LOCUS       40924_39018        8084 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning shuttle vector pSCR202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Genetic evidence of a major role for glucose-6-phosphate 
            dehydrogenase in nitrogen fixation and dark growth of the 
            cyanobacterium Nostoc sp. strain ATCC 29133
  JOURNAL   J. Bacteriol. 177 (21), 6184-6194 (1995)
  PUBMED    7592384
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   3  (bases 1 to 8084)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   4  (bases 1 to 8084)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1995"; volume: "177"; issue: "21"; pages: 
            "6184-6194"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2004"; volume: "59"; issue: "2"; pages: 
            "181-188"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St., 
            Northridge, CA 91330-8303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    212..268
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(281..297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(305..321)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(329..359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(374..395)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(683..1271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1445..2302)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2320..8084
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatcctc
      241 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      841 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
      901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1081 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2281 cacggaaatg ttgaatactc atactcttcc tttttcaatc gcgtctcgtg ccgattagta
     2341 tacttattgc cgatgcattt accaaaattt gcaaccggag aagttcactt gtccagtaaa
     2401 aacttccaaa aatttaccgg acaaccttat gttatccgcc agcaaatttt gacgcacagt
     2461 acgccagttt tggctgaaag tttcaactgt tgcgaaaagt gcctagtgaa ctcgttgtac
     2521 aaaaaaattt tcgcgtttgg catcaagcca cgcactctta aggttttgac aaaatatgtc
     2581 attccaagct tctgcaatgg aatttaaagt taatccccat ccgcgatatt tcggcactag
     2641 ggaagcagca tgatccatgc attggcgcag actgccagag catagctcat cacatgtcca
     2701 gactacctgg ttttcttcat ccaactcatg ttctaccagc atcttcaaaa cccacatctt
     2761 accccctcaa aaaaattctc aaaaatgtcc cgtcacttcc tggggcttgt gatgattgct
     2821 ttgcaatcgt ctgcctgcgg gacggctcga ttttcgtgaa actggcactg tgccagttta
     2881 caacgccact tccgccgctt tgcgcgcatg gttgttagct tgaattgctc gcggtaaatg
     2941 ttgcagcgag ccatccaggt tttacccttc atttggaaga aacgcccaga cagttccccg
     3001 ctggcagctg gtaagtgttc ggctgctgct ttctggctcc tggcaggatg ccatcagaaa
     3061 acgcgatcgc cccttcaccc aatgactgat acccaatgac tgatacccaa tgtctgactg
     3121 agtgccctcg ctactccaag cccagcactc agacgcttca ttgcttacag cccggtcaag
     3181 ttgagcgtat tcatacttcg cttggcacag ttgccttttc tccatgccga gcgttcgctg
     3241 atgccgccgc actgtgcgcg tgtggatgcc caatttacag ctgaccgcat tttgagaagt
