LOCUS       V003410                13444 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003410
VERSION     V003410
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..282
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(696..1209)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1697..1713
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1980..3071)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     rep_origin      complement(3445..4775)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(4784..5031)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     gap             5102..5360
                     /estimated_length=259
     CDS             complement(8031..9248)
                     /gene="ST6GAL1"
                     /label="Beta-galactoside alpha-2,6-sialyltransferase 1"
                     /note="Beta-galactoside alpha-2,6-sialyltransferase 1 from
                     Homo sapiens. Accession#: P15907"
     protein_bind    9805..9922
                     /label="UAS"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             complement(10182..10982)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(10983..11199)
                     /label="URA3 promoter"
     primer_bind     complement(11219..11235)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    11243..11259
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11267..11297)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11312..11333)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11621..12209)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12382..13239)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13240..13344)
                     /label="AmpR promoter"
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg
     4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa
     5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg
     5101 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn gatatctcaa ctccaaacca atagcaatag cttatttgat
     5401 tggtggaagg attagagagg gtcccatgat ccagatgaat agatggctat caaagaagac
     5461 agaaccccat agacacaggc agcattaact cataattttt aaattaactg tcacaaatat
     5521 gaaacatctc accagaccaa tgataacgtc catatcataa tttgcatttt accaaagtgt
     5581 ccacatagac agcacagaac taaaagttct gtagacttca gaatcatatt taaattaatt
     5641 tgatccttta tattaataat aattaaaaac tagattctac ccagtgtcgt cccagtcttc
     5701 acaccaacat gtatgtcctg ctgtcggaat gccacctcct ctgcactcag tgtcatgatt
     5761 cgaagattaa aatcagtgtg tcaaatcaga acataaacca agccaggaat tcatnggccc
     5821 agagaggggt aaggggcttg tcctatacag cagttaagca gcccacgtgc tccgcccatt
     5881 cccaagcaga atcagattgg agctgcaggc agcgaggagg agggcgtgtt ctgtgttgtc
     5941 cactgctttg catggtttct tgggtgttcc aaggaggaca gttgttgtct cccaataaga
     6001 cagttaattc cctgaggaca ggacctcttc cacactagcc tccgtcacag cagggtgcat
     6061 gcagccgagg tgtgagcaca gagcaggtcc ctactaaagg caggaaggaa accagcatga
     6121 actgagcacc tactacgtgc caggtacaga gccaggacag gtgctttcct gtaatcctca
     6181 caaaagcagt gagataggat catcatcccc actttacagg tgtggaaaca ggctcaagga
     6241 agagcatttg cccagagtgg cacagctagt cagtagggga gcatcaattt gaaaatccag
     6301 gctttctcac tccanggccc tttctagcta cataagctga gggtttggca gtaaatgagg
     6361 agcccagctc cgagatggtt agtttggagc ccaaacatta gatccccttg agcacatggg
     6421 gccagccttg agagtgcaga gccctgggtc taggaatcct ggctctacag catgacttgg
     6481 tgatcctgtg caagtctttt aacctctcca caccagggct gcgctggtcc agcaggattc
     6541 cctatcaggg agaggggact tagtaaaacc atctggagag catgtctgca aactacagtg
     6601 ggccagcctc tgcccagaga gcctgaagtt gctcacttgc aaggaagagg tgcagagcat
     6661 tgcaatctgc taccacacct ctcacacctg gtgagaatca gttctgatct ccaaggttct
     6721 gacctctgag agtgacgtta agtccagaga gccacaccac tacaccctga gagaccttca
     6781 gcaaaaggcc cgtgagctca aagaaattgg ggtcaggatg cttttaaagc aaaaaatgct
     6841 ttaaaaaaaa aaaacagatg gtggttagga gaagatttgg gggtaggtat tgaaaaggaa
     6901 gaatgggact ggtatgtaaa cccagctgac aggacagggt caggccaggc agagcctgga
     6961 ggaatcacgt tttgtggctc tggattgcct ggtatctgtc gtgaggctgc acttgctacc
     7021 tcagacatgg gtaggaggtt tcctaaaaaa atatcttgag tctctaagtt caggtagatc
     7081 ttagaaaata aacatgggaa gaaagaaaaa cacaattcaa acggctcaga tgtaatctgc
     7141 ttgccatgtt tcttctcttc ctctaagact catctgaatg ccccctgtgc tataaagcct
     7201 tctctgatcc acaaacacag ttttaagtgc gtggccctcc tgggtgcaca gagagagcaa
     7261 gcccatctct ctccaaatcc ccagtgcact gagcacactg aatcctacct gcctgttcag
     7321 atgtctgcct ctctcactga accgtagcaa cttgaggata gacactgtat ccttctctac
     7381 cttccatctc cacatggaga gggaaagtgg gctaaccaag accctttaat cataagcacc
     7441 cagtctaagg accagcccaa ccagccagag tgataggaac acgttccagg ggcatggacc
     7501 tcgggagcaa cttttctctg gaggcgcgga gcatgtttcc aatcccctgt accacctact
     7561 tgccccccag agccaccctg cattgggcac aattgtaatt taagagaatt tctttaaaac
     7621 gtgaaatcaa tatttagatg cagacttcaa gcaatataga gcattggttt gtgagaatgg
     7681 catcattctg ctgggaaaaa caaagcccag ctttggcagc cctgatggga gagatagacc
     7741 ttgcatctac caaggtggaa ggcggggagg atgctgtctg gaaagaatgc tagatatgtg
     7801 tgcatgtgtg tggcaggagg ctctgggctc ataaactgtc tggctacagg gaaggctgga
     7861 cctgatttcc tgaccacagg ctcttgaagt ccccatggtc ttgctgacag aggcccctag
     7921 agtaaaagga gcaggctgga attgttcagg aaaatgttct tcccaggctg gggtggccaa
     7981 gagaccattc atttaatgcc tgatggagaa gagtgaggag cctgtgctta gcagtgaatg
     8041 gtccggaagc caggcagtgt ggcttttcca agcaggtaga tgtcctcatc tgtgccctgg
     8101 ttgagatgct tcaccaaatt cttctcatag agcagcgggt ggtaggcacc catcgtgcag
     8161 gcactatcga agaacttctg gtagtagtag cacacgtcag tcttgcgctt ggatgggagg
