LOCUS       V003401                13173 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003401
VERSION     V003401
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      341..1683
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(1837..2379)
                     /gene="RAF1"
                     /label="Trans-acting factor D"
                     /note="Trans-acting factor D from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: Q06891"
     CDS             2642..3760
                     /gene="REP1"
                     /label="Partitioning protein REP1"
                     /note="Partitioning protein REP1 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P03871"
     protein_bind    complement(3927..3974)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4308..5195)
                     /gene="REP2"
                     /label="Partitioning protein REP2"
                     /note="Partitioning protein REP2 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P03872"
     regulatory      6345..7326
                     /label="CYC1"
                     /note="CYC1"
                     /regulatory_class="promoter"
     CDS             7350..7910
                     /label="DHFR"
                     /note="mouse dihydrofolate reductase"
     terminator      8022..8269
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        8276..8496
                     /label="URA3 promoter"
     CDS             8497..9297
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(9462..10050)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10224..11081)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11082..11186)
                     /label="AmpR promoter"
     misc_feature    complement(11312..11480)
                     /label="3' UTR of mIghg2b"
                     /note="3' UTR of mIghg2b"
     CDS             complement(11484..11801)
                     /label="mIg-kappa-CL"
                     /note="Mouse immunoglobulin kappa light chain constant
                     region"
     CDS             complement(12126..12380)
                     /label="alpha-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     promoter        complement(12442..12883)
                     /label="GAL1 promoter"
                     /note="inducible promoter, regulated by Gal4"
     terminator      12916..13163
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ttctgaacca gtcctaaaac gagtaaatag gaccggcaat tcttcaagca ataaacagga
       61 ataccaatta ttaaaagata acttagtcag atcgtacaat aaagctttga agaaaaatgc
      121 gccttattca atctttgcta taaaaaatgg cccaaaatct cacattggaa gacatttgat
      181 gacctcattt ctttcaatga agggcctaac ggagttgact aatgttgtgg gaaattggag
      241 cgataagcgt gcttctgccg tggccaggac aacgtatact catcagataa cagcaatacc
      301 tgatcactac ttcgcactag tttctcggta ctatgcatat gatccaatat caaaggaaat
      361 gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag aacagctaaa
      421 gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat cacaggaggt
      481 actagactac ctttcatcct acataaatag acgcatataa gtacgcattt aagcataaac
      541 acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc
      601 gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt
      661 cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
      721 agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca
      781 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa
      841 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
      901 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
      961 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
     1021 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
     1081 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
     1141 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
     1201 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
     1261 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
     1321 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
     1381 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
     1441 atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
     1501 aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg
     1561 gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     1621 ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc
