LOCUS       40924_39098       11420 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPBhBCTNf3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 11420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..3504
                     /label=5' flanking region of human BCTN
                     /note="5' flanking region of human BCTN"
     regulatory      3505..4499
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    4500..4665
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     misc_feature    4666..5668
                     /label=part of intron 1 of human BCTN
                     /note="part of intron 1 of human BCTN"
     CDS             5667..7181
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             7715..7789
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88318.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          7791..7856
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7986..8006
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8431..8565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8878..9466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9640..10497)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10498..10602)
                     /label=AmpR promoter
     rep_origin      10629..11084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    11274..11395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattctgtc
       61 tttggtttgt tcatgctttt cagtctgcac agcagggatg ttcttaagaa gttgccctct
      121 gcgaaatttc actaaatggg aaccgtggac cctgcatgat gggattataa tcacaccagc
      181 cactcctttt acataatttt ttgtttaaga tccagtaaga ctccctgttt ttaatacata
      241 taaagcaaca gacatgtata tgtcgataaa ctgagggttt gcgtccctat tgcctggtgc
      301 aagctacagc aaaaaaccgg tgatatcaca tctacaaatc tcaatggatt caaaaacaat
      361 atgaaaattt ttagctgaca agaacatgtc tgctgcctgc agtccctgca atagctcacc
      421 atgttgccta ctaaccaaaa gatcaaaaat tatcgatttc atggcgctgc gaggtctgga
      481 aagaaaagct actttaaaat ttgcattgtt taagaaatga cttaaaagaa aagaatcatt
      541 tttttttctg aatactttgg aaggtgaaaa tggcttgttt taatcttatc catgatttac
      601 tataattcta ttatttttag cagctggagt ccagaaatga ttttttcgat gaatttagga
      661 ttttgccgca gtatacatct gcagtcccag aatcctccgg taagttaatc cttcttgcga
      721 tgggaaacgc ctttaacctt cttgtgaatg aaatatctgc aaccattttg aaatttttaa
      781 tggggtgtgt gtgtgtgtgc acgtgcgtgt gttgcgggtg tgtgtctccg aaacatgatt
      841 tcttagaaat gcagcggtac ttgtctgagt tcttagaaac attgagaaac atatatacct
      901 ttatgttctt tgaatatcat ggacgatgac tccattgctt ctccttcata ctttgtatgg
      961 tagccattaa aatgtacttc cttagtctgg caaaccaatg aaggcctcga gtacacataa
     1021 ctccaagcat gtagggtgtt cctgacataa ctgaagtgac caggccatag caaccaccat
     1081 ggccattgcc tcccgccacc ttcaccgccc ctccctcgct ctgcagtata catcagctgc
     1141 aagctttcca cagtggcttt ccgccgaact tgcccagtgg gttcctagat tacttctgtg
     1201 tgcgagaata gccaaccatg tagtctgaca agaggtattt acaactactt agcctgttca
     1261 gctggaaaga aatcaaaaga taacactgaa ccctgagatg atgtattaca aatcaaaagt
     1321 cccaatccat aaatagccca cctgatgaac tgctaaatga aaagctgatc ctgattgttg
     1381 tccagtgttt ttacattgtc ctaagaaata tttttaacaa gccttcttgg agagttggga
     1441 ttgtttgttt ttaaatgcat ggaatattat tcttaccttt ctgctgggaa gggagccttc
     1501 cgaggggatt cggatgtaga gtccaagtga gaccacaccc tactttcaca gctttttttc
     1561 taccgtttct gcttcttttt aaatagggtg gattaacagt tacaatttta cattaattta
     1621 agagacttaa tcaaatggag ccagtcatcc gtttccctct ctctaaggga agctcattgt
     1681 cttagatgct caataaatgt ttaattaatg gatttgatag agttaagtat cctaaaagtg
     1741 aacgaacctt ctttacaata ctggcatatt tctgtgattc atatttctgt ctgaagtgtg
     1801 aaataatctt ggcttttttt tttttttttc ctctcttaaa ggtaatcaaa agcctctgtt
     1861 ttagcgaagg gatgccttac attcaggttc agtctaccac tgctttatat gccagtggcc
     1921 tgatagcgtg aaaagaaata tcttctcttt taaactagct ggagacactt ttgtttctaa
     1981 gaacacagcc taaaaattag aaccacttgt ctgtcgccat tttataaaat taatttatgt
     2041 caaacccggg gtgactggct ctagaaggaa aagacataaa acagataacc aactgttttt
     2101 atgaaatgta ttgtccaagg tcaattcaaa aaaattattt aacaaaatta cttattttac
     2161 caaactcatg tatcttgata atgtattttt ctttgattta atgatagtgt ttgtcttcat
     2221 ttcacagaag ttattcatct cttatttttg gtgagacaac tagaacaatt tctgctttat
     2281 gaaacatgca aataaaaatt aggagcaatg ttaaatttaa ctaacttggt ttaatgctag
     2341 tcaaccagta attcttcagt acacttaata tgatgggaaa aacagcatgc tgcattgttg
     2401 caaattttac attttcttta ataaaatgta tttgtttacg gtgtcagtag ggattaaaaa
     2461 tcataattct aaatttaaag tatttaaatt atagaaaaat gattccatta ttttggtaat
     2521 ggaacagaga aatacaaata tagaaacgtt tttatccttt agatcttcta atggtgcaga
     2581 tatttcttaa ccaatttcaa gagtgcctta ttcaaaaaca aaaaacctgc acatagaaaa
     2641 agcaaacatg tttttttaaa aaaagccctg taaaagtggt taatctgtcg tctgattttc
     2701 aatgtatgac tttaatcaat tgcatattcc ttaattctgc aaatgacaat taaaattagg
