LOCUS       pSEAP2-Basic.        4677 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless mammalian vector encoding secreted alkaline 
            phosphatase, for cloning promoter and enhancer elements.
ACCESSION   .
VERSION     .
KEYWORDS    pSEAP2-Basic
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..72
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             74..1582
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
     polyA_signal    complement(1640..1761)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2180..2768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2942..3799)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3800..3904)
                     /label=AmpR promoter
     rep_origin      3931..4386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4517..4565
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4579..4670
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttcg
       61 aatcgcgaat tcgcccacca tgctgctgct gctgctgctg ctgggcctga ggctacagct
      121 ctccctgggc atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc
      181 cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat
      241 catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg
      301 gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt
      361 ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac
      421 ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg
      481 ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa
      541 gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc
      601 cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc
      661 ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga
      721 cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta
      781 cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg
      841 gctggcgaag cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc
      901 cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga
      961 gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg
     1021 cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca
     1081 tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc
     1141 cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga
     1201 ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct
     1261 ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc
     1321 aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc
     1381 cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt
     1441 ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt
     1501 catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc
     1561 gccccccgcc ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg
     1621 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1681 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1741 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1801 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     1861 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1921 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1981 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2581 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3901 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     3961 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4021 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4081 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4141 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4201 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4261 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4321 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4381 caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4441 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg
     4501 tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4561 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4621 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata
//
