LOCUS       40924_39088        5953 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSEAPBhBCTNf1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5953)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5953)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      60..162
                     /label=human BCTN promoter region
                     /note="human BCTN promoter region"
                     /regulatory_class="promoter"
     misc_feature    163..201
                     /label=5' UTR of human BCTN
                     /note="5' UTR of human BCTN"
     CDS             209..1714
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     CDS             2248..2322
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88327.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     intron          2324..2389
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2519..2539
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2964..3098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3411..3999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4173..5030)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5031..5135)
                     /label=AmpR promoter
     rep_origin      5162..5617
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     regulatory      complement(5785..5881)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      5785..5881
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    5807..5928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg
       61 ccccttgtcc tcgcgcggcg gaacgctccg cgctgcgccg gtggcggcag gatacagcgg
      121 cttctgcgcg acttataaga gctccttgtg cggcgccatt ttaagcctct cggtctgtgg
      181 cagcagcgtt ggcccggccc cagcttcctg catgctgctg ctgctgctgc tgctgggcct
      241 gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa
      301 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
      361 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
      421 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
      481 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
      541 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
      601 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
      661 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
      721 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
      781 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
      841 catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc
      901 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
      961 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct
     1021 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga
     1081 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     1141 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     1201 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     1261 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     1321 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     1381 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     1441 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     1501 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     1561 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     1621 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     1681 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt
     1741 ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga
     1801 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
     1861 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     1921 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     1981 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     2041 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca
     2101 gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt
     2161 tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat
     2221 aagcggatga atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt
     2281 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     2341 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     2401 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     2461 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     2521 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     2581 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     2641 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     2701 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     2761 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     2821 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     2881 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2941 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttatcatg tctggatccg tcgaccgatg cccttgagag
     3121 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3181 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     3241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3841 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5161 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5221 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5281 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5341 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5401 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5461 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5521 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5581 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     5641 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     5701 tgataagtaa gtaatattaa ggtacgtgga ggttttactt gctttaaaaa acctcccaca
     5761 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5821 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5881 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatg gtactgtaac
     5941 tgagctaaca taa
//