     3301 cccgtaatga atgaagcttt tagaaacgaa cacccgcgac ggactgacat gaagaacaaa
     3361 aggtacttcc cccgctccca atttaaacga ggattgcccc tcatccccca acagttcgtt
     3421 tggatgtggc gctccgtaag acttgcgata ctccggtttc agtttgcggt tagccgcgta
     3481 tctacttctt tgttgcaaac gctgtgtgac tatgcccgtg gtcagcgctc gaatgttggc
     3541 gttgacctcc cataacggac attctgctac taccccccaa tcttttaaat ttaaatgcca
     3601 agccacctta gtcagtccgc ccaggtaaac cgtgagcagt ccaattctta ccttgtatcc
     3661 cgacaaaaat gcccctactg cttttccctg gtcaagccat ctgtacacag tggcttgact
     3721 aactcccaat atctcctcta ccagcacgag cggaatttct ttaacccgtc cgctcccatc
     3781 atcaaaaccg cgcaacaaat accacagccg cgcccaaggt tgacggctaa ttccactgtg
     3841 taaccgcacc atgaacggct gcaaaattat ctcggctttg actagtatca aaaccaacgg
     3901 gagtggtaca tttgaactta aggctgaacg atcctcctgt gaagtcaggg ggcaatggtt
     3961 cagcccctca acttgggtag ctgcgcgatt ttgattggcc actggttttc actcaaacct
     4021 ggttcaatgt gccaatcgct tcactaaatt taagttttcg cttccgctcg actgaatact
     4081 gtgctgatcc tcgaagggtg gtaatatgtt tgaagttcat tgcttctggg tgattaagga
     4141 atgattgccc agtagttttt ccaaatcttc accctatctc tacactgttt accgatccac
     4201 gcagttgtat accaacccca aaacttgata aattcaagtt aacttttgtt attgtctggt
     4261 ttttggatta cggacttgat gggcctggtg aacgactgct gcatggaact ctgtaaacat
     4321 gtgtagacga ctgggtaatc aatttctcca ctaccggact ttttcttata taagttttct
     4381 tgtgtaagat ttgcactttt agaagtatgc gtatcctatt aggaaaactc acagttttaa
     4441 gaaaaactgt atttagcata catcggttag agccgtgtat caattgacaa catcctatac
     4501 aggatctagg ttttgcacgt gccataaggt gacgaaatat ttatttcgtc acataaagct
     4561 tgaacagtaa tcactgcaag cagttgaaat tctacccgtg tagtatgggt atagaatttt
     4621 cctttttctg tttttttgta gtcaaaattt aggcaaaaca tgctactcat ttgctactca
     4681 aaatatgttt gaaaccattg attaatcgtt tatgccaaca tttcacccca aaatacacga
     4741 aatcttgtaa ctattgctat gactagcttt cacccgttgg tgtaatgatt ctgtagaatg
     4801 ttgtaccccc ttactggtaa acccttgctg tgactagctt tcatcaatga ccaatggatt
     4861 tcaagtccgc gtgtccttag ttatccccgc tacatctatc tgccattctg cttttcaagc
     4921 attaaaagca ttgtttccaa ttcttcccaa tgcggttgtg aaagagtttg gtagcactga
     4981 agcaacagtt cggaatgctc cggcgcatca tcagaagcta tgcttaacag cttgtggtat
     5041 tgtttaagct ggttaaattc atccaaggca cgggtaatca aaacctttgc agattcgttt
     5101 ctagtctgtg acatcatgtt ttgcatcccc gattacattg gttctacttg gcactcatcg
     5161 acccattcca cttcacggtc cgtctacttc aaaccaggga tgctcttctg gcaaggacac
     5221 cgcatattgc caaaaagcac cagtccaaaa aagcccaatc acctcaacag gatgcagaat
     5281 ttcccccctg gggactgtca tcctatgcag cacggtttgc ccgatatgat atccaggttt
     5341 actaaaagcc tttgtaatcc gcaaatgcca aaaactcagc gcgttgcatc gcagcagccc
     5401 gaaacgggcg cgtttaacgc taccattgct tattgcctcc ttagtgggct aacaactttt
     5461 aagggggaag tcgtgccggt gggggagatg tggtaatttc tcctgccggg gcgcatttaa
     5521 ggcctgactt catttgtcgc atcaggtatc ttgaattatt gaaccagatt tgagcgcaaa
     5581 aagcgatcgc gctgcggggc gattactgca tgttctagct tgagcagtat ttattctgct
     5641 agaactcttg agctacttta ttcgggacgg gagatcgttt aaccatccta tgtgggtagt