     2761 aatcagtacc tgaaaacgcc atttatactt ttgagtttat attagaaaca cggttttgat
     2821 gaaatacctt tttcggttca atctttttga attgtgacca caaccaatag ctacacttag
     2881 attctacaga attagtagga aagatacaaa tgagaaggtc tttctagaca atggatctca
     2941 ctgaacatca tcttagtgtg tagacttttt ccactgcagt ggcccacatg tgctatggtg
     3001 acagacggca gttggcatta ccacttatat agtaaaatat ctttaagaca aaaataaaag
     3061 ttatttaaag aatacgctgg ccctgaaaca tgagctgtgc cttaaactac atatcctacc
     3121 tccatgagtg aatgaataag tgggttttcc gcctgcatct atgtgttaag acctattgtt
     3181 tgcttgtatt aattgtagtc ctgggataaa agcacagagg taactttcac tgctgctttt
     3241 tgtactctct ccaatgtttt ggaggaaaaa taagcacaaa caatagccta gagaaactga
     3301 atcgatcata cttgttgcag cttcgacaaa cgtcaatttt gctgcattag aatgggaaac
     3361 atttttcagt ctattgaaat gaattacaaa cgtttttaaa tagaaaatta gttaaaaaat
     3421 tggaggctgc ttaatcgata gctttctcta taaacatact tggatttcac aaataagtaa
     3481 taccgtaaaa atcttcttct ccaaagaaaa atccccacaa ataaatctat tgatacctag
     3541 tgacaagtgg aaccagataa aaatggaatc tataagaatt aacctaattg acagcgctct
     3601 ggagctaatc catttccatt agttatttgt tcacagtagg tactcctaag gacttgttga
     3661 attgcgggct tggcgcccgt tctacgggag agttgcacag ccttcgtgag tggggacaga
     3721 aggcggctcg gcccggtgat tcaggtcgaa attcaagctg aacagcctgc tgagaggtgg
     3781 gatccaccat ccggacagtg gggggctttg ggggtgctgt gagactgggc tgcgacccag
     3841 gtccagcagg gagtgtgcgg cacagaccac aaggtcggcg aggcccccta acccgcgccc
     3901 ggccgggaac ccgcagacca gcgacggggc agctgcgggg ccaggagcgc cccaagacgg
     3961 gcgggcgctg aacccgagcc cctgtcgccg ccctgggccc gaacttccgc cctcccagga
     4021 cctgtcccgg ccgccccgag cggtactcga aggccggggc cgagatgcca ccttccgcag
     4081 gccgcgggaa aggcgcgccg agtcctgcag ctgctctccc ggttcgggaa acgcgcgggg
     4141 cgggggcgtc gggcttggga caggggagga taccagggcc accttcccca acccaggccg
     4201 cgggggcccg gcctccccga tgcagaccac agcgccctca cgggctgccc tcaggccgcg
     4261 cagcgggcag ccgccagccg tcaccccggg gagcgtccgt ggggtgccca ggcaccccac
     4321 cccggcccgg ggcgctcaga cggcagcaga ctgctgggcg gcgcggggac tactttccac
     4381 cgccccctcg cgccccgccc cttgtcctcg cgcggcggaa cgctccgcgc tgcgccggtg
     4441 gcggcaggat acagcggctt ctgcgcgact tataagagct ccttgtgcgg cgccatttta
     4501 agcctctcgg tctgtggcag cagcgttggc ccggccccgg gagcggagag cgaggggagg
     4561 cggagacgga ggaaggtctg aggagcagct tcagtccccg ccgagccgcc accgcaggtc
     4621 gaggacggtc ggactcccgc ggcgggagga gcctgttccc ctgaggtgct tgggcgctcc
     4681 tttccttatc cttccggggc tgctcccgct tcctctcgga gccaaacttc gtagcaggcg
     4741 cgcggtccgg acggcgggct gggcgcagcc gggaggcctg gggttgggag cggggagctc
     4801 aggtggggga cggtgagggt ggcccgcgcc cgggacgcgg agggcggcgg ccgggcccgg
     4861 gttccggtcg cgctgcctct ctggggccct gggggcatcg cttgcgggga gggggcgccg
     4921 cgggggcgcg tacaggagcc cggatggcag gcggggtggg ggtgggggtt gggggtctgt
     4981 ggtttccgtc cggggctctg gccttggccg agtttggggg agggacccgg tgcctcggga
     5041 tgcgccgggc cctgggtggg gggcggggtg gggacggggg gctccgcctt ctcagctctt
     5101 gcggcgagtt ggggttcggg cgctgaggca gagacgccac cctaagtccc atcagtcctg
     5161 gggatcggac cagtggactt tctcttaaga tttcctcttt cattcttaag aatagaagtg
     5221 ttattatttt ttttaatgcc ctggctatgt gagtttgaat cgaagcaact ttaaacctta
     5281 gagcaactaa actctaagtg cagcgggtgc gatgcgtcag tagggtgagc acataaaaaa
     5341 tccatgtctt gcacctgtat tttagcgtac tatgcaggtg agtgaaagca gtggataatg
     5401 tactgggagt cttatggatt tatggtagtg ggtatgagac cctggtgaaa taagggggtg
     5461 gaggaaggcg aaggtgatgg cttactgttt cttaccaagt gaactgcagg attcagcctc
     5521 tgactcagac cgcttcgaga attttgttcg tagaaataat ttaaatttat tcaaatagtt
     5581 tgatggcagc taaaattgaa ttatagagca cgttttcttt tcagcggagt gaatttttcc
     5641 ttcgctccaa agctggccaa atggaattag cttcctgcat gctgctgctg ctgctgctgc
     5701 tgggcctgag gctacagctc tccctgggca tcatcccagt tgaggaggag aacccggact
     5761 tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga
     5821 cagccgccaa gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag
     5881 ctgccaggat cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca
     5941 tggaccgctt cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag
     6001 acagtggagc cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg
     6061 gcttgagtgc agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct
     6121 ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag
     6181 tgcagcacgc ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg
     6241 acgccgacgt gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca
     6301 tctccaacat ggacattgac gtgatcctag gtggaggccg aaagtacatg tttcgcatgg
     6361 gaaccccaga ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga
     6421 agaatctggt gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca
     6481 ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc
     6541 ctggagacat gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga
