LOCUS       40924_39033        7589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pSCUDMFE6L2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      27..1016
                     /label=CYC1 promoter
                     /note="CYC1 promoter"
                     /regulatory_class="promoter"
     CDS             1040..1600
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     terminator      1712..1959
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        1978..2389
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             2409..2789
                     /codon_start=1
                     /note="unnamed protein product; bleomycin"
                     /protein_id="SJL88495.1"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLKL
                     EFFAHPGLDPLASWFSCCLRLDDLAEFYRQCKSVGIQETSSGYPRIHAPELQEWGGTMA
                     ALVDPDGTLLRLIQNELLAGIS"
     regulatory      2888..3158
                     /label=CYC1 terminator
                     /note="CYC1 terminator"
                     /regulatory_class="terminator"
     terminator      2908..3155
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3182..3770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3944..4801)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4802..4906)
                     /label=AmpR promoter
     terminator      complement(5024..5271)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    complement(5330..5479)
                     /label=3' UTR of mIghg2b
                     /note="3' UTR of mIghg2b"
     CDS             complement(5483..5800)
                     /label=mIg-kappa-CL
                     /note="Mouse immunoglobulin kappa light chain constant
                     region"
     CDS             complement(6125..6379)
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     promoter        complement(6441..6882)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gap             6892..7192
                     /estimated_length=301
     misc_feature    7223..7561
                     /label=Ty1
                     /note="Ty1"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggaca atcttacatg gtctaccttt gatgacaacg
       61 aaaccattct ttctcaaggc agaacattgc attgggtagg tggcggaggc accagcgtca
      121 gcattttcaa aggttgtgtt cttcgtcaga catgttttag tgtgtgaatg aaataggtgt
      181 atgttttctt ttgcagacaa taattaggaa caaggtaagg gaactaaagt gtagaataag
      241 aattaaaaaa gaagaacaag ttgaaaggca agttgaaatt tcaagaaaaa agtcaattga
      301 agtacagtaa attgacctga atatatctga gttccgagca acaatgagtt taccgaagag
      361 aacaatggaa taggaaactt tgaacgaaga aaggaaagca ggaaaggaaa aaatttttag
      421 gctcgatcga gaacaatagg gcaaaaaaac aggcaacgaa cgaacaaatg gaaaaacgaa
      481 aaaaaaaaaa acacagaaaa gaatgcagaa agttgtaaac tgaaaaaaaa aaaaaaaaaa
      541 ggtgaacaca ggaaaaaaaa taaaaaaaaa aaaaaaggag gacgaaacaa aaaagtgaaa
      601 aaaaatgaaa atttttttgg aaaaccaaga aatgaattat atttccgtgt gagacgacat
      661 cgtcgaatat gattcagggt aacagtattt atgtaatcaa tttcctacct gaatctaaaa
      721 ttcccgggag caagatcaag atgttttcac cgatctttcc ggtctctttg gccggggttt
      781 acggacgatg accgaagacc aagcgccagc tcatttggcg agcgttggtt ggtggatcaa
      841 gcccacgcgt aggcaatcct cgatcgagca gatccgccag gcgtgtatat agcgtggatg
      901 gccaggcaac tttagtgctg acacatacag gcatatatat atgtgtgcga cgacacatga
      961 tcatatggca tgcatgtgct ctgtatgtat ataaaactct tgttttcttc ttttctagga
     1021 ttttatcccc gctgccatca tggttcgacc attgaactgc atcgtcgccg tgtcccaaaa
     1081 tatggggatt ggcaagaacg gagacctacc ctggcctccg ctcaggaacg agttcaagta
     1141 cttccaaaga atgaccacaa cctcttcagt ggaaggtaaa cagaatctgg tgattatggg
     1201 taggaaaacc tggttctcca ttcctgagaa gaatcgacct ttaaaggaca gaattaatat
     1261 agttctcagt agagaactca aagaaccacc acgaggagct cattttcttg ccaaaagttt
     1321 ggatgatgcc ttaagactta ttgaacaacc ggaattggca agtaaagtag acatggtttg
     1381 gatagtcgga ggcagttctg tttaccagga agccatgaat caaccaggcc accttagact
     1441 ctttgtgaca aggatcatgc aggaatttga aagtgacacg tttttcccag aaattgattt
     1501 ggggaaatat aaacttctcc cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa
     1561 aggcatcaag tataagtttg aagtctacga gaagaaagac taacaggaag atgctttcaa
     1621 gttctctgct cccctcctaa agctatgcat ttttataaga ccatgggact tttgctggct
     1681 ttagatccgt cccccttttc ctttgtcgat atcatgtaat tagttatgtc acgcttacat
     1741 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
     1801 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
     1861 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
     1921 cttgagaagg ttttgggacg ctcgaaggct ttaatttgca agcttccccg ggggatcgat
