LOCUS       40924_39013        8786 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning shuttle vector pSCR119, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Genetic evidence of a major role for glucose-6-phosphate 
            dehydrogenase in nitrogen fixation and dark growth of the 
            cyanobacterium Nostoc sp. strain ATCC 29133
  JOURNAL   J. Bacteriol. 177 (21), 6184-6194 (1995)
  PUBMED    7592384
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Argueta C, Yuksek K, Summers M.
  TITLE     Construction and use of GFP reporter vectors for analysis of 
            cell-type-specific gene expression in Nostoc punctiforme
  JOURNAL   J. Microbiol. Methods 59 (2), 181-188 (2004)
  PUBMED    15369854
REFERENCE   3  (bases 1 to 8786)
  AUTHORS   Summers ML, Wallis JG, Campbell EL, Meeks JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St.,
            Northridge, CA 91330-8303, USA
REFERENCE   4  (bases 1 to 8786)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1995"; volume: "177"; issue: "21"; pages: 
            "6184-6194"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods"; date: "2004"; volume: "59"; issue: "2"; pages: 
            "181-188"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2004) Biology, CSU Northridge, 18111 Nordhoff St., 
            Northridge, CA 91330-8303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    212..268
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(281..297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(305..321)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(329..359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(374..395)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(683..1271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1773..2564)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      2926..8786
                     /label=pDC1 cyanobacterial origin of replication
                     /note="pDC1 cyanobacterial origin of replication"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatcctc
      241 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1081 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1561 ccagtgctgc aatgataccg cgagacccac gctcaccggg tgggcgaaga actccagcat
     1621 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     1681 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     1741 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     1801 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     1861 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     1921 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     1981 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catccgcgcc
     2041 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     2101 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     2161 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     2221 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     2281 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     2341 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca
     2401 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     2461 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     2521 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     2581 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     2641 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     2701 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     2761 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     2821 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     2881 cagcccttgc gccctgagtg cttgcggcag cgtgaagcta gcttaaacgc tcaaattgca
