LOCUS       40924_39058        6424 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSDU1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Wang R, Lin C, Lin J, Pang X, Liu X, Zhang C, Lin J, Chen L.
  TITLE     Construction of novel pJRD215-derived plasmids using chloramphenicol
            acetyltransferase (cat) gene as a selection marker for 
            Acidithiobacillus caldus
  JOURNAL   PLoS ONE 12 (8), e0183307 (2017)
  PUBMED    28813510
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Wang R, Chen L, Lin J, Lin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2017) State Key Laboratory of Microbial 
            Technology, School of Life Sciences, Shandong University, 27 Shanda 
            South Road, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 6424)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6424)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2017"; volume: "12"; issue: "8"; pages: "e0183307"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2017) State Key Laboratory of Microbial Technology, School 
            of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, 
            Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit v. 7.1.3.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(131..525)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(552..836)
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC"
                     /label=mobC
                     /protein_id="AWD72702.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(552..836)
                     /gene="mobC"
                     /label=mobC
     oriT            867..954
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             1783..2196
                     /codon_start=1
                     /gene="mobB"
                     /product="MobB"
                     /label=mobB
                     /protein_id="AWD72704.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            1783..2196
                     /gene="mobB"
                     /label=mobB
     CDS             2193..3161
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3675..4511
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4501..5349
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     misc_feature    complement(5546..5602)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             5691..6347
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgcactcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
       61 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta
      121 ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc
      181 cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg
      241 tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc
      301 ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct
      361 attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa
      421 agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt
      481 agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct
      541 cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg
      601 tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg
      661 ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc
      721 ttgcgctctt gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct
      781 cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg
      841 acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc
      901 cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa ctggcctaac
      961 ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa aagcaaaagc
     1021 aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc aggtcgggcg
     1081 gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat gcccgcgaca
     1141 tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag cggcccgccg
     1201 actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg
     1261 aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg tccgagttcg
     1321 cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat gccggtggcg
     1381 gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc atcgagcggc
     1441 ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc ggggcacaga
     1501 agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca tgggccgacc
     1561 atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac agaacacttg
     1621 aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg
     1681 aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac accgccaacg
     1741 cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc aatcgacaga
     1801 gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct ggcccagagc
     1861 atggcgacac tggccgacga agcccggcag gtcatgagcc agacccagca ggccagcgag
     1921 gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg ggtggagctg
     1981 gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag cgcccggagc
     2041 gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc catgatgcct
     2101 acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact gacaaccgag
     2161 gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact ttgcaggcca
     2221 taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc agggacgcca
     2281 ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag aacacgccat
     2341 ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc gagcaggagc
     2401 ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac atgaaagccg
     2461 agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag gcatgggtca
     2521 aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg ctggccagcg
     2581 agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg gcgggcttca
     2641 ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg ctgctgcgtg
     2701 aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct ggccagcaga
     2761 tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc gaactgcccg
     2821 agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc gagatggccg
     2881 ggctggtcaa gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc
     2941 tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag gccagcccag
     3001 cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc gtcagcaagg
     3061 tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca ccggcacccc
     3121 gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg cgttggtact
     3181 cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa tacgaaaaaa
     3241 gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg cccggtggct
     3301 ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat atggccagcc
     3361 ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc ggccccggca
     3421 agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga cctgctggcc
     3481 agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa agggatgcgt
     3541 cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa
     3601 ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc aactgcctgc
     3661 aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg ttcgcagcag
     3721 cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc ggggcgctgg
     3781 tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca cagattgcag
     3841 gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg
     3901 ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg cacctcagcg
     3961 ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc
     4021 ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc
     4081 tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca
     4141 tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt
     4201 tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca
     4261 gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga
     4321 ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct
     4381 tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg
     4441 acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc
     4501 gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt
     4561 ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac
     4621 gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc
     4681 gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg
     4741 cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag
     4801 tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc gctggcaaag
     4861 gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag
     4921 cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg
     4981 ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg
     5041 atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg
     5101 gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc
     5161 ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc
     5221 agtggttcga ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc
     5281 gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag
     5341 gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa
     5401 tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag aaaaggctta
     5461 acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg ccgctttcct
     5521 ggctttgctt ccagatgtat gctctaagct tgcatgcctg caggtcgact ctagaggatc
     5581 cccgggtacc gagctcgaat tcatgcattc atgtttgaca gcttatcatc cataaggttt
     5641 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atggagaaaa
     5701 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     5761 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     5821 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     5881 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     5941 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     6001 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     6061 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     6121 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     6181 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     6241 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
     6301 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     6361 gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat
     6421 gaat
//
