LOCUS       40924_39103        6210 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPEhBCTNf1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6210)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..162
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    163..201
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     CDS             209..1714
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             2248..2322
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88330.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2324..2389
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2519..2539
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2964..3098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3103..3338)
                     /label=SV40 early enhancer
                     /note="SV40 early enhancer"
                     /regulatory_class="promoter"
     rep_origin      complement(3668..4256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4430..5287)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5288..5392)
                     /label=AmpR promoter
     rep_origin      5419..5874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     regulatory      complement(6042..6138)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      6042..6138
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    6064..6185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg
       61 ccccttgtcc tcgcgcggcg gaacgctccg cgctgcgccg gtggcggcag gatacagcgg
      121 cttctgcgcg acttataaga gctccttgtg cggcgccatt ttaagcctct cggtctgtgg
      181 cagcagcgtt ggcccggccc cagcttcctg catgctgctg ctgctgctgc tgctgggcct
      241 gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa
      301 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
      361 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
      421 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
      481 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
      541 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
      601 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
      661 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
      721 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
      781 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
      841 catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc
      901 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
      961 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct
     1021 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga
     1081 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     1141 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     1201 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     1261 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     1321 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     1381 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     1441 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     1501 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     1561 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     1621 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     1681 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt
     1741 ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga
     1801 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
     1861 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     1921 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     1981 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     2041 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca
     2101 gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt
     2161 tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat
     2221 aagcggatga atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt
     2281 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     2341 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     2401 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     2461 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     2521 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     2581 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     2641 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     2701 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     2761 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     2821 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     2881 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2941 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttatcatg tctggatctg aaccatggag cggagaatgg
     3121 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     3181 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     3241 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     3301 ggggactttc cacaccctaa ctgacacaca ttccacagca gatcctctag ggatccgtcg
     3361 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3421 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3481 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4081 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     4141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5281 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5401 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5461 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5521 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5581 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5641 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5701 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5761 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5821 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt
     5881 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5941 gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct
     6001 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6061 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6181 tcttatggta ctgtaactga gctaacataa
//
