LOCUS       40924_39063        4889 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSDU1deltamob, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Wang R, Lin C, Lin J, Pang X, Liu X, Zhang C, Lin J, Chen L.
  TITLE     Construction of novel pJRD215-derived plasmids using chloramphenicol
            acetyltransferase (cat) gene as a selection marker for 
            Acidithiobacillus caldus
  JOURNAL   PLoS ONE 12 (8), e0183307 (2017)
  PUBMED    28813510
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Wang R, Chen L, Lin J, Lin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2017) State Key Laboratory of Microbial 
            Technology, School of Life Sciences, Shandong University, 27 Shanda 
            South Road, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 4889)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2017"; volume: "12"; issue: "8"; pages: "e0183307"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2017) State Key Laboratory of Microbial Technology, School 
            of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, 
            Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit v. 7.1.3.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(131..525)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             658..1626
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             2140..2976
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             2966..3814
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     misc_feature    complement(4011..4067)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             4156..4812
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgcactcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
       61 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta
      121 ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc
      181 cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg
      241 tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc
      301 ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct
      361 attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa
      421 agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt
      481 agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct
      541 cggtcgatca ttcagctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg
      601 taggctatca tggaggcaca gcattgagcc gaaaagcaaa agcaacagcg aggcagcatg
      661 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
      721 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
      781 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
      841 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
      901 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
      961 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     1021 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     1081 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     1141 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     1201 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     1261 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     1321 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     1381 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     1441 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     1501 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     1561 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     1621 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     1681 gggggtttta tggaatacga aaaaagcgct tcagggtcgg tctacctgat caaaagtgac
     1741 aagggctatt ggttgcccgg tggctttggt tatacgtcaa acaaggccga ggctggccgc
     1801 ttttcagtcg ctgatatggc cagccttaac cttgacggct gcaccttgtc cttgttccgc
     1861 gaagacaagc ctttcggccc cggcaagttt ctcggtgact gatatgaaag accaaaagga
     1921 caagcagacc ggcgacctgc tggccagccc tgacgctgta cgccaagcgc gatatgccga
     1981 gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg ctgaccgacg acgaatacga
     2041 ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag ggcgggggta gtgaccccgc
     2101 cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa tggctaccca taagcctatc
     2161 aatattctgg aggcgttcgc agcagcgccg ccaccgctgg actacgtttt gcccaacatg
     2221 gtggccggta cggtcggggc gctggtgtcg cccggtggtg ccggtaaatc catgctggcc
     2281 ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc tggaggtggg cgaactgccc
     2341 accggcccgg tgatctacct gcccgccgaa gacccgccca ccgccattca tcaccgcctg
     2401 cacgcccttg gggcgcacct cagcgccgag gaacggcaag ccgtggctga cggcctgctg
     2461 atccagccgc tgatcggcag cctgcccaac atcatggccc cggagtggtt cgacggcctc
     2521 aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca cgctgcgccg gttccacatc
     2581 gaggaagaaa acgccagcgg ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc
     2641 gccgataccg ggtgctctat cgtgttcctg caccatgcca gcaagggcgc ggccatgatg
     2701 ggcgcaggcg accagcagca ggccagccgg ggcagctcgg tactggtcga taacatccgc
     2761 tggcagtcct acctgtcgag catgaccagc gccgaggccg aggaatgggg tgtggacgac
     2821 gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc
     2881 gctgatcggt ggttcaggcg gcatgacggc ggggtgctca agcccgccgt gctggagagg
     2941 cagcgcaaga gcaagggggt gccccgtggt gaagcctaag aacaagcaca gcctcagcca
     3001 cgtccggcac gacccggcgc actgtctggc ccccggcctg ttccgtgccc tcaagcgggg
     3061 cgagcgcaag cgcagcaagc tggacgtgac gtatgactac ggcgacggca agcggatcga
     3121 gttcagcggc ccggagccgc tgggcgctga tgatctgcgc atcctgcaag ggctggtggc
     3181 catggctggg cctaatggcc tagtgcttgg cccggaaccc aagaccgaag gcggacggca
     3241 gctccggctg ttcctggaac ccaagtggga ggccgtcacc gctgaatgcc atgtggtcaa
     3301 aggtagctat cgggcgctgg caaaggaaat cggggcagag gtcgatagtg gtggggcgct
     3361 caagcacata caggactgca tcgagcgcct ttggaaggta tccatcatcg cccagaatgg
     3421 ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg
     3481 cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt
     3541 gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca
     3601 gcggctgtgt ggctggatcg accccggcaa aaccggcaag gcttccatag ataccttgtg
     3661 cggctatgtc tggccgtcag aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt
     3721 gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg
     3781 caagtacgac atcacccggc ccaaggcggc aggctgaccc cccccactct attgtaaaca
     3841 agacattttt atcttttata ttcaatggct tattttcctg ctaattggta ataccatgaa
     3901 aaataccatg ctcagaaaag gcttaacaat attttgaaaa attgcctact gagcgctgcc
     3961 gcacagctcc ataggccgct ttcctggctt tgcttccaga tgtatgctct aagcttgcat
     4021 gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcatg cattcatgtt
     4081 tgacagctta tcatccataa ggtttaatgc ggtagtttat cacagttaaa ttgctaacgc
     4141 agtcaggcac cgtgtatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     4201 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     4261 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     4321 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     4381 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     4441 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     4501 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     4561 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     4621 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     4681 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtc
     4741 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     4801 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg
     4861 cctgaataag tgataataag cggatgaat
//
