LOCUS       40924_39108        6513 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPEhBCTNf2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6513)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6513)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..357
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    358..496
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     CDS             512..2017
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             2551..2625
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88324.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2627..2692
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2822..2842
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3267..3401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3406..3641)
                     /label=SV40 early enhancer
                     /note="SV40 early enhancer"
                     /regulatory_class="promoter"
     rep_origin      complement(3971..4559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4733..5590)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5591..5695)
                     /label=AmpR promoter
     rep_origin      5722..6177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     regulatory      complement(6345..6441)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      6345..6441
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    6367..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg
       61 ggggcccggc ctccccgatg cagaccacag cgccctcacg ggctgccctc aggccgcgca
      121 gcgggcagcc gccagccgtc accccgggga gcgtccgtgg ggtgcccagg caccccaccc
      181 cggcccgggg cgctcagacg gcagcagact gctgggcggc gcggggacta ctttccaccg
      241 ccccctcgcg ccccgcccct tgtcctcgcg cggcggaacg ctccgcgctg cgccggtggc
      301 ggcaggatac agcggcttct gcgcgactta taagagctcc ttgtgcggcg ccattttaag
      361 cctctcggtc tgtggcagca gcgttggccc ggccccggga gcggagagcg aggggaggcg
      421 gagacggagg aaggtctgag gagcagcttc agtccccgcc gagccgccac cgcaggtcga
      481 ggacggtcgg actcccgacc tcgaagcttc ctgcatgctg ctgctgctgc tgctgctggg
      541 cctgaggcta cagctctccc tgggcatcat cccagttgag gaggagaacc cggacttctg
      601 gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc
      661 cgccaagaac ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc
      721 caggatccta aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga
      781 ccgcttccca tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag
      841 tggagccaca gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt
      901 gagtgcagcc gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt
      961 gatgaatcgg gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca
     1021 gcacgcctcg ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc
     1081 cgacgtgcct gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc
     1141 caacatggac attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac
     1201 cccagaccct gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa
     1261 tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga
     1321 gctcatgcag gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg
     1381 agacatgaaa tacgagatcc accgagactc cacactggac ccctccctga tggagatgac
     1441 agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg
     1501 tggtcgcatc gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat
     1561 catgttcgac gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag
     1621 cctcgtcact gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag
     1681 ctccatcttc gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct
     1741 atacggaaac ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag
     1801 cgagagcggg agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca
     1861 cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt
     1921 gcaggagcag accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac
     1981 cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gttaacccgt
     2041 ggtccccgcg ttgcttcctc tgctggccgg gacatcaggt ggcccccgct gaattggaat
     2101 cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg
     2161 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     2221 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     2281 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     2341 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat
     2401 tcagcgatga cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta
     2461 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
     2521 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
     2581 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     2641 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     2701 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     2761 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     2821 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     2881 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     2941 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     3001 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     3061 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     3121 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     3181 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3241 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3361 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgaaccatg gagcggagaa
     3421 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     3481 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3541 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3601 cctggggact ttccacaccc taactgacac acattccaca gcagatcctc tagggatccg
     3661 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     3721 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     3781 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4381 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     4441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5701 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5761 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5821 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5881 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5941 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6001 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6061 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6121 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc
     6181 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6241 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgtgga ggttttactt
     6301 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6361 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6481 gtatcttatg gtactgtaac tgagctaaca taa
//