     1681 gttaacaaag atatgctatt gaagtgcaag atggaaacgc agaaaatgaa ccggggatgc
     1741 gacgtgcaag attacctatg caatagatgc aatagtttct ccaggaaccg aaatacatac
     1801 attgtcttcc gtaaagcgct agactatata ttattataca ggttcaaata tactatctgt
     1861 ttcagggaaa actcccaggt tcggatgttc aaaattcaat gatgggtaac aagtacgatc
     1921 gtaaatctgt aaaacagttt gtcggatatt aggctgtatc tcctcaaagc gtattcgaat
     1981 atcattgaga agctgcagcg tcacatcgga taataatgat ggcagccatt gtagaagtgc
     2041 cttttgcatt tctagtctct ttctcggtct agctagtttt actacatcgc gaagatagaa
     2101 tcttagatca cactgccttt gctgagctgg atcaatagag taacaaaaga gtggtaaggc
     2161 ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac agtagacgga gtatactagt
     2221 atagtctata gtccgtggaa ttctaagtgc cagctttata atgtcattct ccttactaca
     2281 gacccgcctg aaagtagaca catcatcatc agtaagcttt gacaaaaagc attgagtagc
     2341 taactcttct atgcaatcta tagctgtttt ataaggcatt caatggacag attgaggttt
     2401 ttgaaacata ctagtgaaat tagccttaat cccttctcga agttaatcat gcattatggt
     2461 gtaaaaaatg caactcgcgt tgctctactt tttcccgaat ttccaaatac gcagctgggg
     2521 tgattgctcg atttcgtaac gaaagttttg tttataaaaa ccgcgaaaac cttctgtaac
     2581 agatagattt ttacagcgct gatatacaat gacatcagct gtaatggaaa ataactgaaa
     2641 tatgaatggc gagagactgc ttgcttgtat taagcaatgt attatgcagc acttccaacc
     2701 tatggtgtac gatgaaagta ggtgtgtaat cgagacgaca agggggactt ttccagttcc
     2761 tgacaattat aagaaataca aaacgttagc atttgcattt gttggacatg tactgaatac
     2821 agacgacaca ccggtaattg aaaaagaact ggattggcct gatcctgcac tagtgtacaa
     2881 tacaattgtc gatcgaatca taaatcaccc agaattatca cagtttatat cggttgcatt
     2941 tattagtcag ttaaaggcca ccatcggaga gggtttagat attaatgtaa aaggcacgct
     3001 aaaccgcagg ggaaagggta tcagaaggcc taaaggcgta ttttttagat acatggaatc
     3061 tccatttgtc aatacaaagg tcactgcatt cttctcttat cttcgagatt ataataaaat
     3121 tgcctcagaa tatcacaata atactaaatt cattctcacg ttttcatgtc aagcatattg
     3181 ggcatctggc ccaaacttct ccgccttgaa gaatgttatt aggtgctcca taattcatga
     3241 atacatttct aagtttgtgg aaagagaaca ggataaaggt catataggag atcaggagct
     3301 accgcctgaa gaggaccctt ctcgtgaact aaacaatgta caacatgaag tcaatagttt
     3361 aacggaacaa gatgcggagg cggatgaagg attgtggggt gaaatagatt cattatgtga
     3421 aaaatggcag tctgaagcgg aagatcaaac tgaggcggag ataatagccg acaggataat
     3481 tggaaatagc cagaggatgg cgaacctcaa aattcgtcgt acaaagttca aaagtgtctt
     3541 gtatcatata ctaaaggaac taattcaatc tcagggaacc gtaaaggttt atcgcggtag
     3601 tagtttttca cacgattcga taaagataag cttacattat gaagagcagc atattacagc
     3661 cgtatgggtc tacttgacag taaaatttga agagcattgg aagcctgttg atgtagaggt
     3721 cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag
     3781 cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa
     3841 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
     3901 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
     3961 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
     4021 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
     4081 tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat
     4141 ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt
     4201 atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg
     4261 gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac cctagaagta
     4321 ttacgtgatt ttctgcccct taccctcgtt gctactctcc tttttttcgt gggaaccgct
     4381 ttagggccct cagtgatggt gttttgtaat ttatatgctc ctcttgcatt tgtgtctcta
     4441 cttcttgttc gcctggaggg aacttcttca tttgtattag catggttcac ttcagtcctt
     4501 ccttccaact cactcttttt ttgctgtaaa cgattctctg ccgccagttc attgaaacta
     4561 ttgaatatat cctttagaga ttccgggatg aataaatcac ctattaaagc agcttgacga
     4621 tctggtggaa ctaaagtaag caattgggta acgacgctta cgagcttcat aacatcttct
     4681 tccgttggag ctggtgggac taataactgt gtacaatcca tttttctcat gagcatttcg
     4741 gtagctctct tcttgtcttt ctcgggcaat cttcctatta ttatagcaat agatttgtat
     4801 agttgctttc tattgtctaa cagcttgtta ttctgtagca tcaaatctat ggcagcctga
     4861 cttgcttctt gtgaagagag cataccattt ccaatcgaat caaacctttc cttaaccatc
     4921 ttcgcagcag gcaaaattac ctcagcactg gagtcagaag atacgctgga atcttctgcg
     4981 ctagaatcaa gaccatacgg cctaccggtt gtgagagatt ccatgggcct tatgacatat
     5041 cctggaaaga gtagctcatc agacttacgt ttactctcta tatcaatatc tacatcagga
     5101 gcaatcattt caataaacag ccgacataca tcccagacgc tataagctgt acgtgctttt
     5161 accgtcagat tcttggctgt ttcaatgtcg tccattttgg ttttctttta ccagtattgt
