LOCUS       40924_38943        4428 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCANS-2-BNL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Dunn JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2000) Biology Department, Brookhaven National 
            Laboratory, 463 Bell Ave., Upton, NY 11973-5000, USA
REFERENCE   2  (bases 1 to 4428)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-AUG-2000) Biology Department, Brookhaven National Laboratory, 
            463 Bell Ave., Upton, NY 11973-5000, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..813
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        961..979
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1097..1115)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1126..1142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1238..1286)
                     /direction=LEFT
                     /label=f1
                     /note="f1"
     misc_feature    complement(1360..1610)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(1616..2368)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(2459..2678)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    3098..3119
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3134..3164
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3172..3188
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3196..3212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3285..4130
                     /codon_start=1
                     /gene="repL"
                     /product="RepL"
                     /label=repL
                     /protein_id="AAG27439.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     gene            3285..4130
                     /gene="repL"
                     /label=repL
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata aactgttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      841 cactggcgag ctcggatcgc ggccgtcgct accttaggac cgttatagta gggataacag
      901 ggtaatggcg ccgacgtcgg ccgaggcccg ggcgtttaaa catttaaatg tcgacatacg
      961 atttaggtga cactatagaa ctctaatacg actcactata gggaatttgg ccctcgaggc
     1021 caagaattcc cgactacgta gtcggggatc cgcatgcata tgcggccgca agcttaatta
     1081 acctggtagc ttattccctt tagtgagggt taattttagc ttggcactgg ccgtcgtttt
     1141 acaacgtcgt gactgggaaa accctgttaa ccggcgcgcc acgcgtaccc ataataccca
     1201 taatagctgt ttgccatcgc gtatgcatcg atcacgtgtc cacgttcttt aatagtggac
     1261 tcttgttcca aactggaaca acactcggat cgatccggcg cgcaccgtgg gaaaaactcc
     1321 aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata atcaaccctc
     1381 atcaatgatg acgaactaac ccccgatatc aggtcacatg acgaagggaa agagaaggaa
     1441 atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt tcaaaaagta
     1501 tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac cctcagtagg
     1561 tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca gactatcctg
     1621 tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg cggcctttct
     1681 ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta acacagacct
     1741 gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc tggtaacgct
     1801 ctatgatcca gtcgattttc agagagacga tgcctgagcc atccggctta cgatactgac
     1861 acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt tcactaagcc
     1921 gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat gggttgatat
     1981 gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccaa ggaaaagatt
     2041 catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact tccttccccg
     2101 cgaaactctt caatgcctgc cgtatatcct tactggcttc cgcagaggtc aatccgaata
     2161 tttcagcata tttagcaaca tggatctcgc agataccgtc atgttcctgt agggtgccat
     2221 cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc cgggagagac
     2281 tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt ttacgtttct
     2341 tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtgtga caagttttta
     2401 gattgtcaca ctaaataaaa aagagtcaat aagcagggat aactttgtga aaaaacagct
     2461 tcttctgagg gcaatttgtc acagggttaa gggcaatttg tcacagacag gactgtcatt
     2521 tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa caccttctct agaaccagca
     2581 tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa aaaaaataat
     2641 tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc aggcatcgtg
     2701 tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg aaattcccgg
     2761 ggatagcttt atgcttgtaa accgttttgt gaaaaaattt ttaaaataaa aaaggggacc
     2821 tctagggtcc ccaattaatt agtaatataa tctattaaag gtcattcaaa aggtcatcca
     2881 ccggatcaat tcccctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc
     2941 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc
     3001 ttgacccgca gttgcaaacc ctcactgatc cgattcatta atgcagctgg cacgacaggt
     3061 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     3181 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc ggtacctgtc
     3241 gcggcaacgc gctaacagac gtagtaagaa ccaccagcat tgtaatgctg gctaaagtca
     3301 ctttcctgag ctgtataacg atgagcgatt ttactttttc tggctatgaa ttggcctgct
     3361 ttgtaacaca ctccggtcta tcccgtagcg ccgggcatat cctgtcgcaa tgtgcaaatc
     3421 tcgcggcaac aaccagtgaa tacttcattc acaagcctca ccgcctgatc gcggcagaaa
     3481 ctggttatag ccaatcaacc gtcgttcgtg cattccgtga agctgtaaac aaaggtatcc
     3541 tgtctgtaga gattgttatc ggcgatcacc gtgaacgtcg cgctaacctg taccggttta
     3601 caccatcctt tttggccttc gcacaacaag ccaaaaatgc gctgatagaa agcaaattaa
     3661 agatctcttc agcggcaacc aaggttaaag ctgttctcgc taagacattg gctttattta
     3721 attttttatc cacaccccca tgtcaaaatg ataccccctc cccctgtcag gatgacgtgg
     3781 caataaagaa taagaagtca caagttaaaa aaacaaaaag atcagtttcc ggcggtgccg
     3841 gaacaaccag cctcaaaaaa ttgacttcat ggatcgctaa ggcaaaagca aaggctgaca
     3901 atctgcggtt atccaaaaaa cgcactcaaa aacatgagtt caagcagaaa gtagaggcgg
     3961 ctgcgcggaa atatgcttac ctgaagaaca agcgttcgcc tgatattggc gggatatcaa
     4021 acttcgataa cctaccgcat tgcatgacgg taaacgaagc tcttaatgcg gttttagcca
     4081 aaaataaaga taacgaacaa tggggtatac cggcaggatt cagagggtaa tgaattgctc
     4141 taattataac catgcatact ttcaacacct ctagtttgcc atgaggcaaa ctcataggtg
     4201 tcctggtaag aggacactgt tgccaaaact ggacgcccca ttattgcaat taataaacaa
     4261 ctaacggaca attctaccta acaataagtg gagttgcggc cggccgcgat ccgctagcaa
     4321 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     4381 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt
//
