LOCUS       40924_38988       10554 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pSCGALCaMET15-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10554)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(696..1209)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1697..1713
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1980..3071)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     rep_origin      complement(3429..4775)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(4784..5031)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    complement(5113..5170)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(5171..6493)
                     /codon_start=1
                     /note="unnamed protein product; CaMET"
                     /protein_id="SJL88105.1"
                     /translation="MPSHFDTLQLHAGQPVEKPHQPRAPPIYATTSYVFNDSKHGAQLF
                     GLETPGYIYSRIMNPTNDVFEQRIAALEGGIGALATSSGQSAQFLAIAGLAHAGDNIIS
                     TSYLYGGTYNQFKVAFKRLGIETKFVNGDAAEDFAKLIDDKTKAIYIETIGNPKYNVPD
                     FEKITKLAHEHGIPVVVDNTFGAGGFLVNPIAHGADIVVHSATKWIGGHGTTIAGVIVD
                     SGNFPWTEYPEKYPQFSKPSEGYHGLVLNDALGKAAYIGHLRIELLRDLGPALNPFGSF
                     LLLQGLETLSLRVERQSENALKLAQWLEKNPNVESVSYLGLPSHESHELSKKYLNNDAK
                     YFGGALAFTVKDITNTSSDPFNEASPKLVDNLEIASNLANVGDSKTLVIAPWFTTHQQL
                     SDEEKLASGVTKGLIRVSTGTEYIDDIINDFEQAFKKVYNN"
     misc_feature    complement(6494..6531)
                     /label=5UTR
                     /note="5UTR"
     protein_bind    6915..7032
                     /label=UAS
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             complement(7292..8092)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(8093..8309)
                     /label=URA3 promoter
     primer_bind     complement(8329..8345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8353..8369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8377..8407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8422..8443)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8731..9319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9492..10349)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10350..10454)
                     /label=AmpR promoter
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg
     4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa
     5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg
     5101 cttttttttt ttttttaaca ttaatgtgtc taattattgt tgtgaaacgt tatcttatat
     5161 accaatctat ttagttgtta taaaccttct tgaatgcttg ttcaaagtcg ttaataatat
     5221 catcaatata ttcagtacca gtagaaactc tgattaagcc cttggtaaca ccagaagcca
     5281 acttttcttc atcagacaat tgttgatgtg tagtaaacca tggagcaata accaaagtct
     5341 tagagtcacc cacattagcc aagtttgaag caatctccaa attgtcaacc aactttggtg
     5401 aggcttcatt gaatgggtcg ctggaggtgt tggtgatgtc cttgacagta aatgctaaag
     5461 caccaccaaa gtacttagcg tcattgttca agtatttttt actcaattcg tgggattcgt
     5521 gagatggcaa tcccaaatag gacacagact caacatttgg gttcttttct aaccattggg
     5581 ccaatttcaa agcattttca gattgtcttt caactctcaa agacaaagtt tccaaacctt
     5641 gcaacaacaa aaaacttcca aatggattca aagctggacc caagtctctc aacaattcaa
     5701 ttctcaagtg accaatgtat gcggccttac ctaaagcatc attcaagacc aacccgtggt
     5761 aaccttctga tggtttagag aattgtgggt atttttctgg gtactcggtc catgggaagt
     5821 taccggaatc aacaataaca ccagcaattg tagtaccgtg accaccaatc catttagtag
     5881 cagaatgaac aacaatatca gcaccgtggg caattgggtt aactaaaaat ccaccagcac
     5941 caaaagtgtt gtcgacaaca acaggaatac cgtgttcatg ggccaatttg gtgatttttt
     6001 caaagtctgg aacattatat ttagggtttc caatggtttc aatataaata gcttttgtct
     6061 tgtcgtcaat caatttagca aaatcttcag cggcgtcacc attaacgaat ttggtttcaa
     6121 tgcccaaacg tttgaaagca actttgaatt gattataagt accaccatac aagtaggatg
     6181 tactgataat gttatcacca gcatgagcca acccagcaat agccaagaat tgagctgatt
     6241 gaccagaaga agtggccaat gcaccaatac caccttccaa ggcagcaatt ctttgttcaa
     6301 acacatcgtt tgttggattc ataattctgg agtaaatgta tcctggggtt tctaaaccaa
     6361 ataattgagc accgtgttta gagtcattga aaacatagga ggtggttgca taaattggtg
     6421 gggctcttgg ttggtgtggt ttttcaactg gttgcccagc atgtaattga agtgtatcaa
     6481 agtgagaagg cattgtagtt tagtaatggg gaaaacaaaa aaggaagaat tgttggcctt
     6541 tttggccaag ctgactctag aggatccccg ggtaccgagc tcgaattgat ccggtaattt
     6601 agtgtgtgta tttgtgtttg cgtgtctata gaagtatagt aatttatgct acaaaggacc
     6661 taatgtataa ggaaagaata tttagagaaa agaagaaaac aagagtttta tatacataca
     6721 gagcacatgc atgccatatg atcatgtgtc gtcgcacaca tatatatatg cctgtatgtg
     6781 tcagcactaa agttgcctgg ccatccacgc tatatacacg cctggcggat ctgctcgagg
     6841 tcgattagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt
     6901 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
     6961 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
     7021 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
     7081 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
     7141 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
     7201 atttttgatc gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta
     7261 tacatgcatt tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg
     7321 cttcccagcc tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa
     7381 atagtcctct tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc
     7441 tatactgttg acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca
     7501 accaatcgta accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa
     7561 aatctttgtc gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg
     7621 agcccttgca tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt
     7681 ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa
     7741 tgtctgccca ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac
     7801 caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct
     7861 ttagcggctt aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta
     7921 gtaaacaaat tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa
     7981 catccaatga agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag
     8041 caacaggact aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc
     8101 ttcgtttccg gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc
     8161 ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc
     8221 cttctgttcg gagattaccg aatcaaaaaa atttcaaaga aaccgaaatc aaaaaaaaga
     8281 ataaaaaaaa aatgatgaat tgaattgaaa agctaattcg taatcatggt catagctgtt
     8341 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8401 gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt tgcgctcact
     8461 gcccgctttc cagtcgggaa acctgtcgtg ccanctggat taatgaatcg gccaacgcgc
     8521 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8581 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8641 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8701 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8761 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8821 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8881 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8941 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9001 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9061 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9361 gaacgaaaac tcacgttagg gattttggtc atgagattat caaaaaggat cttcacctag
     9421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9541 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9601 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9661 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9721 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9781 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9841 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9901 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9961 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10021 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10081 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10141 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10201 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10261 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10321 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10381 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10441 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    10501 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa
//