     5221 tcgtttgata atgtattctt gcttattaca ttataaaatc tgtgcagatc acatgtcaaa
     5281 acaacttttt atcacaagat agtaccgcaa aacgaacctg cgggccgtct aaaaattaag
     5341 gaaaagcagc aaaggtgcat ttttaaaata tgaaatgaag ataccgcagt accaattatt
     5401 ttcgcagtac aaataatgcg cggccggtgc atttttcgaa agaacgcgag acaaacagga
     5461 caattaaagt tagtttttcg agttagcgtg tttgaatact gcaagataca agataaatag
     5521 agtagttgaa actagatatc aattgcacac aagatcggcg ctaagcatgc cacaatttgg
     5581 tatattatgt aaaacaccac ctaaggtgct tgttcgtcag tttgtggaaa ggtttgaaag
     5641 accttcaggt gagaaaatag cattatgtgc tgctgaacta acctatttat gttggatgat
     5701 tacacataac ggaacagcaa tcaagagagc cacattcatg agctataata ctatcataag
     5761 caattcgctg agtttcgata ttgtcaataa atcactccag tttaaataca agacgcaaaa
     5821 agcaacaatt ctggaagcct cattaaagaa attgattcct gcttgggaat ttacaattat
     5881 tccttactat ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt
     5941 acagttcgaa tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa
     6001 agcacttcta agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt
     6061 tgagtatact tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac
     6121 tttcatcaat tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt
     6181 agtccaaaat aagtatctgg gagtaataat ccagtgttta gtgacagaga caaagacaag
     6241 cgttagtagg cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt
     6301 ggatgaattt ttgaggaatt ctcatgtttg acagcttatc atcggacaat cttacatggt
     6361 ctacctttga tgacaacgaa accattcttt ctcaaggcag aacattgcat tgggtaggtg
     6421 gcggaggcac cagcgtcagc attttcaaag gttgtgttct tcgtcagaca tgttttagtg
     6481 tgtgaatgaa ataggtgtat gttttctttt gcagacaata attaggaaca aggtaaggga
     6541 actaaagtgt agaataagaa ttaaaaaaga agaacaagtt gaaaggcaag ttgaaatttc
     6601 aagaaaaaag tcaattgaag tacagtaaat tgacctgaat atatctgagt tccgagcaac
     6661 aatgagttta ccgaagagaa caatggaata ggaaactttg aacgaagaaa ggaaagcagg
     6721 aaaggaaaaa atttttaggc tcgagaacaa tagggcaaaa aaacaggcaa cgaacgaaca
     6781 aatggaaaaa cgaaaaaaaa aaaaacacag aaaagaatgc agaaagttgt aaactgaaaa
     6841 aaaaaaaaaa aaaaggtgaa cacaggaaaa aaaataaaaa aaaaaaaaaa ggaggacgaa
     6901 acaaaaaagt gaaaaaaaat gaaaattttt ttggaaaacc aagaaatgaa ttatatttcc
     6961 gtgtgagacg acatcgtcga atatgattca gggtaacagt atttatgtaa tcaatttcct
     7021 acctgaatct aaaattcccg ggagcaagat caagatgttt tcaccgatct ttccggtctc
     7081 tttggccggg gtttacggac gatgaccgaa gaccaagcgc cagctcattt ggcgagcgtt
     7141 ggttggtgga tcaagcccac gcgtaggcaa tcctcgagca gatccgccag gcgtgtatat
     7201 agcgtggatg gccaggcaac tttagtgctg acacatacag gcatatatat atgtgtgcga
     7261 cgacacatga tcatatggca tgcatgtgct ctgtatgtat ataaaactct tgttttcttc
     7321 ttttctagga ttttatcccc gctgccatca tggttcgacc attgaactgc atcgtcgccg
     7381 tgtcccaaaa tatggggatt ggcaagaacg gagacctacc ctggcctccg ctcaggaacg
     7441 agttcaagta cttccaaaga atgaccacaa cctcttcagt ggaaggtaaa cagaatctgg
     7501 tgattatggg taggaaaacc tggttctcca ttcctgagaa gaatcgacct ttaaaggaca
     7561 gaattaatat agttctcagt agagaactca aagaaccacc acgaggagct cattttcttg
     7621 ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc ggaattggca agtaaagtag
     7681 acatggtttg gatagtcgga ggcagttctg tttaccagga agccatgaat caaccaggcc
     7741 accttagact ctttgtgaca aggatcatgc aggaatttga aagtgacacg tttttcccag
     7801 aaattgattt ggggaaatat aaacttctcc cagaataccc aggcgtcctc tctgaggtcc
     7861 aggaggaaaa aggcatcaag tataagtttg aagtctacga gaagaaagac taacaggaag
     7921 atgctttcaa gttctctgct cccctcctaa agctatgcat ttttataaga ccatgggact
     7981 tttgctggct ttagatccgt cccccttttc ctttgtcgat atcatgtaat tagttatgtc
     8041 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
     8101 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
     8161 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
     8221 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agcttttcaa
     8281 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt
     8341 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
     8401 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
     8461 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
     8521 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
     8581 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
     8641 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
     8701 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
