LOCUS       40924_39093        6256 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPBhBCTNf2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..357
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    358..496
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     CDS             512..2017
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             2551..2625
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88321.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2627..2692
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2822..2842
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    3267..3401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3714..4302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4476..5333)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5334..5438)
                     /label=AmpR promoter
     rep_origin      5465..5920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     regulatory      complement(6088..6184)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      6088..6184
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    6110..6231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg
       61 ggggcccggc ctccccgatg cagaccacag cgccctcacg ggctgccctc aggccgcgca
      121 gcgggcagcc gccagccgtc accccgggga gcgtccgtgg ggtgcccagg caccccaccc
      181 cggcccgggg cgctcagacg gcagcagact gctgggcggc gcggggacta ctttccaccg
      241 ccccctcgcg ccccgcccct tgtcctcgcg cggcggaacg ctccgcgctg cgccggtggc
      301 ggcaggatac agcggcttct gcgcgactta taagagctcc ttgtgcggcg ccattttaag
      361 cctctcggtc tgtggcagca gcgttggccc ggccccggga gcggagagcg aggggaggcg
      421 gagacggagg aaggtctgag gagcagcttc agtccccgcc gagccgccac cgcaggtcga
      481 ggacggtcgg actcccgacc tcgaagcttc ctgcatgctg ctgctgctgc tgctgctggg
      541 cctgaggcta cagctctccc tgggcatcat cccagttgag gaggagaacc cggacttctg
      601 gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc
      661 cgccaagaac ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc
      721 caggatccta aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga
      781 ccgcttccca tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag
      841 tggagccaca gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt
      901 gagtgcagcc gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt
      961 gatgaatcgg gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca
     1021 gcacgcctcg ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc
     1081 cgacgtgcct gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc
     1141 caacatggac attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac
     1201 cccagaccct gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa
     1261 tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga
     1321 gctcatgcag gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg
     1381 agacatgaaa tacgagatcc accgagactc cacactggac ccctccctga tggagatgac
     1441 agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg
     1501 tggtcgcatc gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat
     1561 catgttcgac gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag
     1621 cctcgtcact gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag
     1681 ctccatcttc gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct
     1741 atacggaaac ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag
     1801 cgagagcggg agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca
     1861 cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt
     1921 gcaggagcag accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac
     1981 cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gttaacccgt
     2041 ggtccccgcg ttgcttcctc tgctggccgg gacatcaggt ggcccccgct gaattggaat
     2101 cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg
     2161 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     2221 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     2281 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     2341 gagagatcct cataaaggcc aagaagggcg gaaagtccaa attgtaaaat gtaactgtat
     2401 tcagcgatga cgaaattctt agctattgta atactgcgat gagtggcagg gcggggcgta
     2461 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat
     2521 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg
     2581 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     2641 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     2701 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     2761 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     2821 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     2881 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     2941 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     3001 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     3061 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     3121 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     3181 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3241 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3361 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgtcgaccg atgcccttga
     3421 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     3481 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5461 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5881 tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg
     5941 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     6001 ccatgataag taagtaatat taaggtacgt ggaggtttta cttgctttaa aaaacctccc
     6061 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atggtactgt
     6241 aactgagcta acataa
//
