LOCUS       62056_19375        5100 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pSC101-mKate2-AattB-AattP-BFP, complete sequence.
ACCESSION   MN623112
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..5100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(185..880)
                     /codon_start=1
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
                     /translation="MLSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     promoter        complement(967..1001)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      1018..1089
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             1183..1881
                     /codon_start=1
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     terminator      1890..1917
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1959..1990
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      2115..2209
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2701..2923
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2971..3918
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNCAKFS
                     SEKLELVFSEGILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQFTSFEAKF
                     LGDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             4210..5067
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITTSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5081..5100
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 ctcaacgatg tcctaatttc gacggaaggt cacaataacg ggctacggct acttcatgtt
      241 gttcaacgta ggtttcttta tcagcttctt taatacgttc taagcgacga tcaacatagt
      301 atactccagg cattttaagg tttttagcgg gttttttgga acgataggtt gttttcaggt
      361 tgcagattaa atgaccgcct ccaactaatt tcagtgccat gtctgcgcgt ccttctaagc
      421 caccgtccgc aggatataag gtttctgttg atgcttccca accaagagtt tttttttgca
      481 ttacgggtcc gttactcggg aaatttacgc cacggatttt aacattgtaa atcagacagc
      541 catcttgtaa actcgtatct tgagttgctg taagtacacc gccatcttca taagttgtta
      601 cacgttccca ggtaaagcct tcaggaaagg actgtttaaa aaagtcagga atgccttggg
      661 tgtgattgat aaaagtttta gacccgtaca taaagctcgt tgcgagaatg tcaaacgcaa
      721 agggcagggg tccaccttca actgctttga tgcgcattgt ttgtgtgcct tcatatggtt
      781 taccttcacc ttcggatgtg catttgaaat gatgattgtt gacagtacct tccatatata
      841 atttcatgtg catattttct ttaattaatt ctgagagcat gatatctttc tcctctttaa
      901 tgcttaactt ttcggatcaa gctatgaagg acgcaaagag ggaactaaac acttggatcc
      961 cccggggcta gcattatacc taggactgag ctagctgtca atactagagc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagagg tcatatgttt agtttctcgt ttcttcttct tccaacgaga
     1141 gaaaacgagg aactaaggta ccttaaagag gagaaagtcg acatgagcga gctgattaag
     1201 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     1261 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     1321 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     1381 aagaccttca tcgaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     1441 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1501 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1561 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     1621 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     1681 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     1741 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     1801 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     1861 aaactggggc acaagctcaa ttaagatctg gctcaccttc gggtgggcct ttctgcgctg
     1921 caggagtcac taagggttag ttagttagat tagcagaaag tcaaaagcct ccgaccggag
     1981 gcttttgact aaaacttccc ttggggttat cattggggct cactcaaagg cggtaatcag
     2041 ataaaaaaaa tccttagctt tcgctgagga tgatttctgc tagagctgtc agaccaagtt
     2101 tacgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga
     2161 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcactag
     2221 ggacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg cattagccag
     2281 tctgaatgac ctgtcacggg gtaatccgaa gtggccagac tggaaaatca gagggcagga
     2341 actgctgaac agcaaaaagt cagatagcac cgcatagcag acccgccata aaacgccctg
     2401 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
     2461 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagtgca gcgaactgaa
     2521 tgtcacgaaa aagacggcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
     2581 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     2641 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     2701 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     2761 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     2821 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     2881 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
     2941 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     3001 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     3061 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     3121 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     3181 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     3241 ggctttgaga ttttccagtg gacaaactgt gccaagttct caagcgaaaa attagaatta
     3301 gtttttagtg aagggatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     3361 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     3421 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     3481 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     3541 ttgaaaccaa taagtaaaga tttgaacact tacagcaata tgaaattggt ggttgataag
     3601 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     3661 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     3721 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     3781 aaaattcagt tcaccagttt tgaggcaaaa tttttgggtg acatgcaaag taagtatgat
     3841 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     3901 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     3961 actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa aactgtccat
     4021 atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac gagtttttgg
     4081 tgcattcaaa gctgttcacc atgaacagat cgacaatgta actactagag ctaaatacat
     4141 tcaaatatct atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4201 aggaagaata tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4261 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc cgaagatcag
     4321 ttgggcgcac gtgtgggtta catcgaactg gacctcaaca gcggtaagat tcttgagagt
     4381 tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct ctgtggcgcg
     4441 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4501 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggacgg catgacagta
     4561 cgcgaattat gcagcgctgc cataaccacg agtgataaca cggcggccaa cttacttctg
     4621 acaacgatcg ggggaccgaa ggagcttacc gcttttttgc acaacatggg tgatcatgta
     4681 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4741 accacgatgc ctgtagctat ggcaacaacg ttgcgcaaac tcttaactgg cgaacttctt
     4801 actctcgctt cccggcaaca attaatagac tggatggagg cggataaagt tgcgggacca
     4861 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4921 cgtgggtccc gcggtattat tgcagccctg gggccagatg gtaagccctc ccgtatcgta
     4981 gttatctaca cgacggggag ccaggcaact atggacgaac gtaatcgcca gatcgctgag
     5041 ataggtgcct ccctgattaa gcattggtaa taatactagc tccggcaaaa aaacgggcaa
//