     6601 tgacagaggc tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg
     6661 agggtggtcg catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga
     6721 cgatcatgtt cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc
     6781 tgagcctcgt cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag
     6841 ggagctccat cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc
     6901 tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg
     6961 agagcgagag cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga
     7021 cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg
     7081 gcgtgcagga gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct
     7141 acaccgcctg cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggttaac
     7201 ccgtggtccc cgcgttgctt cctctgctgg ccgggacatc aggtggcccc cgctgaattg
     7261 gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac
     7321 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     7381 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     7441 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     7501 atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta aaatgtaact
     7561 gtattcagcg atgacgaaat tcttagctat tgtaatactg cgatgagtgg cagggcgggg
     7621 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
     7681 tgataataag cggatgaatg gcagaaattc gccggatctt tgtgaaggaa ccttacttct
     7741 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     7801 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     7861 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     7921 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     7981 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     8041 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     8101 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     8161 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     8221 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     8281 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     8341 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     8401 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     8461 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8521 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgtcg accgatgccc
     8581 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     8641 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     8701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9121 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9181 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9241 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9301 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9361 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9421 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9481 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9541 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9601 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9661 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9721 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9781 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9841 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9901 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9961 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10021 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10081 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10141 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10201 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10261 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10321 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10381 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10441 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10501 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10561 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10621 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10681 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10741 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10801 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10861 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10921 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10981 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    11041 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag
    11101 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcccaa
    11161 gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct ttaaaaaacc
    11221 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    11281 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    11341 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatggta
    11401 ctgtaactga gctaacataa
//