     2941 gctttctagc cacgctcctg taaaaaatat atttctgaac agttaacctg tcagaagtac
     3001 attctctact tttaacggtc acgcgtctcg tgccgattag tatacttatt gccgatgcat
     3061 ttaccaaaat ttgcaaccgg agaagttcac ttgtccagta aaaacttcca aaaatttacc
     3121 ggacaacctt atgttatccg ccagcaaatt ttgacgcaca gtacgccagt tttggctgaa
     3181 agtttcaact gttgcgaaaa gtgcctagtg aactcgttgt acaaaaaaat tttcgcgttt
     3241 ggcatcaagc cacgcactct taaggttttg acaaaatatg tcattccaag cttctgcaat
     3301 ggaatttaaa gttaatcccc atccgcgata tttcggcact agggaagcag catgatccat
     3361 gcattggcgc agactgccag agcatagctc atcacatgtc cagactacct ggttttcttc
     3421 atccaactca tgttctacca gcatcttcaa aacccacatc ttaccccctc aaaaaaattc
     3481 tcaaaaatgt cccgtcactt cctggggctt gtgatgattg ctttgcaatc gtctgcctgc
     3541 gggacggctc gattttcgtg aaactggcac tgtgccagtt tacaacgcca cttccgccgc
     3601 tttgcgcgca tggttgttag cttgaattgc tcgcggtaaa tgttgcagcg agccatccag
     3661 gttttaccct tcatttggaa gaaacgccca gacagttccc cgctggcagc tggtaagtgt
     3721 tcggctgctg ctttctggct cctggcagga tgccatcaga aaacgcgatc gccccttcac
     3781 ccaatgactg atacccaatg actgataccc aatgtctgac tgagtgccct cgctactcca
     3841 agcccagcac tcagacgctt cattgcttac agcccggtca agttgagcgt attcatactt
     3901 cgcttggcac agttgccttt tctccatgcc gagcgttcgc tgatgccgcc gcactgtgcg
     3961 cgtgtggatg cccaatttac agctgaccgc attttgagaa gtcccgtaat gaatgaagct
     4021 tttagaaacg aacacccgcg acggactgac atgaagaaca aaaggtactt cccccgctcc
     4081 caatttaaac gaggattgcc cctcatcccc caacagttcg tttggatgtg gcgctccgta
     4141 agacttgcga tactccggtt tcagtttgcg gttagccgcg tatctacttc tttgttgcaa
     4201 acgctgtgtg actatgcccg tggtcagcgc tcgaatgttg gcgttgacct cccataacgg
     4261 acattctgct actacccccc aatcttttaa atttaaatgc caagccacct tagtcagtcc
     4321 gcccaggtaa accgtgagca gtccaattct taccttgtat cccgacaaaa atgcccctac
     4381 tgcttttccc tggtcaagcc atctgtacac agtggcttga ctaactccca atatctcctc
     4441 taccagcacg agcggaattt ctttaacccg tccgctccca tcatcaaaac cgcgcaacaa
     4501 ataccacagc cgcgcccaag gttgacggct aattccactg tgtaaccgca ccatgaacgg
     4561 ctgcaaaatt atctcggctt tgactagtat caaaaccaac gggagtggta catttgaact
     4621 taaggctgaa cgatcctcct gtgaagtcag ggggcaatgg ttcagcccct caacttgggt
     4681 agctgcgcga ttttgattgg ccactggttt tcactcaaac ctggttcaat gtgccaatcg
     4741 cttcactaaa tttaagtttt cgcttccgct cgactgaata ctgtgctgat cctcgaaggg
     4801 tggtaatatg tttgaagttc attgcttctg ggtgattaag gaatgattgc ccagtagttt
     4861 ttccaaatct tcaccctatc tctacactgt ttaccgatcc acgcagttgt ataccaaccc
     4921 caaaacttga taaattcaag ttaacttttg ttattgtctg gtttttggat tacggacttg
     4981 atgggcctgg tgaacgactg ctgcatggaa ctctgtaaac atgtgtagac gactgggtaa
     5041 tcaatttctc cactaccgga ctttttctta tataagtttt cttgtgtaag atttgcactt
     5101 ttagaagtat gcgtatccta ttaggaaaac tcacagtttt aagaaaaact gtatttagca
     5161 tacatcggtt agagccgtgt atcaattgac aacatcctat acaggatcta ggttttgcac
     5221 gtgccataag gtgacgaaat atttatttcg tcacataaag cttgaacagt aatcactgca
     5281 agcagttgaa attctacccg tgtagtatgg gtatagaatt ttcctttttc tgtttttttg
     5341 tagtcaaaat ttaggcaaaa catgctactc atttgctact caaaatatgt ttgaaaccat
     5401 tgattaatcg tttatgccaa catttcaccc caaaatacac gaaatcttgt aactattgct
     5461 atgactagct ttcacccgtt ggtgtaatga ttctgtagaa tgttgtaccc ccttactggt
     5521 aaacccttgc tgtgactagc tttcatcaat gaccaatgga tttcaagtcc gcgtgtcctt
     5581 agttatcccc gctacatcta tctgccattc tgcttttcaa gcattaaaag cattgtttcc
     5641 aattcttccc aatgcggttg tgaaagagtt tggtagcact gaagcaacag ttcggaatgc
     5701 tccggcgcat catcagaagc tatgcttaac agcttgtggt attgtttaag ctggttaaat
     5761 tcatccaagg cacgggtaat caaaaccttt gcagattcgt ttctagtctg tgacatcatg
     5821 ttttgcatcc ccgattacat tggttctact tggcactcat cgacccattc cacttcacgg
