LOCUS       pSELECT-zeo-Luci        3769 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for the expression of secreted Lucia(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSELECT-zeo-Lucia
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   InvivoGen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    9..57
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    68..159
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        196..407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             420..688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             728..1357
                     /codon_start=1
                     /product="secreted coelenterazine-utilizing luciferase"
                     /label=secreted coelenterazine-utilizing luciferase
                     /note="Lucia(R)"
                     /note="synthetic gene based on luciferases from marine
                     copepods"
                     /translation="MEIKVLFALICIAVAEAKPTEINEDLNIAAVASNFATTDLETDLF
                     TNWETMNVISTDTEQVNTDADRGKLPGKKLPPDVLRELEANARRAGCTRGCLICLSHIK
                     CTPKMKKFIPGRCHTYEGEKESAQGGIGEAIVDIPEIPGFKDKEPLDQFIAQVDLCADC
                     TTGCLKGLANVQCSDLLKKWLPQRCTTFASKIQGRVDKIKGLAGDR"
     polyA_signal    complement(1379..1500)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1600..1994)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2011..2382)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(2402..2449)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(2495..2698)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2699..3002)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
       61 cgtaactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
      121 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgaaggatc tgcgatcgct
      181 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      241 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      301 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      361 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
      421 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
      481 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
      541 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
      601 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
      661 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacc tgagatcacc
      721 ggtcaccatg gaaatcaagg tgctgtttgc cctcatctgt attgctgttg ctgaggcaaa
      781 acccactgaa atcaatgaag acctcaatat agctgctgtg gcctccaact ttgccaccac
      841 agatcttgag actgacctgt tcaccaactg ggagaccatg aatgtgatta gcactgacac
      901 agagcaggtg aacacagatg ctgacagggg caagctgcct ggcaaaaaac tccccccaga
      961 tgtcctgagg gagctggagg ccaatgccag aagggctggt tgcacaagag gctgcctcat
     1021 ttgcctctcc cacattaagt gcacccctaa gatgaagaaa tttatccctg gcaggtgcca
     1081 cacttatgaa ggtgaaaagg agtctgctca gggagggatt ggagaggcaa ttgttgatat
     1141 cccagagatt cctggcttca aggataagga gccactggac cagtttattg ctcaagtgga
     1201 cctctgtgct gattgcacca ctggctgtct gaagggcctt gccaatgtcc agtgctctga
     1261 cctcctgaag aagtggcttc cccagaggtg taccactttt gccagcaaga ttcagggtag
     1321 ggtggacaaa atcaagggtc tggctgggga cagatgatag ctagctggcc agacatgata
     1381 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1441 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1501 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1561 taaagcaagt aaaacctcta caaatgtggt atggaattct aaaatacagc atagcaaaac
     1621 tttaacctcc aaatcaagcc tctacttgaa tccttttctg agggatgaat aaggcatagg
     1681 catcaggggc tgttgccaat gtgcattagc tgtttgcagc ctcaccttct ttcatggagt
     1741 ttaagatata gtgtattttc ccaaggtttg aactagctct tcatttcttt atgttttaaa
     1801 tgcactgacc tcccacattc cctttttagt aaaatattca gaaataattt aaatacatca
     1861 ttgcaatgaa aataaatgtt ttttattagg cagaatccag atgctcaagg cccttcataa
     1921 tatcccccag tttagtagtt ggacttaggg aacaaaggaa cctttaatag aaattggaca
     1981 gcaagaaagc gagcttctag cttatcctca gtcctgctcc tctgccacaa agtgcacgca
     2041 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     2101 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     2161 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     2221 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     2281 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     2341 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atgatggccc tcctatagtg
     2401 agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaaa cagcgtggat
     2461 ggcgtctcca gcttatctga cggttcacta aacgagctct gcttatatag acctcccacc
     2521 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2581 cccgttgatt tactagtcaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2641 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcatca
     2701 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2761 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2821 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2881 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2941 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     3001 cgcctgcagg ttaattaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3721 ctcacgttaa gggattttgg tcatggctag ttaattaaca tttaaatca
//
