catcgagata
     5041 aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatctc gagctcaagc
     5101 ttcgaattct gtgaatcatt tatatcaggt cctgatcatc tctgacaata gtatttagtc
     5161 ctcaacaaaa acaaagccag aacttgtctc cctaaatatt gtcctgggaa ggactataca
     5221 tctcaaacca gtctttccta caactgcttc tcaaattcta gtgagcaaac aaattacctg
     5281 gagttctcat taaaattcag attctggtgt tctctggtgg tgcagtgggt taaggatgca
     5341 gcattgtcac tgctgtggca ctggttttat ccctggtcct ggaacttctg tatgccatgg
     5401 acacagccaa aaaaaaaaaa aaaaaaaaaa ttcagattct ggttcaggac ccacttggtg
     5461 ggaccagaga tttaggatct ctaacaggct ccctgattgt aatttagtag taagtcctct
     5521 gaagacactt cctgagtccc actgctgatc tactggactg attgcttcag cttttcagag
     5581 caatgaagaa atcaggctgg aataagatgc tccaaaacct cctgcttctc ttcaacaccc
     5641 cccattcctc atttccaccc ccagcccacc cacacacaca tcagaaaact aagagaattt
     5701 aactaacaga aaaataatca gggcagagca aagatttcct tcaatcatta gatataacgg
     5761 ggcaccatat gtcagatgct aggggaaaca aagatgatta agacaggacc ttagccttga
     5821 gttgatcaca aaccagcaga gaaaacacac atagataact cacaagtgga aaggaactaa
     5881 gataaaagaa ccacagaggg aggagttaac tctgtaggga aagagtgatg gtctagaaaa
     5941 gtgattcttt tggggttttt ttagggctgc acccatggca tagagaagtt ccccggctag
     6001 gggttcaact ggagctacag ctgccagctt ccaccacagc cacagcagcg ccagatctga
     6061 gccacatttg tgacctgcac cacaactcac aaagccagat acctaaccca ctgagcgagg
     6121 ccagggatca aacccacttc ctcatggata ctagtcgggt tcattactgc tgagccatgt
     6181 cgggaactcc aacactgatt tttttatttt attttatttt atttgtcttt ttgtcttttc
     6241 cagggccaca ccggcaacat atggatgtcc ccaggctagg ggtctaatca gggctgtagc
     6301 tgctgtccta caccagagcc acagcaatgc tagatccaag ctgcgtctga gacctacacc
     6361 acagctcacc acaatgtggg atccttaacc cactgagtgg ggccagggat caaacccgaa
     6421 gtttcatggt tcttagttgg attcgttaac cactgagcca ctacgggaac tccccaggtt
     6481 tatatatttt tttaatgaag ttaggtgcag ttagtgtaaa gatagctggg cagattaaag
     6541 caatgacgag attccgtggt agtcaacagt gctgattcat caaatacatc tggttctccc
     6601 tccctggagt gaatgttctt cctggcccct ctgtgtttgg gtagggcctt atgactcatt
     6661 tccaaccaat gagttgtaaa ttaaagtgcc cagagtcact ttaattgcca gcgcgagacc
     6721 ctctagcgtt ctctcattat ggcagggcaa ctcatccgcc tgggtttcta agtagctcca
     6781 cagcgacccc agctgcccca gctggattcc taacccagga ttcttaacac attgagcctc
     6841 agggggaact ccattttaat gtttattgct gagtgatttg tagtggctaa aaactgaaaa
     6901 ctagatttca aaatatgggt tattatgtca tattatgaaa tattgtgcaa ctgccaaaaa
     6961 gacaactttt catccttttt ggctcttggc aatactgcca ccacttggag tggtggaaga
     7021 ggcggagctt ctgcaatgca ccccaggcca cgcctacttt agccagactc ttctcacgtg
     7081 tgctccggga ggctccgctt tgacgcatgg atcccaccat ggaagatgcc aaaaacatta
     7141 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     7201 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     7261 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     7321 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     7381 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     7441 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     7501 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     7561 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     7621 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     7681 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     7741 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     7801 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     7861 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     7921 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     7981 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     8041 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     8101 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     8161 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     8221 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     8281 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     8341 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     8401 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     8461 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     8521 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     8581 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     8641 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     8701 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     8761 tcgccgtgta attctagttg tttaaacgag ctcgctagcc tcgagtctag agtcgacctg
     8821 cagg
//
