LOCUS       40924_38993       10533 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pSCGALCaMET15-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10533)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(696..1209)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1697..1713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1980..3071)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     rep_origin      complement(3429..4775)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(4784..5031)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    complement(5135..5172)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(5173..6495)
                     /codon_start=1
                     /note="unnamed protein product; CaMET15"
                     /protein_id="SJL88109.1"
                     /translation="MPSHFDTLQLHAGQPVEKPHQPRAPPIYATTSYVFNDSKHGAQLF
                     GLETPGYIYSRIMNPTNDVFEQRIAALEGGIGALATSSGQSAQFLAIAGLAHAGDNIIS
                     TSYLYGGTYNQFKVAFKRLGIETKFVNGDAAEDFAKLIDDKTKAIYIETIGNPKYNVPD
                     FEKITKLAHEHGIPVVVDNTFGAGGFLVNPIAHGADIVVHSATKWIGGHGTTIAGVIVD
                     SGNFPWTEYPEKYPQFSKPSEGYHGLILNDALGKAAYIGHLRIELLRDLGPALNPFGSF
                     LLLQGLETLSLRVERQSENALKLAQWLEKNPNVESVSYLGLPSHESHELSKKYLNNDAK
                     YFGGALAFTVKDITNTSSDPFNEASPKLVDNLEIASNLANVGDSKTLVIAPWFTTHQQL
                     SDEEKLASGVTKGLIRVSTGTEYIDDIINDFEQAFKKVYNN"
     misc_feature    complement(6496..6511)
                     /label=5UTR
                     /note="5UTR"
     protein_bind    6894..7011
                     /label=UAS
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             complement(7271..8071)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(8072..8288)
                     /label=URA3 promoter
     primer_bind     complement(8308..8324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8332..8348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8356..8386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8401..8422)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8710..9298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9471..10328)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10329..10433)
                     /label=AmpR promoter
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg
     4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa
     5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg
     5101 cttttttttt tttttttttt ttnnaatgtg tctaattatt gttgtgaaac gttatcttat
     5161 ataccaatct atttagttgt tataaacctt cttgaatgct tgttcaaagt cgttaataat
     5221 atcatcaata tattcagtac cagtagaaac tctgattaag cccttggtaa caccagaagc
     5281 caacttttct tcatcagaca attgttgatg tgtagtaaac catggagcaa taaccaaagt
     5341 cttagagtca cccacattag ccaagtttga agcaatctcc aaattgtcaa ccaactttgg
     5401 tgaggcttca ttgaatgggt cgctggaggt gttggtgatg tccttgacag taaatgctaa
     5461 agcaccacca aagtacttag cgtcattgtt caagtatttt ttactcaatt cgtgggattc
     5521 gtgagatggc aatcccaaat aggacacaga ctcaacattt gggttctttt ccaaccattg
     5581 ggccaatttc aaagcatttt cagattgtct ttcaactctc aaagacaaag tttctaaacc
     5641 ttgcaacaac aaaaaacttc caaatggatt caaagctgga cccaagtctc tcaacaattc
     5701 aattctcaag tgaccaatgt atgcggcctt acctaaagca tcattcaaga tcaacccgtg
     5761 gtaaccttct gatggtttag agaattgtgg gtatttttct gggtactcgg tccatgggaa
     5821 gttaccggaa tcaacaataa caccagcaat tgtagtaccg tgaccaccaa tccatttagt
     5881 agcagaatga acaacaatat cagcaccgtg ggcaattggg ttaactaaaa atccaccagc
     5941 accaaaagtg ttgtcgacaa caacaggaat accgtgttca tgggccaatt tggtgatttt
     6001 ttcaaagtcc ggaacattat atttagggtt tccaatggtt tcaatataaa tagcttttgt
     6061 cttgtcgtca atcaatttag caaaatcttc agcggcgtca ccattaacga atttggtttc
     6121 aatgcccaaa cgtttgaaag caactttgaa ttgattataa gtaccaccat acaagtagga
     6181 tgtactgata atgttatcac cagcatgagc caacccagca atggccaaga attgagctga
     6241 ttgaccagaa gaagtggcca atgcaccaat accaccttcc aaggcagcaa ttctttgttc
     6301 aaacacatcg tttgttggat tcataattct ggagtaaatg tatcctgggg tttctaaacc
     6361 aaataattga gcaccgtgtt tagagtcatt gaaaacatag gaggtggttg cataaattgg
     6421 tggggctctt ggttggtgtg gtttttcaac tggttgacca gcatgtaatt gaagtgtatc
     6481 aaagtgagaa ggcattgtag tttagtaatg gttggccttt ttggccaagc tgactctaga
     6541 ggatccccgg gtaccgagct cgaattgatc cggtaattta gtgtgtgtat ttgtgtttgc
     6601 gtgtctatag aagtatagta atttatgcta caaaggacct aatgtataag gaaagaatat
     6661 ttagagaaaa gaagaaaaca agagttttat atacatacag agcacatgca tgccatatga
     6721 tcatgtgtcg tcgcacacat atatatatgc ctgtatgtgt cagcactaaa gttgcctggc
     6781 catccacgct atatacacgc ctggcggatc tgctcgaggt cgattagcct aaaaaaacct
     6841 tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa gtacggatta
     6901 gaagccgccg agcgggtgac agccctccga aggaagactc tcctccgtgc gtcctcgtct
     6961 tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa
     7021 gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc
     7081 acaaaccttc aaatgaacga atcaaattaa caaccatagg atgataatgc gattagtttt
     7141 ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatcg ggtaataact
     7201 gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata
     7261 cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt
     7321 aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa
     7381 taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt
     7441 ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc
     7501 ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa
     7561 tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat gacaattctg
     7621 ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc
     7681 taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc
     7741 tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt
     7801 cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct
     7861 ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta
     7921 atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt
     7981 ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag
     8041 cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttccgg tttttgttct
     8101 gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat
     8161 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
     8221 atcaaaaaaa tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
     8281 gaattgaaaa gctaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8341 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8401 atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8461 cctgtcgtgc canctggatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9121 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaggg
     9361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10441 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10501 aataggcgta tcacgaggcc ctttcgtctt caa
//
