LOCUS       40924_38938        4427 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCANS-1-BNL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Dunn JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-1999) Biology Dept., Brookhaven National 
            Laboratory, 463 Bell Ave., Upton, NY 11973-5000, USA
REFERENCE   2  (bases 1 to 4427)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4427)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-1999) Biology Dept., Brookhaven National Laboratory, 463 
            Bell Ave., Upton, NY 11973-5000, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..813
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        961..979
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1096..1114)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1125..1141)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1237..1285)
                     /direction=LEFT
                     /label=f1
                     /note="f1"
     misc_feature    complement(1359..1609)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(1615..2367)
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      complement(2458..2677)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    3097..3118
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3133..3163
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3171..3187
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3195..3211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3284..4129
                     /codon_start=1
                     /gene="repL"
                     /product="RepL"
                     /label=repL
                     /protein_id="AAF23897.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     gene            3284..4129
                     /gene="repL"
                     /label=repL
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata aactgttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      841 cactggcgag ctcggatcgc ggccgtcgct accttaggac cgttatagta gggataacag
      901 ggtaatggcg ccgacgtcgg ccgaggcccg ggcgtttaaa catttaaatg tcgacatacg
      961 atttaggtga cactatagaa ctctaatacg actcactata gggaatttgg ccctcgaggc
     1021 caagaattcc cgactacgta gtcggggatc catgatcatg gcggccgcaa gcttaattaa
     1081 cctggtagct tattcccttt agtgagggtt aattttagct tggcactggc cgtcgtttta
     1141 caacgtcgtg actgggaaaa ccctgttaac cggcgcgcca cgcgtaccca taatacccat
     1201 aatagctgtt tgccatcgcg tatgcatcga tcacgtgtcc acgttcttta atagtggact
     1261 cttgttccaa actggaacaa cactcggatc gatccggcgc gcaccgtggg aaaaactcca
     1321 ggtagaggta cacacgcgga tagccaattc agagtaataa actgtgataa tcaaccctca
     1381 tcaatgatga cgaactaacc cccgatatca ggtcacatga cgaagggaaa gagaaggaaa
     1441 tcaactgtga caaactgccc tcaaatttgg cttccttaaa aattacagtt caaaaagtat
     1501 gagaaaatcc atgcaggctg aaggaaacag caaaactgtg acaaattacc ctcagtaggt
     1561 cagaacaaat gtgacgaacc accctcaaat ctgtgacaga taaccctcag actatcctgt
     1621 cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt
     1681 tttctcaatg tatgagaggc gcattggagt tctgctgttg atctcattaa cacagacctg
     1741 caggaagcgg cggcggaagt caggcatacg ctggtaactt tgaggcagct ggtaacgctc
     1801 tatgatccag tcgattttca gagagacgat gcctgagcca tccggcttac gatactgaca
     1861 cagggattcg tataaacgca tggcatacgg attggtgatt tcttttgttt cactaagccg
     1921 aaactgcgta aaccggttct gtaacccgat aaagaaggga atgagatatg ggttgatatg
     1981 tacactgtaa agccctctgg atggactgtg cgcacgtttg ataaaccaag gaaaagattc
     2041 atagcctttt tcatcgccgg catcctcttc agggcgataa aaaaccactt ccttccccgc
     2101 gaaactcttc aatgcctgcc gtatatcctt actggcttcc gcagaggtca atccgaatat
     2161 ttcagcatat ttagcaacat ggatctcgca gataccgtca tgttcctgta gggtgccatc
     2221 agattttctg atctggtcaa cgaacagata cagcatacgt ttttgatccc gggagagact
     2281 atatgccgcc tcagtgaggt cgtttgactg gacgattcgc gggctatttt tacgtttctt
     2341 gtgattgata accgctgttt ccgccatgac agatccatgt gaagtgtgac aagtttttag
     2401 attgtcacac taaataaaaa agagtcaata agcagggata actttgtgaa aaaacagctt
     2461 cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg actgtcattt
     2521 gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta gaaccagcat
     2581 ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa aaaaataatt
     2641 ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca ggcatcgtgt
     2701 gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga aattcccggg
     2761 gatagcttta tgcttgtaaa ccgttttgtg aaaaaatttt taaaataaaa aaggggacct
     2821 ctagggtccc caattaatta gtaatataat ctattaaagg tcattcaaaa ggtcatccac
     2881 cggatcaatt cccctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc
     2941 gatccttgga gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct
     3001 tgacccgcag ttgcaaaccc tcactgatcc gattcattaa tgcagctggc acgacaggtt
     3061 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg gtacctgtcg
     3241 cggcaacgcg ctaacagacg tagtaagaac caccagcatt gtaatgctgg ctaaagtcac
     3301 tttcctgagc tgtataacga tgagcgattt tactttttct ggctatgaat tggcctgctt
     3361 tgtaacacac tccggtctat cccgtagcgc cgggcatatc ctgtcgcaat gtgcaaatct
     3421 cgcggcaaca accagtgaat acttcattca caagcctcac cgcctgatcg cggcagaaac
     3481 tggttatagc caatcaaccg tcgttcgtgc attccgtgaa gctgtaaaca aaggtatcct
     3541 gtctgtagag attgttatcg gcgatcaccg tgaacgtcgc gctaacctgt accggtttac
     3601 accatccttt ttggccttcg cacaacaagc caaaaatgcg ctgatagaaa gcaaattaaa
     3661 gatctcttca gcggcaacca aggttaaagc tgttctcgct aagacattgg ctttatttaa
     3721 ttttttatcc acacccccat gtcaaaatga taccccctcc ccctgtcagg atgacgtggc
     3781 aataaagaat aagaagtcac aagttaaaaa aacaaaaaga tcagtttccg gcggtgccgg
     3841 aacaaccagc ctcaaaaaat tgacttcatg gatcgctaag gcaaaagcaa aggctgacaa
     3901 tctgcggtta tccaaaaaac gcactcaaaa acatgagttc aagcagaaag tagaggcggc
     3961 tgcgcggaaa tatgcttacc tgaagaacaa gcgttcgcct gatattggcg ggatatcaaa
     4021 cttcgataac ctaccgcatt gcatgacggt aaacgaagct cttaatgcgg ttttagccaa
     4081 aaataaagat aacgaacaat ggggtatacc ggcaggattc agagggtaat gaattgctct
     4141 aattataacc atgcatactt tcaacacctc tagtttgcca tgaggcaaac tcataggtgt
     4201 cctggtaaga ggacactgtt gccaaaactg gacgccccat tattgcaatt aataaacaac
     4261 taacggacaa ttctacctaa caataagtgg agttgcggcc ggccgcgatc cgctagcaaa
     4321 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     4381 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtt
//