     5701 gattttcaag ggagcgagac gacgctacga tttactcgct acaaccttgt ttactatcgt
     5761 ctactttttg tctacaaaca gtctaaggta gtaaatcatg tattgtcaac attgtctaca
     5821 attttgtgga caatctctct aggttaacct agtatgtaga ctaggtgtat acaaatagtc
     5881 tacaaatgcg taacaatata atttcatttc gctttagtga cgctgaatta gaagcgttgc
     5941 aagcattcca aactccagaa gataaatccc ctagccaaat agccgcaaga ttagtaaggg
     6001 gaataattgc agggatggta gaaccgtcta caccgtctac acctgtagac atacaggaat
     6061 tagttaagca gcaagtcgag caggctatca ccgactcgca actgttgaaa gatatgattg
     6121 cgctcaatac ctctcaccta acaaaaggca tcgacaaaat taagcaggag gtcgatgaac
     6181 gtttggagga actgcgggga aaatcgaaag cccggtagca gagaaggata ctagcgctac
     6241 cgggcaagta atgaataata gcgacttaat cttagtccgt gatcgcgtcc ttgccaagct
     6301 gaaaatgggt aagcaatcaa ccgccgggaa ggcaatcgac gcatttatca aagaactgca
     6361 caaaactccg accgcaccgg aggaacaaaa gccgtctacc tgggaagccc gcgctgacaa
     6421 cttcctcaag gaggttgcag gttgcaggca atggctaacc accgaatcaa aatgcgcctc
     6481 atgggtacta aggaagattt ggagcgctgg ctgtggtttg tgggcaagat gcaagagcgg
     6541 ggactggcaa gcatcattga gaaaagttcg ccttacaaaa acagaggcga atcgctgcaa
     6601 caccgcgtct acttggaagt tgacttgctg ttggatgccc cagaagatga gattaagcct
     6661 ttataacccc agtacagtgc cacacatgga cggtttcaac ctgtctacag tccgtctaca
     6721 ccacaacaac acacgacgat cggtgcggtt acagtgcaac aatagacggt gacaagtgcg
     6781 gttgcagtgc aacaatagac ggtgacaagt gcggttgcag tgcaacaata gagcgtctac
     6841 acccaaacca gttaaaaaat tgcctgtaga cggctaaaaa ccgcttttga agagactttc
     6901 agcaactgta gacaaatttt tgggggagca tttttaacag aaatacccca aaagttagcc
     6961 atttttctta acagaatgct gatttttgta gactcgttcg gcttaacaaa atgctcaaaa
     7021 acatagcagc cgggtgcgaa cgtggttgca attctccaat gttctagctt taaatatcag
     7081 aatgtaacta gaactctgtg gaaccatgcc caaaaagaga tgcggactcg gctttgactg
     7141 cgggtcgatg atgttacaac ctgggataga ccccggcgac tgcgaaaatt acgccacctg
     7201 tggaggagcc acccaactca ccccagaaga agagtttgaa ttgatacgtt ggcgggaaat
     7261 tcaaaaccgg gaaaggcaag aggagtggga gcgccagcgc gaagcaattc gggtcaaccg
     7321 ccaccaagct gcattgatga tgctgatggg tcgcgcttgc ccccaaacgc ccgaatcatt
     7381 gggtgttgtg gatttgttag cccaagttgc aaccttagtt gaggaagtca gatcgcaact
     7441 gccccagttt gaagggcagt acatcgcacc tgaaaagtgc gaagcccaca cttacaacgt
     7501 caaacgaccc aaaggtgtat attgctacaa taagctgacc gctgccgagg caatttttga
     7561 gccgagcgag aaaagcgata aagtccgagt catccacctc agccacgacg gcgacccccg
     7621 cgactcagag gcgcggctgg gcatcgaacg ccgaaacgcc ttgactcaag ccaggactca
     7681 gatcaacgtt gcccaagttg cactcgctca agccttggag ttgctgcaat gttctagcag
     7741 aataaataat cagcaagcta gaacactcgc gcactcgccc ccatccagtg acgaaacacc
     7801 gtgaaaggcg atcgccccag gtgcatcaaa atttgcaaag tgttttattg caaatcttga
     7861 tgcagccacg cactgcatca ctcaccagct tccagcttcc gacttccact tacttcaact
     7921 agctactcta actggaagtt gaagttgtaa gttaccttgc gggtctaatt ttcctattct
     7981 gattcgctag attcttcttt tagactttca tcgtatgttt gataaacctg gatgcaacgt
     8041 cctggtttac gctcaaagct ttccgccgat ataacaaaag aatt
//
