LOCUS       pSD1       10904 bp DNA     circular SYN 02-OCT-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pSD1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   111111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10904)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(408..483)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      complement(508..692)
                     /note="sgRNA; constitutive expression"
                     /regulatory_class="promoter"
     terminator      complement(705..732)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(748..819)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(835..4941)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="dCas9"
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10 mutation in the 
                     RuvC-like domain and the H840A mutation in the HNH domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(4993..5011)
                     /label=tet operator
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             5121..5744
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=tetR from transposon Tn10
                     /note="TetR"
                     /note="TetR binds to the tetracycline operator tetO to
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(5985..5999)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase recognition and cleavage site
                     /note="enterokinase site"
                     /translation="DDDDK"
     CDS             7490..8137
                     /gene="cat"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     complement(8683..8699)
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8707..8723
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        8731..8761
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8776..8797
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9085..9673)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9844..10704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10705..10809)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaaaagca ccgactcggt
      421 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      481 aacagaagag cctgacatgg ctcttcatag tcattgtagc atgtttgttg tgattttttt
      541 cacataaatt atgtacaatt ttcatcaaat gtatgtcaaa acatagaaac tgtactaatt
      601 tgtgtaacag taatatgcta attgcattct gttacacaac agttataatg ttcactattt
      661 tcattgcttt aaaagttaat ttaggagtat atcccgggta taaacgcaga aaggcccacc
      721 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
      781 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggctc tagttcagtc
      841 acctcctagc tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg attgatggat
      901 aagagtggca tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa ttgttgtatc
      961 aaaatattta aaagcagcgg gagctccaag attcgtcaac gtaaataaat gaataatatt
     1021 ttctgcttgt tcacgtattg gtttgtctct atgtttgtta tatgcactaa gaactttatc
     1081 taaattggca tctgctaaaa taacacgctt agaaaattca ctgatttgct caataatctc
     1141 atctaaataa tgcttatgct gctccacaaa caattgtttt tgttcgttat cttctggact
     1201 acccttcaac ttttcataat gactagctaa atataaaaaa ttcacatatt tgcttggcag
     1261 agccagctca tttccttttt gtaattctcc ggcactagcc agcatccgtt tacgaccgtt
     1321 ttctaactca aaaagactat atttaggtag tttaatgatt aagtcttttt taacttcctt
     1381 atatccttta gcttctaaaa agtcaatcgg atttttttca aaggaacttc tttccataat
     1441 tgtgatccct agtaactctt taacggattt taacttcttc gatttccctt tttccacctt
     1501 agcaaccact aggactgaat aagctaccgt tggactatca aaaccaccat atttttttgg
     1561 atcccagtct tttttacgag caataagctt gtccgaattt ctttttggta aaattgactc
     1621 cttggagaat ccgcctgtct gtacttctgt tttcttgaca atattgactt ggggcatgga
     1681 caatactttg cgcactgtgg caaaatctcg ccctttatcc cagacaattt ctccagtttc
     1741 cccattagtt tcgattagag ggcgtttgcg aatctctcca tttgcaagtg taatttctgt
     1801 tttgaagaag ttcatgatat tagagtaaaa gaaatatttt gcggttgctt tgcctatttc
     1861 ttgctcagac ttagcaatca ttttacgaac atcataaact ttataatcac catagacaaa
     1921 ctccgattca agttttggat atttcttaat caaagcagtt ccaacgacgg catttagata
     1981 cgcatcatgg gcatgatggt aattgttaat ctcacgtact ttatagaatt ggaaatcttt
     2041 tcggaagtca gaaactaatt tagattttaa ggtaatcact ttaacctctc gaataagttt
     2101 atcattttca tcgtatttag tattcatgcg actatccaaa atttgtgcca catgcttagt
