LOCUS       V003413                 9906 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003413
VERSION     V003413
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9906)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9906)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    142..282
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(696..1209)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1697..1713
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1980..3071)
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     rep_origin      complement(3429..4775)
                     /direction=LEFT
                     /label="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     terminator      complement(4784..5031)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     misc_feature    complement(5114..5313)
                     /label="3' UTR of hIFNbeta"
                     /note="3' UTR of hIFNbeta"
     CDS             complement(5317..5877)
                     /gene="IFNB1"
                     /label="Interferon beta"
                     /note="Interferon beta from Homo sapiens. Accession#:
                     P01574"
     protein_bind    6267..6384
                     /label="UAS"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     CDS             complement(6644..7444)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(7445..7661)
                     /label="URA3 promoter"
     primer_bind     complement(7681..7697)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7705..7721)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7729..7759)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7774..7795)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8083..8671)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8844..9701)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9702..9806)
                     /label="AmpR promoter"
ORIGIN
        1 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta
      181 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      241 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata
      301 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag
      361 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta
      421 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt
      481 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa
      541 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga
      601 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga
      661 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc
      721 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa
      781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
      841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
      901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1201 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca
     1261 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa
     1321 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc
     1381 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa
     1441 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca
     1501 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat
     1561 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1621 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1681 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta
     1741 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt
     1801 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc
     1861 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta
     1921 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa
     1981 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa
     2041 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg
     2101 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata
     2161 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg
     2221 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa
     2281 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg
     2341 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca
     2401 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt
     2461 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat
     2521 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga
     2581 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca
     2641 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt
     2701 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg
     2761 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg
     2821 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct
     2881 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa
     2941 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta
     3001 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta
     3061 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc
     3121 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt
     3181 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag
     3241 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta
     3301 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa
     3361 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata
     3421 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     3481 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     3541 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     3601 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3661 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     3721 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     3781 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     3841 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     3901 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3961 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     4021 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     4081 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     4141 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     4201 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     4261 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     4321 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     4381 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac
     4441 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
     4501 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
     4561 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     4621 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     4681 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     4741 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg
     4801 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
     4861 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
     4921 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
     4981 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa
     5041 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg
     5101 ctaggcctcg agggactttt gcaccaaaaa taatttattt tccaaaataa aatttaaata
     5161 aataaaaata actcataatt taataaaaat ttcaaaatct tctagtgtcc tttcatatgc
     5221 agtacattag ccatcagtca cttaaacagc atctgctggt tgaagaatgc ttgaagcaat
     5281 tgtccagtcc cagaggcaca ggctaggaga tcttcagttt cggaggtaac ctgtaagtct
     5341 gttaatgaag taaaagttcc ttaggatttc cactctgact atggtccagg cacagtgact
     5401 gtactccttg gccttcaggt aatgcagaat cctcccataa tatcttttca ggtgcagact
     5461 gctcatgagt tttcccctgg tgaaatcttc tttctccagt ttttcttcca ggactgtctt
     5521 cagatggttt atctgatgat agacattagc caggaggttc tcaacaatag tctcattcca
     5581 gccagtgcta gatgaatctt gtctgaaaat agcaaagatg ttctggagca tctcatagat
     5641 ggtcaatgcg gcgtcctcct tctggaactg ctgcagctgc ttaatctcct cagggatgtc
     5701 aaagttcatc ctgtccttga ggcagtattc aagcctccca ttcaattgcc acaggagctt
     5761 ctgacactga aaattgctgc ttctttgtag gaatccaagc aagttgtagc tcatggaaag
     5821 agctgtagtg gagaagcaca acaggagagc aatttggagg agacacttgt tggtcatgtt
     5881 cctcgaggcc tttttggcca agctgactct agaggatccc cgggtaccga gctcgaattg
     5941 atccggtaat ttagtgtgtg tatttgtgtt tgcgtgtcta tagaagtata gtaatttatg
     6001 ctacaaagga cctaatgtat aaggaaagaa tatttagaga aaagaagaaa acaagagttt
     6061 tatatacata cagagcacat gcatgccata tgatcatgtg tcgtcgcaca catatatata
     6121 tgcctgtatg tgtcagcact aaagttgcct ggccatccac gctatataca cgcctggcgg
     6181 atctgctcga ggtcgattag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct
     6241 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc
     6301 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
     6361 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
     6421 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
     6481 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc
     6541 agcgaagcga tgatttttga tcgggtaata actgatataa ttaaattgaa gctctaattt
     6601 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
     6661 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
     6721 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
     6781 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
     6841 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
     6901 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
     6961 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
     7021 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
     7081 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
     7141 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
     7201 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
     7261 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
     7321 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
     7381 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
     7441 acatgattta tcttcgtttc cggtttttgt tctgtgcagt tgggttaaga atactgggca
     7501 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
     7561 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa
     7621 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctaatt cgtaatcatg
     7681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
     7801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccanctgg attaatgaat
     7861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8701 tgacgctcag tggaacgaaa actcacgtta gggattttgg tcatgagatt atcaaaaagg
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9841 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9901 cttcaa
//