     8221 aactcataaa tatccacctg gtcacacagc gtcatcatga tgatgatacc aagcatccca
     8281 gaggatgggg ggtttggctg aatctcttct ggggagattt cttgaagaat gtcccatagc
     8341 tcccaaggca tctggggctt gaggatgtaa aagggctgat tggggtgcag cttacgataa
     8401 gtcttgtagt tgttaaagaa attataatcc ggattctggt accactttgg gatatctgag
     8461 tggtatacag atgggtccca tacaattagg attccttcat tgtacaaact gtctttgagg
     8521 aagcgcttct ctgtggtaac caactgagag ttcatcaggc gaatggtagt ttttgtgccc
     8581 acatcttgtt ggaagttggc tgtgggtgcc ccattaaacc tcaggactgc gtcatgatca
     8641 tcgatttctc tgcctagttg ggaggacttc agagatcccg ctgacgacac aacagcacac
     8701 ctgccccaag gcccagcctt ggtcctaatg ctctccttgg gcagataacc ctcccattca
     8761 gaggtattga agggaaaatc tgtgacctct accatggata cattcacatg gtcccggagg
     8821 tggcagcgca gggcctctgc actgaacttg atgcctggtc ctggcccctt gtaggacact
     8881 ttgtacttgt tcatgcttag gtaattcttc cagatctttt gcagcctagg gataaggttt
     8941 ttggaagagc tgtccttgtt ccacacctgg aaggaggcct ctggtttggc cttggctagg
     9001 cctctgagac tgccgagggt ctggcggccc ctgtgggggt cctgggtgct gcttgaggat
     9061 acagactggg aatcagaccc catggccaat ttccccagac tctttaacac ctggaattcc
     9121 ttggtttgca atttaaagga atcatagtaa ctccctttct tcttttcctt ccacacacag
     9181 atgactgcaa acagaagaaa gaccaggacg cagcagctga actttttctt caggttggtg
     9241 tgaatcataa tgaagatgtg ttcagggaag tcactttgtt ctgagcagga aaacaagcct
     9301 gcttctggct aatcccccag gggcggcagg cccttcaggt cctcaggaat tagcactatt
     9361 atccatggga gggaaggttt attgggtctg caggccaagg cccatttttc tcaggatgat
     9421 cgttggcctt tttggccaag ctgactctag aggatccccg ggtaccgagc tcgaattgat
     9481 ccggtaattt agtgtgtgta tttgtgtttg cgtgtctata gaagtatagt aatttatgct
     9541 acaaaggacc taatgtataa ggaaagaata tttagagaaa agaagaaaac aagagtttta
     9601 tatacataca gagcacatgc atgccatatg atcatgtgtc gtcgcacaca tatatatatg
     9661 cctgtatgtg tcagcactaa agttgcctgg ccatccacgc tatatacacg cctggcggat
     9721 ctgctcgagg tcgattagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta
     9781 actgctcatt gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg
     9841 aaggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
     9901 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
     9961 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta
    10021 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
    10081 cgaagcgatg atttttgatc gggtaataac tgatataatt aaattgaagc tctaatttgt
    10141 gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt
    10201 ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt
    10261 ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca
    10321 tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt
    10381 gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag
    10441 ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca
    10501 gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt
    10561 gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt
    10621 gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg
    10681 actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg
    10741 gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca
    10801 tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg
    10861 gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat
    10921 agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac
    10981 atgatttatc ttcgtttccg gtttttgttc tgtgcagttg ggttaagaat actgggcaat
    11041 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
    11101 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaaaga aaccgaaatc
    11161 aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa agctaattcg taatcatggt
    11221 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11281 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt
    11341 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccanctggat taatgaatcg
    11401 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    11701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    11761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    11821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    11881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    11941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12061 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12241 acgctcagtg gaacgaaaac tcacgttagg gattttggtc atgagattat caaaaaggat
    12301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    12361 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12421 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12481 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12541 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12601 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12661 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12721 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12781 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12841 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12901 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    12961 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13021 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    13081 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    13141 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    13201 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    13261 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    13321 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    13381 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    13441 tcaa
//