     8761 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
     8821 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
     8881 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
     8941 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
     9001 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
     9061 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
     9121 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
     9181 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
     9241 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     9301 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
     9361 ttatatcagt tattacccgg gaatctcggt cgtaatgatt tttataatga cgaaaaaaaa
     9421 aaaattggaa agaaaaagct tcgggccgcg ttgctggcgt ttttccatag gctccgcccc
     9481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10081 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10141 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10201 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10261 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10321 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10381 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10441 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10501 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
    10561 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10621 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10681 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10741 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10801 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
    10861 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10921 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10981 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11041 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11101 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11161 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    11221 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    11281 cttcaagaat tcccggggat cctctagagt cgacgggggg gggggggggg aaggaaaggg
    11341 aggaggagga gaaggaggtg gggaggaggt ttggagcacc gcaacagtgg taggtcgctt
    11401 gtggggaagc ctccaagacc ttagaaggga agataggatg gagctgggga gctggtggtg
    11461 gcgtctcagg acctttgtct ctaacactca ttcctgttga agctcttgac aatgggtgaa
    11521 gttgatgtct tgtgagtggc ctcacaggta tagctgttat gtcgttcata ctcgtccttg
    11581 gtcaacgtga gggtgctgct catgctgtag gtgctgtctt tgctgtcctg atcagtccaa
    11641 ctgttcagga cgccattttg tcgttcactc ccatcaatct tccacttgac attgatgtct
    11701 ttggggtaga agttgttcaa gaagcacacg actgaggcac ctccagatgt taactgctca
    11761 ctggatggtg ggaagatgga tacagttggt gcagcatcag cccgtttcat ttccaacttt
    11821 gtccccgagc cgaacgtgaa tggagtgtcc caaaaatgtt gacagtaata actcccaaaa
    11881 tcttcagact gcaggctgtt gatcttgagg gaatactgtg tgcctgatcc actgccactg
    11941 aaccttgatg gcacaccatc tactaacttt gtcgcaacat agacaaggag ctgaggagat
    12001 tttccctgtt tctgctgata ccatgctaaa ttactgtaaa tattctcact tgctcgacat
    12061 gtgatggtga cagattctcc cacagaaacg gatagggagg ctggagactg agtcatctgg
    12121 atgtcccttt tatccaaaga taccccttct tctttagcag caatgctggc aatagtagta
    12181 tttataaaca ataacccgtt atttgtgctg ttggaaaatg gcaaaacagc aacatcgaaa
    12241 tccccttcta aatctgagta accgatgaca gcttcagccg gaatttgtgc cgtttcatct
    12301 tctgttgtag tgttgactgg agcagctaat gcggaggatg ctgcgaataa aactgcagta
    12361 aaaattgaag gaaatctcat tctcgactct agaggatcac aatcacttct tctgaatgag
    12421 atttagtcac gatcgttttt tctccttgac gttaaagtat agaggtatat taacaatttt
    12481 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
    12541 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat
    12601 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta
    12661 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt
    12721 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg
    12781 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt
    12841 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtccggat ccgtccccct
    12901 tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca
    12961 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
    13021 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
    13081 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
    13141 gacgctcgaa ggctttaatt tgcaagctcg gaa
//
