LOCUS       40924_38843        6982 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon delivery vector pSC189, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Chiang SL, Rubin EJ.
  TITLE     Construction of a mariner-based transposon for epitope-tagging and 
            genomic targeting
  JOURNAL   Gene 296 (1-2), 179-185 (2002)
  PUBMED    12383515
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Chiang SL, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2002) Microbiology, Harvard Medical School, 200 
            Longwood Avenue, Armenise I-427, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 6982)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6982)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 296 
            (1-2), 179-185 (2002)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2002) Microbiology, Harvard Medical School, 200 Longwood 
            Avenue, Armenise I-427, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             171..1217
                     /codon_start=1
                     /product="mariner transposase C9 mutant"
                     /label=mariner transposase C9 mutant
                     /protein_id="AAM82289.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     promoter        complement(1280..1298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1635..1656
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1671..1701
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1709..1725
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1733..1749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1770..1788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1801..1827
     CDS             1843..1908
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     protein_bind    1912..1959
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(1978..1996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2204..2592
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(2729..2745)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3143..3934
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    3971..4018
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     repeat_region   4032..4058
     promoter        complement(4071..4089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4096..4112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4681..5049)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5082..5191)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        5861..5965
                     /label=AmpR promoter
     CDS             5966..6823
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ccgccagtgt gatggattga cacatagatg gcgtcgctag tattaaatgc atattatttt
       61 tatatagtac caaccttcaa atgattcgtg tcaaaatttg acgtctgtaa gtcaattagt
      121 ttgtgagata gagcgtcttt tgtgaagcaa cttttgttat tgtgaaacat atggaaaaaa
      181 aggaatttcg tgttttgata aaatactgtt ttctgaaggg aaaaaataca gtggaagcaa
      241 aaacttggct tgataatgag tttccggact ctgccccagg gaaatcaaca ataattgatt
      301 ggtatgcaaa attcaagcgt ggtgaaatga gcacggagga cggtgaacgc agtggacgcc
      361 cgaaagaggt ggttaccgac gaaaacatca aaaaaatcca caaaatgatt ttgaatgacc
      421 gtaaaatgaa gttgatcgag atagcagagg ccttaaagat atcaaaggaa cgtgttggtc
      481 atatcattca tcaatatttg gatatgcgga agctctgtgc gaaatgggtg ccgcgcgagc
      541 tcacatttga ccaaaaacaa cgacgtgttg atgattctaa gcggtgtttg cagctgttaa
      601 ctcgtaatac acccgagttt ttccgtcgat atgtgacaat ggatgaaaca tggctccatc
      661 actacactcc tgagtccaat cgacagtcgg ctgagtggac agcgaccggt gaaccgtctc
      721 cgaagcgtgg aaagactcaa aagtccgctg gcaaagtaat ggcctctgtt ttttgggatg
      781 cgcatggaat aatttttatc gattatcttg agaagggaaa aaccatcaac agtgactatt
      841 atatggcgtt attggagcgt ttgaaggtcg aaatcgcggc aaaacggccc cacatgaaga
      901 agaaaaaagt gttgttccac caagacaacg caccgtgcca caagtcattg agaacgatgg
      961 caaaaattca tgaattgggc ttcgaattgc ttccccaccc accgtattct ccagatctgg
     1021 cccccagcga ctttttcttg ttctcagacc tcaaaaggat gctcgcaggg aaaaaatttg
     1081 gctgcaatga agaggtgatc gccgaaactg aggcctattt tgaggcaaaa ccgaaggagt
     1141 actaccaaaa tggtatcaaa aaattggaag gtcgttataa tcgttgtatc gctcttgaag
     1201 ggaactatgt tgaataataa aaacgaattt tgacaaaaaa atgtgttttt ctttgttaga
     1261 ccggaatcct ccaattcgcc ctatagtgag tcgtattacg cgcgggcttt ccccgtcaag
     1321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     1381 aaaaacttga ttagggtgat ggttcacttc tttcctgcgt tatcccctga ttctgtggat
     1441 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1501 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1561 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1621 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     1681 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     1741 agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg
     1801 acaggttggc tgataagtcc ccggtctcta gaattcacca tagactacaa agaccatgac
     1861 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagct tgaagttcct
     1921 attccgaagt tcctattctc tagaaagtat aggaacttcg gcgcgccgct ctagacccct
     1981 atagtgagtc gtattacaaa ctggaacaac actcaaccct atctcggtct attcttttga
     2041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2101 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     2161 aatgtgcgcg gaaccagatc cccacgatgc gtccggcgta gaggatctga agatcagcag
     2221 ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     2281 aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc
     2341 atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa
     2401 attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa
     2461 gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat
     2521 gtaacgcact gagaagccct tagagcctct caaagcaatt ttcagtgaca caggaacact
     2581 taacggctga cagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     2641 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gttaattaag
     2701 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     2761 ccgaggacgc gtcgaattaa ttccgctagc ttcacgctgc cgcaagcact cagggcgcaa
     2821 gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg
     2881 gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca
     2941 ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag
     3001 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa
     3061 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga
     3121 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3181 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3241 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3301 gtccggtgcc ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac
     3361 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3421 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3481 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3541 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3601 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     3661 gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     3721 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     3781 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     3841 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     3901 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gtacgcgtct
     3961 aggcggccgc gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcga
     4021 accggttcta gagaccgggg acttatcagc caacctgtta gccaattcgc cctatagtga
     4081 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4141 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4201 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4261 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4321 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4381 ccgcgcaacg tctccgcgtc gggcttctct cccgcgcgga agctaaccag caggtgatag
     4441 gtcttgtcgg cctcggaacg ggtgttgccg tgctgggtcg ccatcacctc ggccatgaca
     4501 gcgggcaggg tgtttgcctc gcagttcgtg acgcgcacgt gacccaggcg ctcggtcttg
     4561 ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc
     4621 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     4681 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     4741 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     4801 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     4861 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     4921 cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     4981 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     5041 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     5101 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     5161 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     5221 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     5281 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct gcttccctgc
     5341 tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaattac tgaactgagg
     5401 ggacaggcga gagacgatgc caaagagcta caccgacgag ctggccgagt gggttgaatc
     5461 ccgcgcggcc aagaagcgcc ggcgtgatga ggctgcggtt gcgttcctgg cggtgagggc
     5521 ggatgtcgag gcggcgttag cgtccggcta tgcgctcgtc accatttggg agcacatgcg
     5581 ggaaacgggg aaggtcaagt tctcctacga gacgttccgc tcgcacgcca ggcggcacat
     5641 caaggccaag cccgccgatg tgcccgcacc gcaggccaag gctgcggaac ccgcgccggc
     5701 acccaagacg ccggagccac ggcggccgaa gcaggggggc aaggctgaaa agccggcccc
     5761 cgctgcggcc ccgaccggct tcaccttcaa cccaacaccg gacaaaaagg atccactagt
     5821 tctagctaga gcggccgctg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5881 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5941 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6001 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6061 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6121 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6181 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6241 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6301 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6361 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6421 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6481 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6541 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6601 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6661 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6721 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6781 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6841 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6901 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6961 agcgtcagac cccgtagagc gg
//