     2161 gatttggcga gtttcaacca attggcgttt gataaaacca gctttatcaa gttcactcaa
     2221 acctccacgt tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt
     2281 tagaagttgt ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc
     2341 cgatttacca cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt
     2401 aaggaaactt tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa
     2461 ttcttggtcc acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt
     2521 ttgcaattga gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat
     2581 accttcttcg attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg
     2641 attttcacgt gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac
     2701 caattcatca acaactttta cagtctgtaa aatacctttt ttaatagcag ggctaccagc
     2761 taaatttgca atatgttcat gtaaactatc gccttgtcca gacacttgtg ctttttgaat
     2821 gtcttcttta aatgtcaaac tatcatcatg gatcagctgc ataaaattgc gattggcaaa
     2881 accatctgat ttcaaaaaat ctaatattgt tttgccagat tgcttatccc taataccatt
     2941 aatcaatttt cgagacaaac gtccccaacc agtataacgg cgacgtttaa gctgtttcat
     3001 caccttatca tcaaagaggt gagcatatgt tttaagtctt tcctcaatca tctccctatc
     3061 ttcaaataag gtcaatgtta aaacaatatc ctctaagata tcttcatttt cttcattatc
     3121 caaaaaatct ttatctttaa taatttttag caaatcatgg taggtaccta atgaagcatt
     3181 aaatctatct tcaactcctg aaatttcaac actatcaaaa cattctattt ttttgaaata
     3241 atcttctttt aattgcttaa cggttacttt tcgatttgtt ttgaagagta aatcaacaat
     3301 ggctttcttc tgttcacctg aaagaaatgc tggttttcgc attccttcag taacatattt
     3361 gacctttgtc aattcgttat aaaccgtaaa atactcataa agcaaactat gttttggtag
     3421 tactttttca tttggaagat ttttatcaaa gtttgtcatg cgttcaataa atgattgagc
     3481 tgaagcacct ttatcgacaa cttcttcaaa attccatggg gtaattgttt cttcagactt
     3541 ccgagtcatc catgcaaaac gactattgcc acgcgccaat ggaccaacat aataaggaat
     3601 tcgaaaagtc aagatttttt caatcttctc acgattgtct tttaaaaatg gataaaagtc
     3661 ttcttgtctt ctcaaaatag catgcagctc acccaagtga atttgatggg gaatagagcc
     3721 gttgtcaaag gtccgttgct tgcgcagcaa atcttcacga tttagtttca ccaataattc
     3781 ctcagtacca tccatttttt ctaaaattgg tttgataaat ttataaaatt cttcttggct
     3841 agctccccca tcaatataac ctgcatatcc gttttttgat tgatcaaaaa agatttcttt
     3901 atacttttct ggaagttgtt gtcgaactaa agcttttaaa agagtcaagt cttgatgatg
     3961 ttcatcgtag cgtttaatca ttgaagctga taggggagcc ttagttattt cagtatttac
     4021 tcttaggata tctgaaagta aaatagcatc tgataaattc ttagctgcca aaaacaaatc
     4081 agcatattga tctccaattt gcgccaataa attatctaaa tcatcatcgt aagtatcttt
     4141 tgaaagctgt aatttagcat cttctgccaa atcaaaattt gatttaaaat taggggtcaa
     4201 acccaatgac aaagcaatga gattcccaaa taagccattt ttcttctcac cggggagctg
     4261 agcaatgaga ttttctaatc gtcttgattt actcaatcgt gcagaaagaa tcgctttagc
     4321 atctactcca cttgcgttaa tagggttttc ttcaaataat tgattgtagg tttgtaccaa
     4381 ctggataaat agtttgtcca catcactatt atcaggattt aaatctccct caatcaaaaa
     4441 atgaccacga aacttaatca tatgcgctaa ggccaaatag attaagcgca aatccgcttt
     4501 atcagtagaa tctaccaatt tttttcgcag atgatagata gttggatatt tctcatgata
     4561 agcaacttca tctactatat ttccaaaaat aggatgacgt tcatgcttct tgtcttcttc
     4621 caccaaaaaa gactcttcaa gtcgatgaaa gaaactatca tctactttcg ccatctcatt
     4681 tgaaaaaatc tcctgtagat aacaaatacg attcttccga cgtgtatacc ttctacgagc
     4741 tgtccgtttg agacgagtcg cttccgctgt ctctccactg tcaaataaaa gagcccctat
     4801 aagatttttt ttgatactgt ggcggtctgt atttcccaga accttgaact ttttagacgg
     4861 aaccttatat tcatcagtga tcaccgccca tccgacgcta tttgtgccga tagctaagcc
     4921 tattgagtat ttcttatcca tgaaacggcc tcccagatct gttaacggta ccatcaagct
     4981 tattttaatt atactctatc aatgatagag tgtcaatatt ttttttagtt tttcatgaac
     5041 tcgaggggat ccaaataaaa aactagtttg acaaataact ctatcaatga tataatgtca
     5101 acaaaaagga ggaattaatg atgtctagat tagataaaag taaagtgatt aacagcgcat
     5161 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     5221 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     5281 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     5341 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     5401 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     5461 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     5521 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     5581 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     5641 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     5701 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc ttaactgcag gcatgcaagc