     5881 tccgtctact tcaaaccagg gatgctcttc tggcaaggac accgcatatt gccaaaaagc
     5941 accagtccaa aaaagcccaa tcacctcaac aggatgcaga atttcccccc tggggactgt
     6001 catcctatgc agcacggttt gcccgatatg atatccaggt ttactaaaag cctttgtaat
     6061 ccgcaaatgc caaaaactca gcgcgttgca tcgcagcagc ccgaaacggg cgcgtttaac
     6121 gctaccattg cttattgcct ccttagtggg ctaacaactt ttaaggggga agtcgtgccg
     6181 gtgggggaga tgtggtaatt tctcctgccg gggcgcattt aaggcctgac ttcatttgtc
     6241 gcatcaggta tcttgaatta ttgaaccaga tttgagcgca aaaagcgatc gcgctgcggg
     6301 gcgattactg catgttctag cttgagcagt atttattctg ctagaactct tgagctactt
     6361 tattcgggac gggagatcgt ttaaccatcc tatgtgggta gtgattttca agggagcgag
     6421 acgacgctac gatttactcg ctacaacctt gtttactatc gtctactttt tgtctacaaa
     6481 cagtctaagg tagtaaatca tgtattgtca acattgtcta caattttgtg gacaatctct
     6541 ctaggttaac ctagtatgta gactaggtgt atacaaatag tctacaaatg cgtaacaata
     6601 taatttcatt tcgctttagt gacgctgaat tagaagcgtt gcaagcattc caaactccag
     6661 aagataaatc ccctagccaa atagccgcaa gattagtaag gggaataatt gcagggatgg
     6721 tagaaccgtc tacaccgtct acacctgtag acatacagga attagttaag cagcaagtcg
     6781 agcaggctat caccgactcg caactgttga aagatatgat tgcgctcaat acctctcacc
     6841 taacaaaagg catcgacaaa attaagcagg aggtcgatga acgtttggag gaactgcggg
     6901 gaaaatcgaa agcccggtag cagagaagga tactagcgct accgggcaag taatgaataa
     6961 tagcgactta atcttagtcc gtgatcgcgt ccttgccaag ctgaaaatgg gtaagcaatc
     7021 aaccgccggg aaggcaatcg acgcatttat caaagaactg cacaaaactc cgaccgcacc
     7081 ggaggaacaa aagccgtcta cctgggaagc ccgcgctgac aacttcctca aggaggttgc
     7141 aggttgcagg caatggctaa ccaccgaatc aaaatgcgcc tcatgggtac taaggaagat
     7201 ttggagcgct ggctgtggtt tgtgggcaag atgcaagagc ggggactggc aagcatcatt
     7261 gagaaaagtt cgccttacaa aaacagaggc gaatcgctgc aacaccgcgt ctacttggaa
     7321 gttgacttgc tgttggatgc cccagaagat gagattaagc ctttataacc ccagtacagt
     7381 gccacacatg gacggtttca acctgtctac agtccgtcta caccacaaca acacacgacg
     7441 atcggtgcgg ttacagtgca acaatagacg gtgacaagtg cggttgcagt gcaacaatag
     7501 acggtgacaa gtgcggttgc agtgcaacaa tagagcgtct acacccaaac cagttaaaaa
     7561 attgcctgta gacggctaaa aaccgctttt gaagagactt tcagcaactg tagacaaatt
     7621 tttgggggag catttttaac agaaataccc caaaagttag ccatttttct taacagaatg
     7681 ctgatttttg tagactcgtt cggcttaaca aaatgctcaa aaacatagca gccgggtgcg
     7741 aacgtggttg caattctcca atgttctagc tttaaatatc agaatgtaac tagaactctg
     7801 tggaaccatg cccaaaaaga gatgcggact cggctttgac tgcgggtcga tgatgttaca
     7861 acctgggata gaccccggcg actgcgaaaa ttacgccacc tgtggaggag ccacccaact
     7921 caccccagaa gaagagtttg aattgatacg ttggcgggaa attcaaaacc gggaaaggca
     7981 agaggagtgg gagcgccagc gcgaagcaat tcgggtcaac cgccaccaag ctgcattgat
     8041 gatgctgatg ggtcgcgctt gcccccaaac gcccgaatca ttgggtgttg tggatttgtt
     8101 agcccaagtt gcaaccttag ttgaggaagt cagatcgcaa ctgccccagt ttgaagggca
     8161 gtacatcgca cctgaaaagt gcgaagccca cacttacaac gtcaaacgac ccaaaggtgt
     8221 atattgctac aataagctga ccgctgccga ggcaattttt gagccgagcg agaaaagcga
     8281 taaagtccga gtcatccacc tcagccacga cggcgacccc cgcgactcag aggcgcggct
     8341 gggcatcgaa cgccgaaacg ccttgactca agccaggact cagatcaacg ttgcccaagt
     8401 tgcactcgct caagccttgg agttgctgca atgttctagc agaataaata atcagcaagc
     8461 tagaacactc gcgcactcgc ccccatccag tgacgaaaca ccgtgaaagg cgatcgcccc
     8521 aggtgcatca aaatttgcaa agtgttttat tgcaaatctt gatgcagcca cgcactgcat
     8581 cactcaccag cttccagctt ccgacttcca cttacttcaa ctagctactc taactggaag
     8641 ttgaagttgt aagttacctt gcgggtctaa ttttcctatt ctgattcgct agattcttct
     8701 tttagacttt catcgtatgt ttgataaacc tggatgcaac gtcctggttt acgctcaaag
     8761 ctttccgccg atataacaaa agaatt
//
