LOCUS       40924_38948        4464 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCANS-3-BNL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Dunn JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2000) Biology Department, Brookhaven National 
            Laboratory, 463 Bell Ave., Upton, NY 11973, USA
REFERENCE   2  (bases 1 to 4464)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-AUG-2000) Biology Department, Brookhaven National Laboratory, 
            463 Bell Ave., Upton, NY 11973, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..813
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        961..979
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1133..1151)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1162..1178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1274..1322)
                     /direction=LEFT
                     /label=f1
                     /note="f1"
     misc_feature    complement(1396..1646)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(1652..2404)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(2495..2714)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    3134..3155
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3170..3200
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3208..3224
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3232..3248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3321..4166
                     /codon_start=1
                     /gene="repL"
                     /product="RepL"
                     /label=repL
                     /protein_id="AAG12977.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     gene            3321..4166
                     /gene="repL"
                     /label=repL
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata aactgttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      841 cactggcgag ctcggatcgc ggccgtcgct accttaggac cgttatagta gggataacag
      901 ggtaatggcg ccgacgtcgg ccgaggcccg ggcgtttaaa catttaaatg tcgacatacg
      961 atttaggtga cactatagaa ctctaatacg actcactata gggaatttgg ccctcgaggc
     1021 caagaattcc cgactacgta gtcggggatc catacataag ttggttaacc attctaaacg
     1081 tgggatccat gatcatggcg gccgcaagct taattaacct ggtagcttat tccctttagt
     1141 gagggttaat tttagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1201 tgttaaccgg cgcgccacgc gtacccataa tacccataat agctgtttgc catcgcgtat
     1261 gcatcgatca cgtgtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1321 tcggatcgat ccggcgcgca ccgtgggaaa aactccaggt agaggtacac acgcggatag
     1381 ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga actaaccccc
     1441 gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa actgccctca
     1501 aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg caggctgaag
     1561 gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg acgaaccacc
     1621 ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg atatcgcgga
     1681 aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat gagaggcgca
     1741 ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg cggaagtcag
     1801 gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg attttcagag
     1861 agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat aaacgcatgg
     1921 catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac cggttctgta
     1981 acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc cctctggatg
     2041 gactgtgcgc acgtttgata aaccaaggaa aagattcata gcctttttca tcgccggcat
     2101 cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat gcctgccgta
     2161 tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta gcaacatgga
     2221 tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc tggtcaacga
     2281 acagatacag catacgtttt tgatcccggg agagactata tgccgcctca gtgaggtcgt
     2341 ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc gctgtttccg
     2401 ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa ataaaaaaga
     2461 gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag
     2521 ggttaagggc aatttgtcac agacaggact gtcatttgag ggtgatttgt cacactgaaa
     2581 gggcaatttg tcacaacacc ttctctagaa ccagcatgga taaaggccta caaggcgctc
     2641 taaaaaagaa gatctaaaaa ctataaaaaa aataattata aaaatatccc cgtggataag
     2701 tggataaccc caagggaagt tttttcaggc atcgtgtgta agcagaatat ataagtgctg
     2761 ttccctggtg cttcctcgct cactcgaaat tcccggggat agctttatgc ttgtaaaccg
     2821 ttttgtgaaa aaatttttaa aataaaaaag gggacctcta gggtccccaa ttaattagta
     2881 atataatcta ttaaaggtca ttcaaaaggt catccaccgg atcaattccc ctgctcgcgc
     2941 aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc
     3001 cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca
     3061 ctgatccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3121 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3181 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3241 gctatgacca tgattacgcc aagcgcggta cctgtcgcgg caacgcgcta acagacgtag
     3301 taagaaccac cagcattgta atgctggcta aagtcacttt cctgagctgt ataacgatga
     3361 gcgattttac tttttctggc tatgaattgg cctgctttgt aacacactcc ggtctatccc
     3421 gtagcgccgg gcatatcctg tcgcaatgtg caaatctcgc ggcaacaacc agtgaatact
     3481 tcattcacaa gcctcaccgc ctgatcgcgg cagaaactgg ttatagccaa tcaaccgtcg
     3541 ttcgtgcatt ccgtgaagct gtaaacaaag gtatcctgtc tgtagagatt gttatcggcg
     3601 atcaccgtga acgtcgcgct aacctgtacc ggtttacacc atcctttttg gccttcgcac
     3661 aacaagccaa aaatgcgctg atagaaagca aattaaagat ctcttcagcg gcaaccaagg
     3721 ttaaagctgt tctcgctaag acattggctt tatttaattt tttatccaca cccccatgtc
     3781 aaaatgatac cccctccccc tgtcaggatg acgtggcaat aaagaataag aagtcacaag
     3841 ttaaaaaaac aaaaagatca gtttccggcg gtgccggaac aaccagcctc aaaaaattga
     3901 cttcatggat cgctaaggca aaagcaaagg ctgacaatct gcggttatcc aaaaaacgca
     3961 ctcaaaaaca tgagttcaag cagaaagtag aggcggctgc gcggaaatat gcttacctga
     4021 agaacaagcg ttcgcctgat attggcggga tatcaaactt cgataaccta ccgcattgca
     4081 tgacggtaaa cgaagctctt aatgcggttt tagccaaaaa taaagataac gaacaatggg
     4141 gtataccggc aggattcaga gggtaatgaa ttgctctaat tataaccatg catactttca
     4201 acacctctag tttgccatga ggcaaactca taggtgtcct ggtaagagga cactgttgcc
     4261 aaaactggac gccccattat tgcaattaat aaacaactaa cggacaattc tacctaacaa
     4321 taagtggagt tgcggccggc cgcgatccgc tagcaaagcc acgttgtgtc tcaaaatctc
     4381 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4441 ataaacagta atacaagggg tgtt
//