     5761 ttttaaaaag caaatatgag ccaaataaat atattctaat tctacaaaca aaaatttgag
     5821 caaattcagt gtcgattttt taagacactg cccagttaca tgcaaattaa aattttcatg
     5881 attttttata gttcctaaca gggttaaaat ttgtataacg aaagtataat gtttatataa
     5941 cgttagtata ataaagcatt ttaacattat acttttgata atcgtttatc gtcgtcatca
     6001 caataacttt taaaatactc gtgcataatt caacagctga cctcccaata actacatggt
     6061 gttatcggga ggtcagctgt tagcacttat attttgttat tgttcttcct cgatttcgtc
     6121 tatcattttg tgattaattt ctcttttttc ttgttctgtt aagtcataaa gttcactagc
     6181 taaatactct ttttgtttcc aaatataaaa aatttgatag atatattcgg ttggatcaat
     6241 ttcttttaag taatctaaat ccccattttt taatttcttt ttagcctctt taaataatcc
     6301 tgaataaact aatacctgtt tacctttaag tgatttataa aatgcatcaa agactttttg
     6361 atttattaaa taatcactat ctttaccaga atacttagcc atttcatata attctttatt
     6421 attattttgt cttatttttt gaacttgaac ttgtgttatt tctgaaatgc ccgttacatc
     6481 acgccataaa tctaaccatt cttgttggct aatataatat cttttatctg tgaaatacga
     6541 tttatttact gcaattaaca catgaaaatg aggattataa tcatctcttt ttttattata
     6601 tgtaatctct aacttacgaa catatccctt tataacacta cctacttttt ttctctttat
     6661 aagttttcta aaagaattat tataacgttt tatttcattt tctaattcat cactcattac
     6721 attaggtgta gtcaaagtta aaaagataaa ctcctttttc tcttgctgct taatatattg
     6781 catcatcaaa gataaaccca atgcatcttt tctagctttt ctccaagcac agacaggaca
     6841 aaatcgattt ttacaagaat tagctttata taatttctgt ttttctaaag ttttatcagc
     6901 tacaaaagac agaaatgtat tgcaatcttc aactaaatcc atttgattct ctccaatatg
     6961 acgtttaata aatttctgaa atacttgatt tctttgtttt ttctcagtat acttttccat
     7021 gttataacac ataaaaacaa cttagttttc acaaactatg acaataaaaa aagttgcttt
     7081 ttcccctttc tatgtatgtt ttttactatt catttaaaac gatacattaa taggtacgaa
     7141 aaagcaactt tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc
     7201 gccggcaata gttaccctta ttatcaagat aagaaagaaa aggatttttc gctacgctca
     7261 aatcctttaa aaaaacacaa aagaccacat tttttaatgt ggtcttttat tcttcaacta
     7321 aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt aaaatatata
     7381 tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag aaagcagaca
     7441 agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa
     7501 taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca
     7561 aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca
     7621 agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa
     7681 tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga
     7741 gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt
     7801 aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg
     7861 ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat
     7921 tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct
     7981 acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc
     8041 tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat
     8101 tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt
     8161 actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa gaaggttttt
     8221 atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct ttttcgcttc
     8281 tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt
     8341 cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt tagttaaggg
     8401 ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt tttattttta
     8461 ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat aatcgataac
     8521 cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa atatttaaat
     8581 tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg taaatcatat
     8641 agttttatgc ctaaaaacct acagaagctt ggcgtaatca tggtcatagc tgtttcctgt
     8701 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8761 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgtccgc
     8821 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8881 aggcggtttg cgtattgggc gctctaccgc ttcctcgctc actgactcgc tgcgctcggt
     8941 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9001 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10861 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