     1981 cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
     2041 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaattttccc
     2101 tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga
     2161 gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt
     2221 tttcttgaaa tttttttttt tagttttttt ctctttcagt gacctccatt gatatttaag
     2281 ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact
     2341 ttttttactt cttgttcatt agaaagaaag catagcaatc taatctaagg ggcgagctcg
     2401 aattcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccacgg
     2461 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     2521 tccagcgcgg ggatctcaag ctggagttct tcgcccaccc cgggctcgat cccctcgcga
     2581 gttggttcag ctgctgcctg aggctggacg acctcgcgga gttctaccgg cagtgcaaat
     2641 ccgtcggcat ccaggaaacc agcagcggct atccgcgcat ccatgccccc gaactgcagg
     2701 agtggggagg cacgatggcc gctttggtcg acccggacgg gacgctcctg cgcctgatac
     2761 agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc cgttttccgc ctgaggtcac
     2821 tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag ctgctcacca cccaggatcg
     2881 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
     2941 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     3001 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     3061 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     3121 agaaggtttt gggacgctcg aaggctttaa tttgcaagct tcgggccgcg ttgctggcgt
     3181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     4261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     4561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4981 gtatcacgag gccctttcgt cttcaagaat taattccgag cttgcaaatt aaagccttcg
     5041 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     5101 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     5161 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     5221 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa
     5281 aggaaaaggg ggacggatcc ctctagagtc gacggggggg ggggggggga aggaaaggga
     5341 ggaggaggag aaggaggtgg ggaggaggtt tggagcaccg caacagtggt aggtcgcttg
     5401 tggggaagcc tccaagacct tagaagggaa gataggatgg agctggggag ctggtggtgg
     5461 cgtctcagga cctttgtctc taacactcat tcctgttgaa gctcttgaca atgggtgaag
     5521 ttgatgtctt gtgagtggcc tcacaggtat agctgttatg tcgttcatac tcgtccttgg
     5581 tcaacgtgag ggtgctgctc atgctgtagg tgctgtcttt gctgtcctga tcagtccaac
     5641 tgttcaggac gccattttgt cgttcactcc catcaatctt ccacttgaca ttgatgtctt
     5701 tggggtagaa gttgttcaag aagcacacga ctgaggcacc tccagatgtt aactgctcac
     5761 tggatggtgg gaagatggat acagttggtg cagcatcagc ccgtttcatt tccaactttg
     5821 tccccgagcc gaacgtgaat ggagtgtccc aaaaatgttg acagtaataa ctcccaaaat
     5881 cttcagactg caggctgttg atcttgaggg aatactgtgt gcctgatcca ctgccactga
     5941 accttgatgg cacaccatct actaactttg tcgcaacata gacaaggagc tgaggagatt
     6001 ttccctgttt ctgctgatac catgctaaat tactgtaaat attctcactt gctcgacatg
     6061 tgatggtgac agattctccc acagaaacgg atagggaggc tggagactga gtcatctgga
     6121 tgtccctttt atccaaagat accccttctt ctttagcagc aatgctggca atagtagtat
     6181 ttataaacaa taacccgtta tttgtgctgt tggaaaatgg caaaacagca acatcgaaat
     6241 ccccttctaa atctgagtaa ccgatgacag cttcagccgg aatttgtgcc gtttcatctt
     6301 ctgttgtagt gttgactgga gcagctaatg cggaggatgc tgcgaataaa actgcagtaa
     6361 aaattgaagg aaatctcatt ctcgactcta gaggatcaca atcacttctt ctgaatgaga
     6421 tttagtcacg atcgtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt
     6481 tgttgatact tttattacat ttgaataaga agtaatacaa accgaaaatg ttgaaagtat
     6541 tagttaaagt ggttatgcag tttttgcatt tatatatctg ttaatagatc aaaaatcatc
     6601 gcttcgctga ttaattaccc cagaaataag gctaaaaaac taatcgcatt atcatcctat
     6661 ggttgttaat ttgattcgtt catttgaagg tttgtggggc caggttactg ccaatttttc
     6721 ctcttcataa ccataaaagc tagtattgta gaatctttat tgttcggagc agtgcggcgc
     6781 gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc
     6841 ttccttcgga gggctgtcac ccgctcggcg gcttctaatc cgtccggatc cnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttttttt
     7201 tctgtgaatg tacgtatatt tttgagatat atgtgggtaa ttagataatt gttgggattc
     7261 cattgttgat aaaggctata atattaggta tacagaatat actagaagtt ctcctcgagg
     7321 atataggaat cctcaaaaat ggaatctata ttttctacat actaatatta cgattattcc
     7381 tcattccgtt ttatatgttt atattcattg atcctataca ttatcaatcc ttgcgtttca
     7441 gcttcctcta acatcgatga cagcttctca taacttatgt catcatctta acaccgtata
     7501 tgataatata ttggtaatat aactattagt tgatagacga tagtggattt ttattccaac
     7561 ataccaccca taatgtaata gatccggaa
//
