LOCUS       V003393                 5885 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003393
VERSION     V003393
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..1122)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1123..1200)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    complement(1201..1231)
                     /label="5FR"
                     /note="5FR"
     misc_feature    1386..1526
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1712..2300)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2474..3331)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3332..3423)
                     /label="AmpR promoter"
     terminator      complement(3443..3470)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(3562..3648)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    complement(3649..3768)
                     /label="5SRNA"
                     /note="5SRNA"
     CDS             complement(4096..5553)
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     misc_feature    complement(5563..5619)
                     /label="srPDI"
                     /note="srPDI"
     protein_bind    complement(5640..5656)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5664..5693)
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
ORIGIN
        1 cagatcaatt cgcgctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
       61 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      121 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
      181 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
      241 gcgaaaatcc tgtttgatgg tggttgacgg cgggatataa catgagctgt cttcggtatc
      301 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
      361 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
      421 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
      481 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
      541 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
      601 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
      661 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
      721 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
      781 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
      841 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
      901 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
      961 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     1021 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     1081 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     1141 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc
     1201 aacgtaaatg catgccgctt cgccttcgcg cgcgaattga tctgctgcct cgcgcgtttc
     1261 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1321 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1381 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     1441 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     1501 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     1561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     2161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2761 gtttgcgcaa cgttgttgcc attgctgtag gcatcgtggt gtcacgctcg tcgtttggta
     2821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3061 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     3121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3421 aaaaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg
     3481 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac
     3541 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3601 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt
     3661 tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt ttcacttctg
     3721 agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa ttctgtttta
     3781 tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc tctcatccgc
     3841 caaaacagcc aagcttaagg tgcacggccc acgtggccac tagtacttct cgagctctgt
     3901 acatgtccgc ggtcgcgacg tacgcgtatc gatggcgcca gctgcaggcg gccgccatat
     3961 gcatcctagg cctattaata ttccggagta tacgtagccg gctaacgtta acaaccggta
     4021 cctctagaac tatagctagc atgcgcaaat ttaaagcgct gatatcgatc gcgcgcagat
     4081 ctggcttctg cactacagtt catccttcac ggctttctga tcgtcgtctt cttccatatc
     4141 tggctctaaa gcttcttcta ggtcgaggtc gtcattgtcc cccgctccat cctggccacc
     4201 gctctccaag aatttcttaa aaccatctag tgtccgctca ccgttgtaat caatgaccgt
     4261 tctgtctgca cttgctggga agaacttgag tgtgggaaag ctgtgcacct tcacagcttc
     4321 cacctcattg gctgttgagt ccatcttagc gatgacgata ttctcatggt ctttgtatgt
     4381 ctctcccagt ttatcccaaa tcggggctag ctgcttgcag tgaccacacc agggagcata
     4441 gaattcaaca aacacgttct ttttctcatc aaaagcaacc tcctcaaagt ttttcccaac
     4501 tagcactttc actggctgct tgtcccagtc ttcaggcagt tcctggctca tcaggtgggg
     4561 cttgatcttg ccctccagga agtggtggca aaattgtgtg atcttctcag ctgtcagctc
     4621 gtctgactcc ggtttgtact tggtgagctc ttcctccagg gtaataagcc gcacagctgg
     4681 acattcctcc ttcttcaggc caaagaactc aagtatgcgc tggttgtcag tgtggtcact
     4741 atcgatgaag atgaacagga tcttgccctt aaagccctcg gccgctttct taaagttgct
     4801 caatttgcca tcgtagtcag acacactctt gggcaggaac agcagaatat gtgtcttgat
     4861 ttcacctccg aaaatctttg gagctgtctg ttcagtgaac tcgatgacca aaggcagctg
     4921 gttgtgcttg atgaagtcta atagcttctc cttggtgatc tcaccttcaa aattgttgcg
     4981 gccttcatca aacttcttaa agaggaccac cccatccttg tccagctggt acttggaaaa
     5041 cacatcgcta ttggaagtga ttccaaaagg tatgtcatca acagcctctg ctgccagcaa
     5101 gaactgcttg gcggagtctg accctgcgtc cttgaagaag ccgatgaccg tcacttcgct
     5161 tgagtccacc aaggactctg cagctgcagt gtcagacagg gttgtggctg ctgggcctgt
     5221 gcgtttcttc agccagttca caatgtcgtc agcttccctg ccagctgtat attcctttgg
     5281 ggaggctgtg tctccattct tgaagaactt gattgtgggg tagccacgga caccatactg
     5341 ctgggccagg tcagactctt ctgtggcgtc cacctttgct agtcggatct cagagccttc
     5401 tgccttcagt tttgcagcag ctttggcata ctctggggcc agtgctttgc agtggccaca
     5461 ccatggggca tagaactcca ccagcaggta gttgtgcgcc gccggctctg cgaagttgct
     5521 cttcttcagc accaggacgt tgtcctcctc ctccagagcg tcggcgccca ccctagccgc
     5581 ccaggccagg gccaggcaca gcaaagcacg gctcagcatg gtctgtttcc tgtgtgaaat
     5641 tgttatccgc tcacaattcc acacattata cgagccggat gattaattgt caacagctca
     5701 tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg
     5761 agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag
     5821 cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat gataagctgt
     5881 caaac
//
